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243901“…Samples were quantified by tissue culture infectious dose(50)(TCID(50))/mL or plaque assay (cell line dependent) and additionally analyzed on the Sartorius Virus Counter 3100 (VC), which uses flow virometry to count the total number of intact virus particles in a sample. …”
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243902“…We profiled the ncRNAs (non-coding RNA) present in the EVs from seven dairy products - raw whole milk, heat-treated skim milk, homogenized heat-treated skim milk, pasteurized homogenized skim milk, pasteurized heavy whipping cream, sweet cream buttermilk and cultured buttermilk with four replicates each, obtained at different processing steps from a commercial dairy plant. …”
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243903por Sánchez-López, Edwin F., Corigliano, Mariana G., Oliferuk, Sonia, Ramos-Duarte, Victor A., Rivera, Maximiliano, Mendoza-Morales, Luisa F., Angel, Sergio O., Sander, Valeria A., Clemente, Marina“…When comparing the transient expression in Nicotiana tabacum X-27-8 leaves, which overexpress the suppressor helper component protease HC-Pro-tobacco etch virus (TEV), to that in N. benthamiana leaves, co-agroinfiltrated with the suppressor p19, optimal conditions included 6-week-old N. benthamiana plants, 7-day time to harvest, Agrobacterium tumefaciens cultures with an OD(600nm) of 0.6 for binary vectors and LED lights. …”
Publicado 2021
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243904por Liu, Mengru, Yang, Peng, Fu, Dongliang, Gao, Tong, Deng, Xinyi, Shao, Mingjing, Liao, Jiangquan, Jiang, Hong, Li, Xianlun“…In vitro study, LPS stimulated Tie2 expressing macrophages were cultured in an ischemic buffer. We evaluated the accumulation of VEGF by fura-2/AM fluorescence. …”
Publicado 2021
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243905por Morgenstern, Jakob, Groener, Jan B., Jende, Johann M. E., Kurz, Felix T., Strom, Alexander, Göpfert, Jens, Kender, Zoltan, Le Marois, Maxime, Brune, Maik, Kuner, Rohini, Herzig, Stephan, Roden, Michael, Ziegler, Dan, Bendszus, Martin, Szendroedi, Julia, Nawroth, Peter, Kopf, Stefan, Fleming, Thomas“…METHODS: Schwann cells were cultured under high-glucose conditions and mRNAs of various myelin-specific genes were screened intra- and extracellularly. …”
Publicado 2021
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243906por Doukani, Asmae, Cerga Pashoja, Arlinda, Fanaj, Naim, Qirjako, Gentiana, Meksi, Andia, Mustafa, Sevim, Vis, Christiaan, Hug, Juliane“…However, a range of barriers was also highlighted in relation to accessing the target treatment population, a culture of mental health stigma, underdeveloped information and communications technology infrastructure, and limited appropriately trained health care workforce, which reduce organizational readiness for implementing iCBT for depression. …”
Publicado 2021
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243907por Hannachi, Sami, Werbrouck, Stefaan, Bahrini, Insaf, Abdelgadir, Abdelmuhsin, Affan Siddiqui, Hira“…After three years of in vivo culture, we evaluated the impact of NaCl stress on agronomic, physiological and biochemical parameters compared to the parental control (‘P’). …”
Publicado 2021
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243908por Xia, Hui, Zheng, Yang, Liu, Dongxin, Wang, Shengfen, He, WenCong, Zhao, Bing, Song, Yuanyuan, Ou, Xichao, Zhou, Yang, van den Hof, Susan, Cobelens, Frank, Zhao, YanLin“…We assessed moxifloxacin resistance among MDR-TB isolates collected in national drug resistance surveys in 2007 and 2013 that included 3,634 smear-positive and 7,206 culture-positive pulmonary tuberculosis patients, respectively. …”
Publicado 2021
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243909por Shafrin, Jason, Marijam, Alen, Joshi, Ashish V, Mitrani-Gold, Fanny S, Everson, Katie, Tuly, Rifat, Rosenquist, Peter, Gillam, Michael, Ruiz, Maria Elena“…Patients included were female, aged ≥ 12 years with incident uUTI (diagnosis code or urine culture), and given an oral AB ± 5 days of diagnosis and ≥ 1 antibiotic susceptibility test. …”
Publicado 2021
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243910“…Mice that died prior to day 15, were too sick to provide samples, or had positive stool culture were considered positive for day 15 spore shedding. …”
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243911por LeBlanc, Jason J, ElSherif, May, Ye, Lingyun, MacKinnon-Cameron, Donna, Ambrose, Ardith, Hatchette, Todd F, Lang, Amanda L S, Gillis, Hayley D, Martin, Irene, Demczuk, Walter, Andrew, Melissa K, Boivin, Guy, Bowie, William, Green, Karen, Johnstone, Jennie, Loeb, Mark, McCarthy, Anne, McGeer, Allison, Semret, Makeda, Trottier, Sylvie, Valiquette, Louis, Webster, Duncan, McNeil, Shelly“…Streptococcus pneumoniae was detected using blood and sputum culture, or urine antigen detection (UAD). Serotype was assigned using Quellung reaction, PCR, or serotype-specific UADs spanning the 24 serotypes in PCV13 and PPV23 vaccines. …”
Publicado 2021
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243912por Henry, Molly, Puzniak, Laura A, Harris, Kelly, Van Schooneveld, Trevor C, Van Schooneveld, Trevor C, Bergman, Scott J, Bergman, Scott J“…Median time to initiation for all courses was 2 days after obtaining cultures and median duration was 7 days. 12 patients were on CVVHD (median flow rate 2.5L/hr) and 7 were on iHD. 2 patients received iHD after CVVHD. …”
Publicado 2021
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243913por Arends, S J Ryan, Butler, Deborah, Scangarella-Oman, Nicole, Paustian, Lindsey, Streit, Jennifer M, Mendes, Rodrigo E“…Most isolates (68%) tested were cultured from urine specimens collected from patients seen in ambulatory, emergency, family practice, and outpatient medical services. …”
Publicado 2021
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243914por Ötleş, Erkin, Oh, Jeeheh, Patel, Alieysa, Keidan, Micah, Young, Vincent B, Rao, Krishna, Wiens, Jenna“…We performed anaerobic culture on the residual media followed by a custom, multiplex PCR on isolates to identify toxigenic C. difficile. …”
Publicado 2021
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243915por Huang, Wen-Chien, Yadav, Vijesh Kumar, Cheng, Wei-Hong, Wang, Chun-Hua, Hsieh, Ming-Shou, Huang, Ting-Yi, Lin, Shiou-Fu, Yeh, Chi-Tai, Kuo, Kuang-Tai“…Osimertinib-sensitive and resistant NSCLC cells obtained from patients were used to co-culture with human lung fibroblasts (HLFs) to generate CAF cells (termed CAF_R1 and CAF_S1), and the functional roles of these CAF cells plus the regulatory mechanisms were further explored. …”
Publicado 2021
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243916por Vattulainen, Meri, Ilmarinen, Tanja, Viheriälä, Taina, Jokinen, Vilma, Skottman, Heli“…Human and porcine tissue-derived cultured LSCs were used as controls for marker expression analysis and scratch assays at passage 1. …”
Publicado 2021
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243917por Si Ali, A., Smati-Lafarge, M., Schortgen, F., Vanoli, E., Boudjemaa, A., Varon, E., Maitre, B.“…Un même profil génomique entre les souches patients. 9 parmi 35 contrôles d’air sont RT-PCR positifs (26%). Les cultures virales sont négatives. L’extraction d’air dans les chambres est très faible (6% en 70 secondes), vu le faible taux de renouvellement d’air = 2,8 volumes/heure. …”
Publicado 2022
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243918por Chen, Xuxiang, Zhou, Changqing, Xu, Daishi, Liu, Xin, Li, Shuangmei, Hou, Jingyu, Zhang, Kanglong, Zeng, Chaotao, Zheng, Guanghui, Wu, Haidong, Wu, Hao, Wang, Wuming, Fu, Jiaying, Wang, Tong“…RAT BM-MSCs of the third passage were divided into control group, hypoxic/ischemic (H/I) group, ELA group, ELA-LY group and LY group. RAT BM-MSCs were cultured under normoxia in control group. In H/I group, RAT BM-MSCs were exposed to hypoxia (1% O2) and serum deprivation for 24 h. …”
Publicado 2022
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243919por Landman, Anadeijda J. E. M. C., de Boer, Marjon A., Visser, Laura, Nijman, Tobias A. J., Hemels, Marieke A. C., Naaktgeboren, Christiana N., van der Weide, Marijke C., Mol, Ben W., van Laar, Judith O. E. H., Papatsonis, Dimitri N. M., Bekker, Mireille N., van Drongelen, Joris, van Pampus, Mariëlle G., Sueters, Marieke, van der Ham, David P., Sikkema, J. Marko, Zwart, Joost J., Huisjes, Anjoke J. M., van Huizen, Marloes E., Kleiverda, Gunilla, Boon, Janine, Franssen, Maureen T. M., Hermes, Wietske, Visser, Harry, de Groot, Christianne J. M., Oudijk, Martijn A.“…Secondary outcomes included a composite of poor neonatal outcome (bronchopulmonary dysplasia, periventricular leukomalacia > grade 1, intraventricular hemorrhage > grade 2, necrotising enterocolitis > stage 1, retinopathy of prematurity, culture proven sepsis, or perinatal death). Analyses were performed by intention to treat. …”
Publicado 2022
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243920por Huang, Chaoqun, Liu, Jiuyang, He, Liang, Wang, Fubing, Xiong, Bin, Li, Yan, Yang, Xiaojun“…Real-time reverse transcription-polymerase chain reaction (RT-PCR) was adopted to gauge the expression of NORAD, miR-496, and IL-33 in GC tissues and cells, and gain- or loss-of-function assays were conducted to investigate the role of them in GC. A GC cell-CAFs co-culture model was established to explore the interaction between CAFs and GCs. …”
Publicado 2021
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