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821por Nozari, Shagayegh, Adine, Mohtaram, Javadi, Farzaneh, Shahnazi, Mojtaba, Azadmehr, Abbas, Nassiri-Asl, Marjan, Jahanihashemi, Hasan, Saraei, Mehrzad“…RESULTS: In the cell-free in vitro study, all of tachyzoites were killed at concentrations of 100 mg/mL of both extracts while at concentration 10 mg/mL, the mortality was 4.53% − 5.31%. In the cell culture study, the values of the effective concentration (EC(50)) were 215 and 153 μg/mL and the selectivities were 0.73 and 0.69 for the A. millefolium and the H. perforatum extracts, respectively. …”
Publicado 2016
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822“…Mutations in lamins cause defective organogenesis in mouse models and human diseases that affect adipose tissue, brain, skeletal muscle, and the heart. In vitro cell culture studies have shown that lamins help maintain nuclear shape and facilitate cell migration. …”
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823“…Exosporium tiliae, Corynespora caespitosa, C. endiandrae, C. leucadendri and C. olivacea are recognised in Helminthosporium, and Splanchnonema quercicola and S. kalakadense are combined in Helminthosporium. Based on pure culture studies and DNA sequence data, Massaria heterospora and Massarinula italica are shown to be the sexual morphs of Helminthosporium tiliae and H. microsorum, respectively. …”
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824“…There is a need for functional in vitro 3D human intestine systems that can bridge the gap between conventional cell culture studies and human trials. The successful engineering in vitro of human intestinal tissues relies on the use of the appropriate cell sources, biomimetic scaffolds, and 3D culture conditions to support vital organ functions. …”
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825“…Ethanol (50 mM) decreased viability and ATP content and increased mitochondrial mass, while NNK (100 μM or higher) selectively inhibited mitochondrial function. The slice culture studies demonstrated striking adverse effects of ethanol, NNK and ethanol+NNK exposures manifested by architectural disorganization of the cortex with relative reductions of internal granule cells, increases in external granule cells, and loss of Purkinje cells. …”
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826por Lu, Chien-Lin, Shyu, Jia-Fwu, Wu, Chia-Chao, Hung, Chi-Feng, Liao, Min-Tser, Liu, Wen-Chih, Zheng, Cai-Mei, Hou, Yi-Chou, Lin, Yuh-Feng, Lu, Kuo-Cheng“…Then, we explored the source of Wnt 10b secretion by using osteoclasts and osteoblasts treated with calcitriol in cell culture studies. Finally, we explored the effects of calcitriol on bone microarchitectures in CKD mice, using the 5/6 nephrectomy CKD animal model with analysis using micro-computed tomography. …”
Publicado 2018
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827“…However, G. procumbens did not show a significant effect on insulin level either in the in vivo test or the in vitro RIN-5F cell culture study. G. procumbens also showed minimal effects on β-cells of the islets of Langerhans in the pancreas. …”
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828por Moretti, Daniela, DiFrancesco, Mattia Lorenzo, Sharma, Parikshit Pratim, Dante, Silvia, Albisetti, Edoardo, Monticelli, Marco, Bertacco, Riccardo, Petti, Daniela, Baldelli, Pietro, Benfenati, Fabio“…In this study, we present the first biomagnetic chip based on magnetic tunnel junction (MTJ) technology for cell culture studies and show the biocompatibility of these sensors. …”
Publicado 2018
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829“…Previous reports based on cell culture studies by us and others showed that mutation of Smad3 linker phosphorylation sites dramatically intensifies TGF‐β responses as well as growth‐inhibitory function and epithelial‐mesenchymal transition (EMT), suggesting that Smad3 linker phosphorylation suppresses TGF‐β transcriptional activities. …”
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830por Ercolano, Giuseppe, De Cicco, Paola, Frecentese, Francesco, Saccone, Irene, Corvino, Angela, Giordano, Flavia, Magli, Elisa, Fiorino, Ferdinando, Severino, Beatrice, Calderone, Vincenzo, Citi, Valentina, Cirino, Giuseppe, Ianaro, Angela“…In particular, we investigated the effect of naproxen-HBTA in vitro on several metastatic features of human melanoma cells such as proliferation, migration, invasion, and colonies formation and in vivo in a model of cutaneous melanoma. Cell culture studies demonstrated that naproxen-HBTA induced caspase 3-mediated apoptosis and inhibited motility, invasiveness, and focus formation. …”
Publicado 2019
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831“…Further, in a battery of cell culture studies, we confirmed that PLGA nanoparticles encapsulated nuclear localization sequence/antimiR-155 PS combination undergoes significant intracellular delivery and results in reduced expression of miR-155. …”
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832por Koehler, Miriam I., Hartmann, Eliza S., Schluessel, Sabine, Beck, Felicitas, Redeker, Julia I., Schmitt, Baerbel, Unger, Marina, van Griensven, Martijn, Summer, Burkhard, Fottner, Andreas, Mayer-Wagner, Susanne“…Co-culture studies investigating the role of periprosthetic fibroblasts (PPFs) in inflammatory osteoclastogenesis reveal contrary results, partly showing an osteoprotective function of fibroblasts and high OPG expression in monolayer. …”
Publicado 2019
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833por Mizukami, Takuya, Iso, Yoshitaka, Sato, Chisato, Sasai, Masahiro, Spees, Jeffery L., Toyoda, Masashi, Umezawa, Akihiro, Miyazaki, Akira, Suzuki, Hiroshi“…METHODS AND RESULTS: MSCs were isolated from BM in GFP-transgenic rats. In a culture study, Epo promoted BMMSC proliferation in normoxia and enhanced cell survival under the culture condition mimicking ischemia (1% oxygen and nutrient deprivation). …”
Publicado 2016
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834por Schulz, André, Gepp, Michael M., Stracke, Frank, von Briesen, Hagen, Neubauer, Julia C., Zimmermann, Heiko“…We further demonstrated an enhanced protein adsorption with increasing tyramine conjugation, stable for several weeks. Cell culture studies with human mesenchymal stem cells and human pluripotent stem cell‐derived cardiomyocytes qualified tyramine‐alginate hydrogels as bioactive platforms enabling cell adhesion and contraction on (structured) 2‐D layer and spherical matrices. …”
Publicado 2018
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835por Novikova, Olga A., Nazarkina, Zhanna K., Cherepanova, Anna V., Laktionov, Petr P., Chelobanov, Boris P., Murashov, Ivan S., Deev, Roman V., Pokushalov, Evgeny A., Karpenko, Andrey A., Laktionov, Pavel P.“…Differential expression of several notable transcripts in cells from stable and vulnerable plaques suggests the value of plaque cell culture studies for the search of plaque vulnerability markers.…”
Publicado 2019
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836por Yao, Minghao, Zhang, Junni, Gao, Feng, Chen, Yihao, Ma, Shanshan, Zhang, Kun, Liu, Hongtao, Guan, Fangxia“…This hydrogel possesses a quick gelation process within 5 min, a high water content, and a uniform three-dimensional (3D) porous network. The 3D cell culture study indicated that gelatin hydrogel matrix of HRP(5U):GalOx(1U) or HRP(2U):GalOx(1U) could provide a friendly 3D microenvironment for supporting the survival, proliferation, and spread of mouse bone mesenchymal stem cells (BMSC) with negligible cytotoxicity. …”
Publicado 2019
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837“…Thematic analysis of the data was used to identify themes and patterns from the participants’ perspectives and experiences in the multilingual research context. In this cross-cultural study, for Māori participants, whanaungatanga (family relationships) was recognised as a core value that places whanau (family) at the centre of whakapapa (human and non-human relations). …”
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838por Huang, Sheng-Fu, Othman, Alaa, Koshkin, Alexey, Fischer, Sabrina, Fischer, David, Zamboni, Nicola, Ono, Katsuhiko, Sawa, Tomohiro, Ogunshola, Omolara O.“…In correlation, co-culture studies revealed that blocking AC GSH generation and secretion via siRNA-mediated γ-glutamyl cysteine ligase (GCL) knockdown significantly compromises EC barrier integrity. …”
Publicado 2020
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839por Solanki, Ashish K., Kondkar, Altaf A., Fogerty, Joseph, Su, Yanhui, Kim, Seok-Hyung, Lipschutz, Joshua H., Nihalani, Deepak, Perkins, Brian D., Lobo, Glenn P.“…As for STRA6, modeling and docking studies confirmed three conserved RBP4 binding residues in zebrafish Rbpr2. In cell culture studies, disruption of the RBP4 binding residues on Rbpr2 almost completely abolished uptake of exogenous vitamin A. …”
Publicado 2020
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840por Pritchard, Alexandra, Tousif, Sultan, Wang, Yong, Hough, Kenneth, Khan, Saad, Strenkowski, John, Chacko, Balu K., Darley-Usmar, Victor M., Deshane, Jessy S.“…The concentration of exosomes produced by p53 null H358 lung tumor cells was significantly reduced compared to A549 (p53 wild-type) lung tumor cells, consistent with p53-mediated regulation of exosome production. In co-culture studies, M0 macrophages internalized tumor-derived exosomes, and differentiated into M2 phenotype. …”
Publicado 2020
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