“…Patient records were reviewed electronically for preexisting comorbidities, overall health status, synovial fluid cultures, inflammatory serologies, revision surgeries, and recurrent or persistent infection based on the modified Musculoskeletal Infection Society criteria. …”
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“…A total of 200 patients’ ear discharge and debris collected samples were examined by direct examination and cultured on SDA supplemented with chloramphenicol. …”
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“…Hence, a preliminary diagnosis of disseminated blastomycosis was made. Cultures were put in SDA at 25°C and 37°C and after a week, colonies appeared as fluffy white glabrous with reverse cream-colored non-sporulating colony. …”
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“…Conidia of molds and colonies of yeasts were harvested from fungal cultures on SDA incubated at 35°C; the turbidity of the suspension was then adjusted to OD630 nm = 80%-82%T for molds and 75%-77%T for yeasts. …”
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“…In addition, some of the co-cultures were previously treated with specific blocking antibodies for TLR2 and TLR4 or with a blocking peptide specific for Dectin-1 (CLEC7A). …”
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“…METHODS: A total of 720 samples were screened for C. auris, including clinical samples from patients (tissue, body fluids), surveillance samples from patients (axillar/groin swabs), swabs and water samples from environmental locations and objects, surface swabs from hospital locations, and screening samples from healthcare personnel for hand carriage of C. auris. Samples were cultured on Sabouraud Dextrose agar (SDA) and CHROMagar. …”
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“…Echocardiogram and blood cultures were negative. She was recently started on rituximab, alongside methotrexate. …”
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“…DNA was extracted from isolates cultured on Sabouraud dextrose agar using a commercially available kit (NucleoSpin® Tissue, Macherey-Nagel, Düren, Germany). …”
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“…The presence of Candida spp. was investigated in fecal, gastric fluid, and blow samples from each dolphin twice.  : Samples were cultured on Sabouraud Dextrose Agar and ChromAgar Candida at 30°C for 5 days. …”
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“…Cellular oxidative stress, caspase 3/7 activity, nuclear condensation, lysosomal activity, and DNA synthesis induced by F-UBA in blood cell cultures and oral epithelial squamous cell carcinoma (CLS-354) cell line were investigated through complex flow cytometry analyses. …”
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“…This seasonal phenology continues to be expressed in human-modified landscapes and, while suppressed, it also partially expresses in long-term cultures. We subsequently demonstrate that B. dorsalis, across both its endemic and invasive ranges, shows a very similar seasonality although reversed in the northern hemisphere. …”
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“…The purified cells were cultured to confluence, treated with or without tamoxifen to induce CRE expression, then treated with differentiation cocktail. …”
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“…To this end, primary hypothalamic astrocyte cultures were treated with palmitic acid (PA; 0.5 mM), oleic acid (OA; 0.5 mM) or vehicle for 24 hours and exosomes were isolated from the media and applied (1.25 or 2.50 µg/mL) to a POMC neuronal cell line for 24 hours. …”
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“…Given it was recently demonstrated that DRD2 mediates a reduction of p-AKT in a subset of NF-PitNETs and in rat prolactin-secreting tumor cells MMQ through a β-arrestin 2-dependent mechanism, the aim of the present work was to test the efficacy of adjuvant treatment with cabergoline in increasing sensitivity of human primary cultured NF-PitNET cells to antiproliferative effects of everolimus. …”
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“…We measured de novo fatty acid synthesis in cultured patient and control fibroblast cells with 13C-Glucose, with and without the C75 fatty acid synthase inhibitor. …”
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“…Thus, the aim of this study is to investigate how bisphenols affect granulosa cell viability and through which apoptotic pathway. In vitro cultured granulosa cells were exposed for 12 or 48 hrs to six treatment groups: control, vehicle, estradiol, BPA, BPS and BPF at a low dose (0.005 mg/mL) and at the BPA Lowest Observed Adverse Effect Level (LOAEL-0.05 mg/mL). …”
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“…We further hypothesized that androgens would downregulate insulin signaling pathway components in male vWAT adipocytes dependent on E(2) levels. vWAT and subcutaneous white adipose tissue (scWAT) were collected from male and female rats, digested in sterile-filtered collagenase, and the resulting preadipocytes were proliferated to 80% confluence. After cell cultures were induced to a minimum of 60% adipocyte cells, cells were exposed to the following treatments: no steroid, 5a-dihydrotestosterone (DHT), E(2), Test, fulvestrant, Test + letrozole, and Test + fulvestrant for 48 hours. …”
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“…Then, HSP70 knockdown in MDA-MB-231 cells were performed using siRNA. Macrophages cultured with HSP70 suppressed conditioned media from MDA-MB-231 cells showed decreased expression of CD163, a marker of pro-tumorigenic macrophages. …”
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“…The media bags were off–loaded after one day and allowed to further cool for another day before inoculation. The cultured spawn was used to inoculate the media upon cooling at room temperature. …”
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“…The primary chondrocytes were co-cultured with different phenotypes of macrophages to observe the expression of collagen II and catabolic enzymes in chondrocytes. iPSC-EVs were injected intraarticularly into the rabbit with an ACLT-induced OA model. …”
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