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  1. 12761
    “…We retrieved the complete 54.2 kb microcystin (mcy) gene cluster from a draft genome assembly. Analysis of the substrate specificity of McyB(1) and McyC adenylation domain binding pockets revealed divergent substrate specificity sequences, which could explain the activation of homo-amino acids which were present in 31% of the microcystins detected and included variants such as MC-LHty, MC-HphHty, MC-LHph and MC-HphHph. …”
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    The Biosynthesis of Rare Homo-Amino Acid Containing Variants of Microcystin by a Benthic Cyanobacterium
  2. 12762
    “…At a national workshop, 22 infection experts reached a consensus to define appropriate prescribing and agree upon an initial draft audit tool. Following this, a national multidisciplinary panel of 19 infection experts, of whom only one was part of the workshop, was convened to evaluate and validate variables using questionnaires to confirm the relevance of each variable in assessing appropriate prescribing. …”
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    Measuring Appropriate Antibiotic Prescribing in Acute Hospitals: Development of a National Audit Tool Through a Delphi Consensus
  3. 12763
    “…Here, we for the first time sequenced and assembled a draft genome for a seawater cultured blue tilapia (0.92 Gb), with 97.8% completeness and a scaffold N50 of 1.1 Mb, which suggests a relatively high quality of this genome assembly. …”
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    Whole Genome Sequencing of the Blue Tilapia (Oreochromis aureus) Provides a Valuable Genetic Resource for Biomedical Research on Tilapias
  4. 12764
    “…The translation (English → Portuguese) and back-translation (Portuguese → English) were both carried out by translators, working independently, who were native speakers of one language and fluent in the other. After review, a draft version was created for pilot testing in lung cancer patients in Brazil. …”
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    Translation and cultural adaptation of the European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire-Lung Cancer Module for quality of life assessment in patients with lung cancer in Brazil
  5. 12765
    “…Frequently, activities performed in Spain (n = 103) were listed in an initial draft. The Likert scale was administrated to a large group of Spanish nationals over the age of 60 years (n = 98) to establish which type of activities will be eventually included in the Spanish version. …”
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    Spanish Transcultural Adaptation of the Activity Card Sort
  6. 12766
    “…With the release of the draft genome of the grass carp, researches on the grass carp from the genetic level and the further molecular mechanisms of economically valuable physiological behaviors have gained great attention. …”
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    The Grass Carp Genomic Visualization Database (GCGVD): an informational platform for genome biology of grass carp
  7. 12767
    “…Here, we reconstructed a draft genome of USCα from a combination of targeted cell sorting and metagenomes from forest soil, providing the first insights into its metabolic potential and environmental adaptation strategies. …”
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    Unravelling the Identity, Metabolic Potential and Global Biogeography of the Atmospheric Methane‐Oxidizing Upland Soil Cluster α
  8. 12768
    “…An iterative process involving six sequential stages was conducted to develop a set of draft true/false items. These were then administered to 539 target‐age school pupils (mean 12.8; SD ± 1.3), from the United Kingdom. …”
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    Development of a measure of genome sequencing knowledge for young people: The kids‐KOGS
  9. 12769
    “…This new assembly represents approximately 96% of the non-repetitive and 84% of the entire bobwhite genome size, greatly improves scaffold lengths and contiguity compared to an existing draft bobwhite genome, and provides an important tool for future studies of evolutionary and functional genomics in bobwhites.…”
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    A Highly Contiguous Reference Genome for Northern Bobwhite (Colinus virginianus)
  10. 12770
    “…Yet, few anuran genomes have been sequenced, limiting research on these organisms. Here, we produce a draft genome for the Mexican spadefoot toad, Spea multiplicata, which is a member of an unsequenced anuran clade. …”
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    Genome of Spea multiplicata, a Rapidly Developing, Phenotypically Plastic, and Desert-Adapted Spadefoot Toad
  11. 12771
    “…Here, we report the results of the sequencing and analysis of the high-quality draft genome of the thermophilic chemolithoautotrophic anaerobe Deferribacter autotrophicus. …”
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    Genomic Insights into the Carbon and Energy Metabolism of a Thermophilic Deep-Sea Bacterium Deferribacter autotrophicus Revealed New Metabolic Traits in the Phylum Deferribacteres
  12. 12772
    “…For the format of the draft recommendations, an adapted version of the Appraisal of Guidelines for Research and Evaluation II tool was used. …”
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    Infection control recommendations for radiology departments in Malawi
  13. 12773
    “…We e-mailed these researchers to ask them to draft a brief summary of the main function(s) of the gene’s product, which we edited for consistency to produce a ‘gene snapshot’. …”
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    Building a pipeline to solicit expert knowledge from the community to aid gene summary curation
  14. 12774
    “…Here, we assemble a high-quality genome for D. albomicans using single-molecule sequencing and chromatin conformation capture, and draft genomes for 11 additional species and 67 individuals across the clade, to infer the species phylogeny and patterns of genetic diversity in this group. …”
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    Patterns of Genomic Differentiation in the Drosophila nasuta Species Complex
  15. 12775
    “…To facilitate the construction of standardized and robust phylogenies for disparate types of projects, we have developed a complete bioinformatic workflow, with a web-based component to perform phylogenetic and molecular evolutionary (PhaME) analysis from sequencing reads, draft assemblies or completed genomes of closely related organisms. …”
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    Standardized phylogenetic and molecular evolutionary analysis applied to species across the microbial tree of life
  16. 12776
    “…EFSA prepared a conclusion where the assessment of the information is presented according to the draft technical guidance on assessment of negligible exposure of an active substance in a plant protection product under realistic conditions of use. …”
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    Peer review of the pesticide risk assessment for the active substance isoxaflutole in light of negligible exposure data submitted
  17. 12777
    “…Various measures have been taken to mitigate this issue by adjusting the environment; however, the cellular processes of Pacific abalone (Haliotis discus hannai) have been overlooked due to the paucity of genetic information. The draft genome of H. discus hannai has recently been reported, prompting exploration of the genes responsible for thermal regulation in Pacific abalone. …”
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    Expression of Heat Shock Proteins in Thermally Challenged Pacific Abalone Haliotis discus hannai
  18. 12778
    “…To explore the genetic diversity and potential niche-specific adaptation changes of L. ruminis, in the current work, draft genomes of 81 L. ruminis strains isolated from human, bovine, piglet, and other animals were sequenced, and comparative genomic analysis was performed. …”
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    Comparative Genomics Analysis of Lactobacillus ruminis from Different Niches
  19. 12779
    “…Our knowledge of cat family biology was recently expanded to include a genomics perspective with the completion of a draft whole genome sequence of an Abyssinian cat. …”
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    State of cat genomics
  20. 12780
    “…In a detailed analysis of the habu draft genome, we found a gene encoding a novel SSP, SSP-6. …”
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    Discovery of the Gene Encoding a Novel Small Serum Protein (SSP) of Protobothrops flavoviridis and the Evolution of SSPs
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