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  1. 4821
    “…Experimentally, host-microbiota interactions are investigated by working with animals devoid of symbiotic bacteria, i.e., by the decimation of gut commensals by antibiotic administration, or by taking advantage of germ-free mouse isolator technology. Remarkably, some of the vascular effects that were unraveled following antibiotic treatment were not observed in the germ-free animal models and vice versa. …”
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    Antibiotic Treatment Protocols and Germ-Free Mouse Models in Vascular Research
  2. 4822
    “…This strategy consisted of a constitutive and methanol-free enzyme production step coupled with a two-step filtration process. …”
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    Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris
  3. 4823
    “…A graphene field-effect transistor (gFET) was non-covalently functionalised with 1-pyrenebutyric acid N-hydroxysuccinimide ester and conjugated with anti-CD63 antibodies for the label-free detection of exosomes. Using a microfluidic channel, part of a graphene film was exposed to solution. …”
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    Chemically Functionalised Graphene FET Biosensor for the Label-free Sensing of Exosomes
  4. 4824
    por Filipe, Joao A.N., Kyriazakis, Ilias
    Publicado 2019
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    Bayesian, Likelihood-Free Modelling of Phenotypic Plasticity and Variability in Individuals and Populations
  5. 4825
    “…Multiphoton microscopy (MPM) is a label-free imaging technique that can produce intraoperative histological images of fresh, unprocessed surgical specimens. …”
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    Rapid, label‐free detection of intracranial germinoma using multiphoton microscopy
  6. 4826
    “…The quantitative determination of immunoglobulin free light chains (FLCs) is considered to be the gold standard in the detection and treatment of multiple myeloma (MM) and amyloid light-chain (AL) amyloidosis. …”
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    Silver Nanoparticle-Based Assay for the Detection of Immunoglobulin Free Light Chains
  7. 4827
    “…Knowledge of hydration properties of gluten‐free ingredients can contribute to improve the quality of gluten‐free products. …”
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    Physicochemical and hydration properties of different cereal and legume gluten‐free powders
  8. 4828
    “…We present a novel label‐free approach using an imaging flow cytometer and machine learning algorithms, where live, unstained WBCs were classified. …”
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    Label‐Free Identification of White Blood Cells Using Machine Learning
  9. 4829
    “…Type 2 diabetes mellitus (T2DM) is associated with increased total plasma free fatty acid (FFA) concentrations and an elevated risk of cardiovascular disease. …”
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    Changes in Plasma Free Fatty Acids Associated with Type-2 Diabetes
  10. 4830
    “…Free from the limitations posed by exogenous scaffolds or extracellular matrix-based materials, scaffold-free engineered tissues have immense clinical potential. …”
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    Scaffold-Free Bioprinter Utilizing Layer-By-Layer Printing of Cellular Spheroids
  11. 4831
    “…Immunofluorescence and western blotting demonstrated PUUV-IgG and -IgA in urine, however, the presence of intact immunoglobulins did not fully explain the LFRET signals. We purified free light chains (FLCs) from both urine and serum of healthy volunteers and patients with acute PUUV infection, and verified the presence of antigen-specific FLCs. …”
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    Urine and Free Immunoglobulin Light Chains as Analytes for Serodiagnosis of Hantavirus Infection
  12. 4832
    “…INTRODUCTION: Our aim was to compare analytical specifications of two assays (monoclonal vs. polyclonal) for free light chains (FLCs) quantification optimized for two different analytical platforms, nephelometer ProSpec (Siemens, Erlangen, Germany) and turbidimetric analyser Optilite (The Binding Site, Birmingham, UK). …”
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    Verification study of free light chains assays on reagent-optimized analysers
  13. 4833
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    Free Flap Skin Paddle: A Painless Donor Site In Vivo
  14. 4834
    “…Molecular dynamics simulations provide the primary computational technique to estimate the free energies of these interactions. We demonstrate that the energetics of protein–lipid interactions may be reliably and reproducibly calculated using three simulation-based approaches: potential of mean force calculations, alchemical free energy perturbation, and well-tempered metadynamics. …”
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    Insights into Membrane Protein–Lipid Interactions from Free Energy Calculations
  15. 4835
    “…By studying the reaction profile, in particular the selectivity for the formation of mono- and bis-condensation products, a high yield of the desired product can be obtained through an operationally simple and solvent-free protocol.…”
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    Solvent-Free Synthesis of 2,5-Bis((dimethylamino)methylene)cyclopentanone
  16. 4836
    “…To overcome these limitations, here we report development of the DSB-free, single-nucleotide–resolution genome editing system CRISPR-BEST (CRISPR-Base Editing SysTem), which comprises a cytidine (CRISPR-cBEST) and an adenosine (CRISPR-aBEST) deaminase-based base editor. …”
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    Highly efficient DSB-free base editing for streptomycetes with CRISPR-BEST
  17. 4837
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    Label-Free Telomerase Activity Detection via Electrochemical Impedance Spectroscopy
  18. 4838
    “…This study aimed to determine whether free thyroxine (FT4) influences the prevalence of prediabetes in euthyroid subjects using a cross-sectional survey derived from the Korea National Health and Nutrition Examination Survey, conducted between 2013 and 2015. …”
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    Low-Normal Free Thyroxine Levels in Euthyroid Male Are Associated with Prediabetes
  19. 4839
    “…Twenty participants, randomly assigned to two different groups, practiced basketball free throws for three consecutive days, performing 100 throws per day. …”
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    Gaze Behavior in Basketball Free Throws Developed in Constant and Variable Practice
  20. 4840
    “…The most recent eradication plan provides more stringent measures to combat free‐ranging pigs and any kind of illegality in the pig sector. …”
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    Surveillance and control of African Swine Fever in free‐ranging pigs in Sardinia
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