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  1. 5001
    “…Optimization of the GA metabolic pathways in host cells has leapt forward, and the titer and yield have increased rapidly. Meanwhile, cell-free multi-enzyme catalysis, in which the desired pathway is constructed in vitro from enzymes and cofactors involved in GA biosynthesis, has also realized efficient GA bioconversion. …”
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    Cell-based and cell-free biocatalysis for the production of d-glucaric acid
  2. 5002
    “…Unfortunately, cell-free systems can require specialized DNA architectures with minimal regulation that are not compatible with cellular expression. …”
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    Modular cell-free expression plasmids to accelerate biological design in cells
  3. 5003
    “…In this study, we present our experience using the free anterolateral thigh (ALT) flap in the reconstruction of extensive defects of plantar forefoot. …”
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    Reconstruction of extensive plantar forefoot defects with free anterolateral thigh flap
  4. 5004
    “…Starting January, PHNs at A city (population approx. 210,000) established a free telephone consultation hotline for COVID-19. …”
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    COVID-19-Related Free Telephone Consultations by Public Health Nurses
  5. 5005
    “…In 1991 and 2011, shorter total and disability-free years were associated with greater multi-morbidity. …”
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    Multimorbidity, Disadvantage, and Trends in Disability-Free Life Expectancy: The CFAS Studies
  6. 5006
    por Jagger, Carol
    Publicado 2020
    “…Life expectancy has increased over previous decades, but several countries are seeing widening inequalities in disability-free life expectancy (DFLE) by socioeconomic position (SEP). …”
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    Why Are Inequalities in Disability-Free Life Expectancy by Socioeconomic Position Widening?
  7. 5007
    “…In particular, using Freedom House’s coding and terminological classifications, the proportion of often illicit Onion/Hidden Services use is more prevalent (∼7.8%) in “free” countries than in either “partially free” (∼6.7%) or “not free” regimes (∼4.8%).…”
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    The potential harms of the Tor anonymity network cluster disproportionately in free countries
  8. 5008
    “…Impedance flow cytometry (IFC) is a versatile lab-on-chip technology which enables fast and label-free analysis of pollen grains in various plant species, promising new research possibilities in agriculture and plant breeding. …”
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    Hazelnut Pollen Phenotyping Using Label-Free Impedance Flow Cytometry
  9. 5009
    por Kuo, Heng-Wei, Pan, Kuo-Long, Lee, Wei-Li
    Publicado 2020
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    A propellant-free superconducting solenoid thruster driven by geomagnetic field
  10. 5010
    “…Towards this goal we revisit the ideal free distribution (IFD), where consumers are assumed to be omniscient, equally competitive and freely moving, and are hence expected to instantaneously distribute themselves across a heterogeneous landscape such that fitness is equalised across the population. …”
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    Habitat selection patterns are density dependent under the ideal free distribution
  11. 5011
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    Affinity Purification of Label-free Tubulins from Xenopus Egg Extracts
  12. 5012
    “…Here, we describe two methods that visualize microtubule dynamics at high speed and high contrast: (1) total internal reflection fluorescence microscopy and (2) label-free interference reflection microscopy. For complete details on the use and execution of this protocol, please refer to Hirst et al. (2020).…”
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    In Vitro Reconstitution and Imaging of Microtubule Dynamics by Fluorescence and Label-free Microscopy
  13. 5013
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    NMR microsystem for label-free characterization of 3D nanoliter microtissues
  14. 5014
    “…In order to achieve label-free detection with the sub-100 nm-thick lipid multilayer grating lines, the binding affinity was improved by varying the lipid composition. …”
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    Aptamer Functionalized Lipid Multilayer Gratings for Label-Free Analyte Detection
  15. 5015
    “…A recent focus of research for early detection is the use of circulating cell-free tumour DNA (ctDNA). The detection of ctDNA offers potential as a relatively non-invasive method of diagnosing pancreatic cancer by using genetic sequencing technology to detect tumour-specific mutational signatures in blood samples before symptoms manifest. …”
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    Circulating Cell-Free Tumour DNA for Early Detection of Pancreatic Cancer
  16. 5016
    por Daniell, Henry
    Publicado 2020
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    PBJ ranks higher, enhances diversity and offers free global access
  17. 5017
    “…This review summarizes the progress made toward such development, the open challenges existing, and the approaches undertaken to generate carbon-free energy through water splitting. [Image: see text]…”
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    Recent Progress and Approaches on Carbon-Free Energy from Water Splitting
  18. 5018
    “…Free and partially encapsulated manganese ferrite (MnFe(2)O(4)) nanoparticles are synthesized and characterized regarding structure, surface, and electronic and magnetic properties. …”
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    Free and partially encapsulated manganese ferrite nanoparticles in multiwall carbon nanotubes
  19. 5019
    “…The flap’s added bulk and its minimal scar retraction granted free tongue movement and optimal speech intelligibility.…”
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    Tip of the Tongue Reconstruction with Prelaminated Fasciomucosal Radial Forearm Free Flap
  20. 5020
    “…However, the realization that the beneficial effect of MSCs relies mainly on their paracrine action, rather than on their engraftment in the recipient tissue and subsequent differentiation, has opened the way to cell-free therapeutic strategies in regenerative medicine. …”
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    Mesenchymal stem cells secretome: The cornerstone of cell-free regenerative medicine
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