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  1. 6001
    por Aquila, Andrew, Hunter, Mark S., Doak, R. Bruce, Kirian, Richard A., Fromme, Petra, White, Thomas A., Andreasson, Jakob, Arnlund, David, Bajt, Saša, Barends, Thomas R. M., Barthelmess, Miriam, Bogan, Michael J., Bostedt, Christoph, Bottin, Hervé, Bozek, John D., Caleman, Carl, Coppola, Nicola, Davidsson, Jan, DePonte, Daniel P., Elser, Veit, Epp, Sascha W., Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Frank, Matthias, Fromme, Raimund, Graafsma, Heinz, Grotjohann, Ingo, Gumprecht, Lars, Hajdu, Janos, Hampton, Christina Y., Hartmann, Andreas, Hartmann, Robert, Hau-Riege, Stefan, Hauser, Günter, Hirsemann, Helmut, Holl, Peter, Holton, James M., Hömke, André, Johansson, Linda, Kimmel, Nils, Kassemeyer, Stephan, Krasniqi, Faton, Kühnel, Kai-Uwe, Liang, Mengning, Lomb, Lukas, Malmerberg, Erik, Marchesini, Stefano, Martin, Andrew V., Maia, Filipe R.N.C., Messerschmidt, Marc, Nass, Karol, Reich, Christian, Neutze, Richard, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Schlichting, Ilme, Schmidt, Carlo, Schmidt, Kevin E., Schulz, Joachim, Seibert, M. Marvin, Shoeman, Robert L., Sierra, Raymond, Soltau, Heike, Starodub, Dmitri, Stellato, Francesco, Stern, Stephan, Strüder, Lothar, Timneanu, Nicusor, Ullrich, Joachim, Wang, Xiaoyu, Williams, Garth J., Weidenspointner, Georg, Weierstall, Uwe, Wunderer, Cornelia, Barty, Anton, Spence, John C. H., Chapman, Henry N.
    Publicado 2012
    “…We demonstrate the use of an X-ray free electron laser synchronized with an optical pump laser to obtain X-ray diffraction snapshots from the photoactivated states of large membrane protein complexes in the form of nanocrystals flowing in a liquid jet. …”
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    Time-resolved protein nanocrystallography using an X-ray free-electron laser
  2. 6002
    “…We have developed a label-free quantitative SRM workflow that relies on a new automated algorithm, Anubis, for accurate peak detection. …”
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    Automated Selected Reaction Monitoring Software for Accurate Label-Free Protein Quantification
  3. 6003
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    Model-Free Reconstruction of Excitatory Neuronal Connectivity from Calcium Imaging Signals
  4. 6004
    “…The end products of lipid peroxidation are reactive aldehydes such as 4-hydroxy-2-nonenal (HNE), acting as “second messengers of free radicals.” Although reactive aldehydes were first recognized only as cytotoxic, new evidence has come to light, related to their cell growth regulatory functions achieved through cell signaling. …”
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    Even stressed cells are individuals: second messengers of free radicals in pathophysiology of cancer
  5. 6005
    “…We augmented molecular dynamics (MD) with Hamiltonian Replica Exchange and calculated binding free energy changes for H274Y, N294S, and Y252H mutants. …”
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    Quantitative Predictions of Binding Free Energy Changes in Drug-Resistant Influenza Neuraminidase
  6. 6006
    “…We accelerated the mRNA reprogramming process through stepwise optimization of the RF cocktail and leveraged these kinetic gains to establish a feeder-free, xeno-free protocol which slashes the time, cost and effort involved in iPSC derivation.…”
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    Feeder-Free Derivation of Human Induced Pluripotent Stem Cells with Messenger RNA
  7. 6007
    “…LMF may represent a superior technique for the generation of virus-free or integration-free iPS cell lines that could lead to enhanced stem cell therapy in the future.…”
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    Efficient Generation of Virus-Free iPS Cells Using Liposomal Magnetofection
  8. 6008
    “…It requires only 100 ng of DNA for amplification-free WGBS of mammalian genomes. Thus, the PBAT method will enable various novel applications that would not otherwise be possible, thereby contributing to the rapidly growing field of epigenomics.…”
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    Amplification-free whole-genome bisulfite sequencing by post-bisulfite adaptor tagging
  9. 6009
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    Next-generation antithrombotics in ischemic stroke: preclinical perspective on ‘bleeding-free antithrombosis'
  10. 6010
    “…Free-flying honey bees (Apis mellifera L.) reactions were observed when presented with varying schedules of post-reinforcement delays of 0 s, 300 s, or 600 s. …”
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    Social Reinforcement Delays in Free-Flying Honey Bees (Apis mellifera L.)
  11. 6011
    “…Passive and label-free isolation of viable target cells based on intrinsic biophysical cellular properties would allow for cost savings in applications where molecular biomarkers are known as well as potentially enable the separation of cells with little-to-no known molecular biomarkers. …”
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    Label-Free Enrichment of Adrenal Cortical Progenitor Cells Using Inertial Microfluidics
  12. 6012
    “…This study examined whether obesity is characterized by higher 24 h mean pulse pressure (24 h mean SBP-24 h mean DBP) and whether free thyroid hormones (FT(3) and FT(4)) have a relationship with 24 h mean pulse pressure. …”
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    Abdominal Obesity Is Characterized by Higher Pulse Pressure: Possible Role of Free Triiodothyronine
  13. 6013
    “…We have developed a simple electrochemical biosensing strategy for the label-free diagnosis of hepatitis B virus (HBV) on a gold electrode surface. …”
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    Label-Free Electrochemical Diagnosis of Viral Antigens with Genetically Engineered Fusion Protein
  14. 6014
    por Yoon, Won Young, Lee, Byung Il
    Publicado 2012
    “…CONCLUSIONS: When used properly, the free toe tissue transfer without venous anastomosis method can be a treatment option for small defects on the fingertip area.…”
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    Fingertip Reconstruction Using Free Toe Tissue Transfer Without Venous Anastomosis
  15. 6015
    por Moy, FM, Chang, EWH, Kee, KW
    Publicado 2011
    “…Therefore, we aim to determine the associated predictors with handgrip strength among the free living elderly in Malaysia. METHODS: This was a cross sectional study conducted in a rural state in Malaysia. …”
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    Predictors of Handgrip Strength among the Free Living Elderly in Rural Pahang, Malaysia
  16. 6016
    por Park, Hoo Keun, Oh, Ji Hye, Rag Do, Young
    Publicado 2012
    “…Scatter-free phosphors promise to suppress the scattering loss of conventional micro-size powder phosphors in white phosphor-converted light-emitting diodes (pc-LEDs). …”
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    Toward scatter-free phosphors in white phosphor-converted light-emitting diodes
  17. 6017
    “…In sum, the response of mice to a carbohydrate-free diet was greater weight gain and metabolic disruptions in distinction to the response in humans where low carbohydrate diets cause greater weight loss than isocaloric controls. …”
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    Response of C57Bl/6 mice to a carbohydrate-free diet
  18. 6018
    “…BACKGROUND: Tick-borne haemoparasites Babesia vogeli and Anaplasma platys are common among the free-roaming canine populations associated with Aboriginal communities in Australia, whilst the prevalence of haemoplasmas, which are also suspected to be tick-borne, remained unexplored. …”
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    Haemoparasites of free-roaming dogs associated with several remote Aboriginal communities in Australia
  19. 6019
    “…Recent studies predict that the Arctic Ocean will have ice-free summers within the next 30 years. This poses a significant challenge for the marine organisms associated with the Arctic sea ice, such as marine mammals and, not least, the ice-associated crustaceans generally considered to spend their entire life on the underside of the Arctic sea ice. …”
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    Retention of ice-associated amphipods: possible consequences for an ice-free Arctic Ocean
  20. 6020
    “…In the present study, we further revealed that, in chicken HD11 macrophages and primary monocytes, induction of HDPs is largely in an inverse correlation with the aliphatic hydrocarbon chain length of free fatty acids, with SCFAs being the most potent, medium-chain fatty acids moderate and long-chain fatty acids marginal. …”
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    Modulation of Antimicrobial Host Defense Peptide Gene Expression by Free Fatty Acids
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