“…Using this database and gene expression data from NIH3T3 cells (unable to support B cell development), OP-9 cells (strongly supportive of B cell development), pro-B and pre-B cells as well as mature peripheral B-lineage cells, we were able to identify a set of potential stage and stromal cell restricted communication pathways. Functional analysis of some of these potential ways of communication allowed us to identify BMP-4 as a potent stimulator of B-cell development in vitro. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In this study, we report the functional analysis of RAP2.1, a DREB-type transcriptional repressor. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…CONCLUSION: Our results of cloning, sequencing and functional analysis of AmCPV S1 and S3 indicate that S3 encoded viral structural proteins can self assemble to form viral outer capsid and S1 encoded protein remains associated with it as inner capsid to maintain the stability. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Several CEBFs are known to mediate defense or abiotic stress response, but the physiological functions of other CEBFs remain to be determined. Our in vivo functional analysis of several CEBFs suggests that they are likely to be involved in ABA and/or sugar response. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Twenty-one segregation distortion regions (SDRs) were found in this map; three segregation distorted chromosomes, Chr02, Chr16, and Chr18, were identified with 99.9% of distorted markers segregating toward the heterozygous allele. Functional analysis of SSR sequences showed that 1633 loci of this map (70.6%) were transcribed loci and 1332 loci (57.5%) were translated loci. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We have previously shown that the HPV16 E6 oncoprotein binds to the PDZ domain protein Tax-interacting-protein 1 (Tip-1) and we now report identification and functional analysis of a novel Tip-1 binding GEF. METHODS: Yeast two-hybrid, in vitro pull-down, site-directed mutagenesis, semiquantitative PCR, co-immunoprecipitation and western blotting were used to identify/confirm novel Tip-1 binding partners and analyse cellular expression levels. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Rats fed 5 μg Se/g diet had significantly altered expression of 1193 liver transcripts, whereas mice or rats fed ≤ 2 μg Se/g diet had < 10 transcripts significantly altered relative to Se-adequate animals within an experiment. Functional analysis of genes altered by Se toxicity showed enrichment in cell movement/morphogenesis, extracellular matrix, and development/angiogenesis processes. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…These can range from the ex-vivo and in-vitro detection and functional analysis of Hz-containing leukocytes to the detection of parasitized Red-Blood-Cells (pRBCs) to assess antimalarial activity. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We identified 935 differentially expressed genes (469 L3i-biased; 466 L1-biased) having two-fold expression differences or greater and microarray signals with a p value<0.01. Based on a functional analysis, L1 larvae have a larger number of genes putatively involved in transcription (p = 0.004), and L3i larvae have biased expression of putative heat shock proteins (such as hsp-90). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…BACKGROUND: Precise and efficient methods for gene targeting are critical for detailed functional analysis of genomes and regulatory networks and for potentially improving the efficacy and safety of gene therapies. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The research will provide useful clues for further functional analysis of DEV gI gene.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The network was confirmed to be non-random, scale-free, and highly modular. Functional analysis of gene co-expression modules revealed processes including ‘Nervous system development’, ‘Immune response’, and ‘Cell-cycle’. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…CONCLUSIONS: Our results from functional analysis in transgenic Drosophila show that the BhC4-1 core promoter drives gene expression regardless of the promoter context that was assayed. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The functional analysis of missense mutations can be complicated by the presence in the cell of the endogenous protein. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Gene annotation enrichment analysis is a functional analysis technique that has gained widespread attention and for which many tools have been developed. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…As part of this protocol, we also describe methods for analyzing the tissue engineered myocardium after the culture period including functional analysis of the active force generated by the cardiac muscle construct upon electrical stimulation, as well as methods for determining final cell viability (Live-Dead assay) and immunohistological staining to examine the expression and morphology of typical proteins important for contraction (Myosin Heavy Chain or MHC) and cellular coupling (Connexin 43 or Cx43) between myocytes.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Myc upregulation by insulin and TOR pathways is a mechanism conserved in cells from the wing imaginal disc, where expression of Dp110 and Rheb also induces Myc protein accumulation, while inhibition of insulin and TOR pathways result in the opposite effect. Our functional analysis, aimed at quantifying the relative contribution of Myc to ommatidial growth downstream of insulin and TOR pathways, revealed that Myc activity is necessary to sustain the proliferation of cells from the ommatidia upon Dp110 expression, while its contribution downstream of TOR is significant to control the size of the ommatidia. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…CONCLUSIONS: This well-powered primary and replication genetic study, together with functional analysis of gene expression, implicate CXCR2 in determining outcome of VL in India.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…These results provide a useful reference for the cloning of candidate genes from the specific subgroup for further functional analysis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00438-012-0686-8) contains supplementary material, which is available to authorized users.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Glands were submitted to morphological and functional analysis. In thyroids, volumetric ratios between thyrocytes and colloid were measured. cAMP production in 10(−7)M and 10(−8)M thyrotropin-treated samples was studied. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso