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  1. 10381
    “…RESULTS: Although strain RL demonstrated clear species demarcation (ANI ≤ 80.03%) from the rest of its phylogenetic relatives, it was found to be closest to P. stutzeri clade which was further complemented functionally. Comparative functional analysis elucidated strain specific enrichment of metabolic pathways like α-linoleic acid degradation and carbazole degradation in Pseudomonas sp. strain RL and P. stutzeri XLDN-R, respectively. …”
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    Pan-genome dynamics of Pseudomonas gene complements enriched across hexachlorocyclohexane dumpsite
  2. 10382
    “…Total genomic reads were aligned to the KEGG genes databasefor functional analysis. RESULTS: Deep sequencing produced on average 11.3 Gb per sample. 16S rRNA gene abundances indicated that archaea, predominantly Methanobrevibacter, were 2.5× more numerous (P = 0.026) in high emitters, whereas among bacteria Proteobacteria, predominantly Succinivibrionaceae, were 4-fold less abundant (2.7 vs. 11.2 %; P = 0.002). …”
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    The rumen microbial metagenome associated with high methane production in cattle
  3. 10383
    “…CONCLUSIONS: Our results suggest that functional analysis of PI3K/mTOR signaling is a biomarker tool to identify disease-associated molecular defects that could serve as therapeutic targets in autism. …”
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    Increased expression of the PI3K catalytic subunit p110δ underlies elevated S6 phosphorylation and protein synthesis in an individual with autism from a multiplex family
  4. 10384
    “…RNA sequencing was performed on spleen samples from all 64 birds and differential gene expression analysis was performed for four comparisons: L10L line versus L10H line for uninfected birds at weeks 1 and 3, respectively, and in the same way for infected birds. Functional analysis was performed using Gene Ontology (GO) Immune System Process terms specific for Gallus gallus. …”
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    RNA sequencing-based analysis of the spleen transcriptome following infectious bronchitis virus infection of chickens selected for different mannose-binding lectin serum concentrations
  5. 10385
    “…The results of this research would provide useful information for the next-step work including fine mapping, gene functional analysis, pyramiding breeding of functional genes as well as marker-assisted selection. …”
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    Construction of a high-density genetic map by specific locus amplified fragment sequencing (SLAF-seq) and its application to Quantitative Trait Loci (QTL) analysis for boll weight in upland cotton (Gossypium hirsutum.)
  6. 10386
    “…The GWAS revealed 61 QTL for all traits, located on GGA (Gallus gallus chromosome) 1, 3, 6, 9, 10, 12–14, 17, 18, 21–28, and Z. A functional analysis of the genes in these QTL regions identified the Angiopoietin pathway as significant. …”
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    Quantitative trait loci identified for blood chemistry components of an advanced intercross line of chickens under heat stress
  7. 10387
    “…Based on segregation data, functional analysis and software estimations we assumed that three of novel variants were causative, nine likely causative. …”
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    Next-generation sequencing for diagnosis of thoracic aortic aneurysms and dissections: diagnostic yield, novel mutations and genotype phenotype correlations
  8. 10388
    “…The hsa-miR-144, first time identified in CAKUT, could be connected with biological processes crucial for normal development of kidney and urinary tract. Further functional analysis must follow to reveal the impact of hsa-miR-144 on CAKUT occurrence. …”
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    Transcriptome-wide based identification of miRs in congenital anomalies of the kidney and urinary tract (CAKUT) in children: the significant upregulation of tissue miR-144 expression
  9. 10389
    “…The direct comparison among surface marker schemes validated their selection for highly overlapping subsets of cells. Functional analysis in vitro showed no differences in the abilities of cells sorted by these different methods to grow in culture and differentiate. …”
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    Established cell surface markers efficiently isolate highly overlapping populations of skeletal muscle satellite cells by fluorescence-activated cell sorting
  10. 10390
    “…The validated miRNAs were further investigated by functional analysis with Ingenuity Pathway Analysis software. …”
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    Urinary chromium is associated with changes in leukocyte miRNA expression in obese subjects
  11. 10391
    “…Bioinformatics and functional analysis identified that Kruppel-related zinc finger protein AP-2rep (KLF12) is directly targeted by miR-141. …”
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    MicroRNA-141 enhances anoikis resistance in metastatic progression of ovarian cancer through targeting KLF12/Sp1/survivin axis
  12. 10392
    por Nakayama, Akiyoshi, Nakaoka, Hirofumi, Yamamoto, Ken, Sakiyama, Masayuki, Shaukat, Amara, Toyoda, Yu, Okada, Yukinori, Kamatani, Yoichiro, Nakamura, Takahiro, Takada, Tappei, Inoue, Katsuhisa, Yasujima, Tomoya, Yuasa, Hiroaki, Shirahama, Yuko, Nakashima, Hiroshi, Shimizu, Seiko, Higashino, Toshihide, Kawamura, Yusuke, Ogata, Hiraku, Kawaguchi, Makoto, Ohkawa, Yasuyuki, Danjoh, Inaho, Tokumasu, Atsumi, Ooyama, Keiko, Ito, Toshimitsu, Kondo, Takaaki, Wakai, Kenji, Stiburkova, Blanka, Pavelka, Karel, Stamp, Lisa K, Dalbeth, Nicola, Sakurai, Yutaka, Suzuki, Hiroshi, Hosoyamada, Makoto, Fujimori, Shin, Yokoo, Takashi, Hosoya, Tatsuo, Inoue, Ituro, Takahashi, Atsushi, Kubo, Michiaki, Ooyama, Hiroshi, Shimizu, Toru, Ichida, Kimiyoshi, Shinomiya, Nariyoshi, Merriman, Tony R, Matsuo, Hirotaka
    Publicado 2017
    “…While NIPAL1 encodes a magnesium transporter, functional analysis did not detect urate transport via NIPAL1, suggesting an indirect association with urate handling. …”
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    GWAS of clinically defined gout and subtypes identifies multiple susceptibility loci that include urate transporter genes
  13. 10393
    “…We will perform lymphocyte opioid receptors genes and proteins expression and functional analysis. Data will be statistically analyzed. …”
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    Lymphocyte opioid receptors as innovative biomarkers of osteoarthritic pain, for the assessment and risk management of opioid tailored therapy, before hip surgery, to prevent chronic pain and opioid tolerance/addiction development: OpMarkArt (Opioids-Markers-Arthroprosthesis) study protocol for a randomized controlled trial
  14. 10394
    “…Subgroup analyses revealed that miR-204-5p could act as a prognostic marker in cancers of respiratory system and digestive system. Functional analysis was conducted on genes predicted as targets (n = 2057) after the overlay genes from six out of twelve software were extracted. …”
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    The protective value of miR-204-5p for prognosis and its potential gene network in various malignancies: a comprehensive exploration based on RNA-seq high-throughput data and bioinformatics
  15. 10395
    “…TILLING (Targeting Induced Local Lesions IN Genomes) is a strategy used for functional analysis of genes that combines the classical mutagenesis and a rapid, high-throughput identification of mutations within a gene of interest. …”
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    HorTILLUS—A Rich and Renewable Source of Induced Mutations for Forward/Reverse Genetics and Pre-breeding Programs in Barley (Hordeum vulgare L.)
  16. 10396
    “…METHODS: EMSA experiments were performed to determine the binding capacity of recombinant TBP and TRF1 to TATA variants, GAAC and GAAC-like boxes. For the functional analysis under different stress stimuli (e.g. growth curve, serum depletion, heat-shock, and UV-irradiation) and during the interaction with mammalian cells (erythrocytes, MDCK cell monolayers, and hepatocytes of hamsters), RT-qPCR, and gene knockdown were performed. …”
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    The Entamoeba histolytica TBP and TRF1 transcription factors are GAAC-box binding proteins, which display differential gene expression under different stress stimuli and during the interaction with mammalian cells
  17. 10397
    “…CONCLUSIONS: The genome-based functional analysis of K. robustum SPU_B003 provided a new understanding of the specific metabolic characteristics. …”
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    Establishing an innovative carbohydrate metabolic pathway for efficient production of 2-keto-l-gulonic acid in Ketogulonicigenium robustum initiated by intronic promoters
  18. 10398
    “…Both sequencing and qPCR tests revealed that PRLR was significantly decreased in the F1 and L2 groups compared to the N1 group, whereas SPEF2 was significantly decreased in the F1 group compared to the N1 or L2 group. Functional analysis revealed that the altered genes or targets of altered miRNAs were involved in multiple biological processes and pathways related to feather growth and development, such as the Wnt signalling pathway, the TGF-beta signalling pathway, the MAPK signalling pathway, epithelial cell differentiation, and limb development. …”
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    Characterization of microRNA and mRNA expression profiles in skin tissue between early-feathering and late-feathering chickens
  19. 10399
    “…To further understand the role of sNKG2DL in pregnancy and individual contributions of the various ligand types, we carried out functional analysis using 181 CBP samples. To test the ability of CBP to suppress the function of NK cells in vitro, we measured expression of NKG2D, CD107a, and IFN-γ in NK cells from control donors after exposure to 181 individual CBP samples and characterised the sMICA, sMICB, and sULBP1 content of each one. …”
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    Functional Characterisation and Analysis of the Soluble NKG2D Ligand Repertoire Detected in Umbilical Cord Blood Plasma
  20. 10400
    “…These findings provide new insights into lncRNAs and lay a foundation for future functional analysis of lncRNAs during early longan SE. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-5158-z) contains supplementary material, which is available to authorized users.…”
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    Genome-wide identification and characterization of long non-coding RNAs involved in the early somatic embryogenesis in Dimocarpus longan Lour
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