“…Loss-of-function analysis is one of the major arsenals we have for understanding gene functions in mammalian cells. …”
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“…Replication-incompetent adenovirus (Ad) vectors have been widely used as gene delivery vehicles in both gene therapy studies and basic studies for gene function analysis due to their highly advantageous properties, which include high transduction efficiencies, relatively large capacities for transgenes, and high titer production. …”
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“…Therefore, the structure function analysis has gained visibility, in particular in combination with imaging techniques such as dynamic shadowgraphy and differential dynamic microscopy. …”
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“…Subsequently, ROS production and mitochondrial function analysis also confirmed that Slc25a43 was a main target gene of oxidative toxicity. …”
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“…Notably, there was no significant difference between healthy children and adults in the throat microbiome, and it was predominated by Firmicutes. In the function analysis, compared with adults, there was increased enrichment in pathways related to amino acid metabolism and lipid metabolism, in children. …”
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“…Four metabolites of G. officinalis were significantly correlated with the abundance of dominant endophyte genera. The predictive function analysis showed that metabolism was main function of endophytic bacteria in different tissue and year root samples, while saprotroph was dominant trophic modes of endophytic fungi in the different year root samples. …”
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“…The aim of the study was to test if it was possible to supplement an existing convolutional neural network (CNN) with post-network calculations for the localization and quantification of posterior glottal gaps during phonation, usable for vocal fold function analysis of e.g. laryngopharyngeal reflux findings. 30 subjects/videos with insufficient closure in the rear glottal area and 20 normal subjects/videos were selected from our database, recorded with a commercial high-speed video setup (HSV with 4000 frames per second), and segmented with an open-source CNN for validating voice function. …”
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“…Meanwhile, according to gene function analysis, SESN2 may be involved in immune responses and the tumor microenvironment (TME). …”
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“…QTAIM and source function analysis were used to explore the non-covalent bonding in twelve different water clusters (H(2)O)(n) obtained by considering n = 2–7 and various geometrical arrangements. …”
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“…Virus-induced gene silencing (VIGS) was used for gene function analysis to determine the CGs’ role in gerbera resistance to Botrytis. …”
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“…Our results showed that G-β-MCA treatment for 5 weeks alleviated ductular reaction and liver fibrosis and improved gut barrier function. Analysis of bile acid metabolism suggested that exogenously administered G-β-MCA was poorly absorbed in the small intestine and mostly deconjugated in the large intestine and converted to taurine-conjugated MCA (T-MCA) in the liver, leading to T-MCA enrichment in the bile and small intestine. …”
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“…The radial distribution function analysis revealed that there were hydrogen bonds between the ethanol and the ADN cations, van der Waals interactions with the ADN anions. …”
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“…When all variables were put into the model, backward stepwise discriminant function analysis isolated lower canine DAR and lower left canine MD as the two independent variables differentiating sex. …”
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“…Our aim was to explore the effects of soybean fermented fowder on lipopolysaccharide (LPS) induced intestinal microflora and corresponding functions in loach. 16S rDNA high-throughout sequencing was applied to estimate differences in the intestinal microbiota and predict genes function. Analysis of the overall of sequencing data showed that the ratio of Effective Tags in both the control group and the treatment group was greater than 80 %. …”
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“…RNA interference (RNAi) by means of short hairpin RNA (shRNA) has developed into a powerful tool for loss-of-function analysis in mammalian cells. The principal problem in RNAi experiments is off-target effects, and the most vigorous demonstration of the specificity of shRNA is the rescue of the RNAi effects with a shRNA-resistant target gene. …”
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“…To test this model, we have performed a detailed structure–function analysis on C-Nap1. We demonstrate that antibody-mediated interference with C-Nap1 function causes centrosome splitting, regardless of the cell cycle phase. …”
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“…Our data suggest that RESC is widely applicable to rescue and validate ubiquitous or tissue-specific RNAi and to perform protein structure–function analysis.…”
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“…We performed a structure-function analysis of cHS4 flanking lentivirus-vectors and analyzed transgene expression in the clonal progeny of hematopoietic stem cells and epigenetic changes in cHS4 and the transgene promoter. …”
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“…Utilizing this model for structure-function analysis, we identified and characterized a gain-of-function mutant of FIT2 (FLL(157-9)AAA) in transmembrane domain 4 that markedly augmented the total number and mean size of lipid droplets. …”
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“…The statistical analysis included the calculation of the Index of Discrimination (D), Discriminant Function Analysis, creation of scales on the basis of the above and then item analysis and calculation of sensitivity and specificity. …”
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