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21por Wookey, Peter, Gupta, Pragya, Bittencourt, Lucas, Cheung, Shane, Hare, David, Furness, Sebastian“…The methods for conjugation and high content imaging on the Operetta platform are described. The high fluorescence intensity at low pH of CalRexin:pHrodo Red in both autophagic and apoptotic cells suggests localisation in autophago-lysosomes and lysosomes respectively. …”
Publicado 2021
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22por Huang, Sisi, Zhang, Sheng, Chen, Limin, Pan, Xiaojun, Wen, Zhenliang, Chen, Yizhu, Zhang, Lidi, Liu, Jiao, Chen, Dechang“…Enzyme-linked immunosorbent assay was used to evaluate the protein levels, hematoxylin and eosin staining was used to evaluate the pathology of the small intestine of mice, and immunohistochemistry and immunofluorescence were used to evaluate the intestinal epithelial barrier function. Perkin Elmer Operetta™ was used to obtain high-resolution images of three-dimensional organoids. …”
Publicado 2022
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23por Yang, Rui, Liu, Na, Chen, Ling, Jiang, Yiqun, Shi, Ying, Mao, Chao, Liu, Yating, Wang, Min, Lai, Weiwei, Tang, Haosheng, Gao, Menghui, Xiao, Desheng, Wang, Xiang, Yu, Fenglei, Cao, Ya, Yan, Qin, Liu, Shuang, Tao, Yongguang“…Cell proliferation assay, cell migration and invasion assays, plate colony formation assay, immunofluorescence assay, Operetta® high-content screening and analysis, Western blot analysis and Co-Immunoprecipitation (Co-IP) assay, RNA immunoprecipitation assay and tumor growth assay was used to address the potential interplay of between GINS4 and LSH, and the functional of GINS4. …”
Publicado 2019
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24“…Calcium level in neurons was measured by Fluo-4-am and Perkin Elmer+ Operetta. Neuronal apoptosis and viability were detected by flow cytometry and the MTS method. …”
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25por Borges, Beatriz Santana, Bueno, Gislayne de Paula, Tomiotto-Pellissier, Fernanda, Figueiredo, Fabiano Borges, Soares Medeiros, Lia Carolina“…For Infection evaluation, fixated cells were stained with Hoechst and read at Operetta High Content Imaging System (Perkin Elmer). …”
Publicado 2023
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26por Gilham, D, Smith, A L, Fu, L, Moore, D Y, Muralidharan, A, Reid, S P M, Stotz, S C, Johansson, J O, Sweeney, M, Wong, N C W, El-Gamal, D, Kulikowski, E“…Infectivity was quantified by determining levels of viral spike protein amongst total cells via imaging on an Operetta CLS. RESULTS: In Calu-3, a human bronchial epithelial cell line, apabetalone dose-dependently downregulated ACE2 gene expression (up to 98%), reduced ACE2 protein levels (up to 84%) and diminished binding of SARS-CoV-2 spike protein (up to 77%, p<0.001 for all parameters). …”
Publicado 2021
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