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4por Massey, Andrew J.“…Here, a high-throughput phenotypic assay utilising the PerkinElmer Operetta high-content imager and Harmony software to determine cell cycle distribution is described. …”
Publicado 2015
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6por Shostakovich, Dmitriĭ Dmitrievich, 1906-1975Tabla de Contenidos: “…Song of challenge : from the film The contest -- Lyrical waltz : from a ballet suite -- Gavotte, from a ballet suite -- Romance, from a ballet suite -- Waltz : a remembrance, from a ballet suite -- Polka, from a ballet suite -- Waltz scherzo, from a ballet suite -- Barrel-organ polka : from a ballet suite -- Waltz of the flowers : from the operetta Moscow, the bird cherries -- Dance, from a ballet suite -- Lullaby, from a ballet suite -- Springtime waltz : from the film Mitchourin -- Country dance : from the film The gadfly -- Spanish dance : from the film The gadfly -- Nocturne, from the film The gadfly -- Barrel-organ waltz : from the film The gadfly -- A little piece : from the film The gadfly -- Sentimental waltz : from a ballet suite -- Skipping dance : from a ballet suite -- Waltz song : from the operetta Moscow, the bird cherries -- Nocturne, from a ballet suite -- Melancholy ditty : from the operetta Moscow, the bird cherries -- Dance, from a ballet suite -- Adagio, from a ballet suite -- Festival waltz : from a ballet suite -- Two duets: Waltz, from the film Unity ; Polka, from a ballet suite.…”
Publicado 1972
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8por Shostakovich, Dmitriĭ Dmitrievich, 1906-1975Tabla de Contenidos: “…Moscow-Cheryomushki : suite from the operetta, op. 105 / edited [i.e. arranged] by Andrew Cornall (19:57) -- The bolt : suite from the ballet, op. 27a : 1934 version (21:17) -- The gadfly : excerpts from the film music, op. 97 : original orchestration (31:29).…”
Publicado 1996
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10“…We used ISD45 or inactivated Vaccinia Virus (VACV) to stimulate cGAS and monitored cellular localization by immunofluorescence microscopy, Operetta high-content screening, and cytoplasmic/nuclear fractionation. …”
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12“…The cells were co-stained with fluorescent dyes (Hoechst 33342 and MITO-ID® Red/MitoPY1/JC-10) and continuously processed by the High Content Imaging System. We employed the Operetta CLS(TM) • We could quantitatively analyze fluorescent images with high-throughput and high-speed detection to track the alteration of mitochondrial status. • The MMP assay is sensitive to FCCP even at the concentration of 0.01 µM. • The fibroblast cells treated with stress inducers (H(2)O(2), FCCP, and phenanthroline) revealed a significant change in mitochondrial health parameters, with more ROS accumulation, depolarized MMP, increased fragmentation, and reduced length of mitochondria.…”
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13por Hart, Christopher J.S., Munro, Taylah, Andrews, Katherine T., Ryan, John H., Riches, Andrew G., Skinner-Adams, Tina S.“…This image-based assay utilizes the Perkin-Elmer Operetta(®) and permits automated assessment of parasite growth at multiple time points without cell-staining. …”
Publicado 2017
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14por Ma, Zhe, Cao, Xuexiao, Guo, Xiao, Wang, Meng, Ren, Xiaoliang, Dong, Ranran, Shao, Rui, Zhu, Yan“…Traditional Chinese medicines (TCMs) have been practiced for over 2000 years in China and East Asia, and herbs are currently used worldwide for the treatment and prevention of chronic and acute disease. Operetta high content analysis (HCA, PerkinElmer, Waltham, MA, USA), which is an in vitro, sensitive, reproducible, multiparametric screening method, was used to evaluate the cytotoxicity of HMPs in cultures of HEK293 human embryo kidney cells. …”
Publicado 2018
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15por Martin, Heather L., Adams, Matthew, Higgins, Julie, Bond, Jacquelyn, Morrison, Ewan E., Bell, Sandra M., Warriner, Stuart, Nelson, Adam, Tomlinson, Darren C.“…This assay has been developed using U2OS cells, the PerkinElmer Operetta high-content/high-throughput imaging system and Columbus image analysis software. …”
Publicado 2014
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16por Souza, Fernanda Gimenez de, Fernandes, Beatriz Luci, Rebelatto, Carmen Lucia Kuniyoshi, Aguiar, Alessandra Melo de, Fracaro, Letícia, Brofman, Paulo Roberto Slud“…Cells were expanded and treated with DMEM-F12 culture, a commercial media for chondrogenic differentiation, and eluate from FRP membrane for three days, and labeled with BrdU for quantitative assessment of cell proliferation using the High-Content Operetta(®) imaging system. For the chondrogenic differentiation assay, the SCATh were grown in micromass for 21 days and stained with toluidine blue and aggrecan for qualitative evaluation by light microscopy. …”
Publicado 2017
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17por Rocha, Vanessa P., Campos, Samir P. C., Barros, Caroline A., Trindade, Pablo, Souza, Leticia R. Q., Silva, Triciana G., Gimba, Etel R. P., Teodoro, Anderson J., Gonçalves, Rafael B.“…For caspases 3/7 activation and reactive oxygen species production evaluation, cells were live-imaged using the high-throughput system Operetta. The cell viability assays demonstrated that bLf induces cell death and morphological changes after 24 h and 48 h of treatment compared to control on DU-145 cells. …”
Publicado 2022
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18por Kowal, Justyna M., Schmal, Hagen, Halekoh, Ulrich, Hjelmborg, Jacob B., Kassem, Moustapha“…We correlated these data with cellular and nuclear morphological features determined by Operetta; a high‐content imaging system. Cell area, cell geometry, and nucleus geometry of cultured hBM‐MSCs exhibited significant correlation with expression of hBM‐MSC membrane markers: ALP, CD146, and CD271. …”
Publicado 2019
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20por Maqoud, Fatima, Curci, Angela, Scala, Rosa, Pannunzio, Alessandra, Campanella, Federica, Coluccia, Mauro, Passantino, Giuseppe, Zizzo, Nicola, Tricarico, Domenico“…The effects of Ca(2+)-activated K(+) (BK) channel modulation by Paxilline (PAX) (10(−7)–10(−4) M), Iberiotoxin (IbTX) (0.1–1 × 10(−6) M) and Resveratrol (RESV) (1–2 × 10(−4) M) on cell cycle and proliferation, AKT1p(Ser473) phosphorylation, cell diameter, and BK currents were investigated in SH-SY5Y cells using Operetta-high-content-Imaging-System, ELISA-assay, impedentiometric counting method and patch-clamp technique, respectively. …”
Publicado 2018
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