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2741por Mangwanda, Ronishree, Zwart, Lizahn, van der Merwe, Nicolaas A., Moleleki, Lucy Novungayo, Berger, Dave Kenneth, Myburg, Alexander A., Naidoo, Sanushka“…In order to understand the molecular interaction between these organisms and predict putative pathogenicity mechanisms of C. austroafricana, fungal gene expression was studied in vitro and in planta. Fungal genes associated with cell wall degradation, carbohydrate metabolism and phytohormone manipulation were expressed in planta by C. austroafricana. …”
Publicado 2016
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2742por Zhang, Weiqi, Li, Jing, Shi, Xiaojun, Hikichi, Yasufumi, Zhang, Yong, Ohnishi, Kouhei“…The aroG1/2 mutants failed to grow in limited medium, but grew slowly in planta. This significantly impaired growth was also observed in the aroG1 mutants both in planta and limited medium, but not in aroG2 mutants. …”
Publicado 2019
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2743
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2744por Köpke, Diana, Schröder, Roland, Fischer, Hanna M., Gershenzon, Jonathan, Hilker, Monika, Schmidt, AxelEnlace del recurso
Publicado 2015
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2745“…This review capitalizes on the advanced technologies used in metabolome mapping in planta, including different metabolomics approaches, imaging, flux analysis, and interpretation using bioinformatics tools. …”
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2746por Demurtas, Olivia Costantina, Sulli, Maria, Ferrante, Paola, Mini, Paola, Martí, Maricarmen, Aragonés, Verónica, Daròs, José-Antonio, Giuliano, Giovanni“…The results also suggested a relaxed substrate specificity of CsCCD2 in planta, cleaving additional carotenoid substrates.…”
Publicado 2023
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2747por Kato, Naohiro, Reynolds, Dexter, Brown, Matthew L, Boisdore, Marietta, Fujikawa, Yukichi, Morales, Andrea, Meisel, Lee A“…BACKGROUND: The isolation of green fluorescent protein (GFP) and the development of spectral variants over the past decade have begun to reveal the dynamic nature of protein trafficking and organelle motility. In planta analyses of this dynamic process have typically been limited to only two organelles or proteins at a time in only a few cell types. …”
Publicado 2008
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2748“…The ccdB protein, however, is not toxic to Agrobacterium tumefaciens, an important player often used for studying gene function in planta. This limits the direct application of the Gateway(® )cloning system in plant transformation-mediated research. …”
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2749“…However, as a means to impart the modifications as early as possible in the starch production chain, similar recombinant enzymes may also be expressed in planta in the developing starch storage organ such as in roots, tubers and cereal grains to provide a GM crop as an alternative to the use of enzymes from GM microorganisms. …”
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2750por Bontinck, Michiel, Van Leene, Jelle, Gadeyne, Astrid, De Rybel, Bert, Eeckhout, Dominique, Nelissen, Hilde, De Jaeger, Geert“…However, studying these specific PPIs in planta can be challenging. One of the most widely adopted methods to study PPIs in planta is affinity purification coupled to mass spectrometry (AP/MS). …”
Publicado 2018
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2751por Ibrahim, Ahmad E. C., van Dolleweerd, Craig J., Drake, Pascal M. W., Ma, Julian K-C.“…However, recovering plant rhabdoviruses from cDNA remains difficult due to technical difficulties such as the need for concurrent in planta expression of the viral genome together with the viral nucleoprotein (N), phosphoprotein (P) and RNA-dependent RNA polymerase (L) and viral genome instability in E. coli. …”
Publicado 2020
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2752por Khakhar, Arjun, Starker, Colby G, Chamness, James C, Lee, Nayoung, Stokke, Sydney, Wang, Cecily, Swanson, Ryan, Rizvi, Furva, Imaizumi, Takato, Voytas, Daniel F“…We also show how our toolbox can be used to deploy the FBP in planta to build auto-luminescent reporters for the study of gene-expression and hormone fluxes. …”
Publicado 2020
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2753por Mao, Xueyun, Hua, Chen, Yang, Liang, Zhang, Yuhui, Sun, Zhengxi, Li, Lei, Li, Tao“…All four of the Se compounds significantly inhibited the mycelial growth and DON production in the in vitro experiment; however, in planta, their effects on FHB severity and toxin accumulation in grains were compound-dependent. …”
Publicado 2020
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2754por Hornyák, Marta, Słomka, Aneta, Sychta, Klaudia, Dziurka, Michał, Kopeć, Przemysław, Pastuszak, Jakub, Szczerba, Anna, Płażek, Agnieszka“…To verify the hypothesis, we studied nutrient stress in vitro and in planta and analyzed different embryological and yield parameters, including hormone profile in the flowers. …”
Publicado 2020
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2755por Heyman, Lisa, Chrysargyris, Antonios, Demeestere, Kristof, Tzortzakis, Nikolaos, Höfte, Monica“…Drought stress increased the susceptibility of grapevine leaves inoculated in vitro, but both cultivars showed resistance against P. viticola when inoculated in planta. Abscisic acid, rather than jasmonic acid and salicylic acid, seemed to play a prominent role in this resistance. …”
Publicado 2021
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2756por Cellini, Antonio, Donati, Irene, Farneti, Brian, Khomenko, Iuliia, Buriani, Giampaolo, Biasioli, Franco, Cristescu, Simona M., Spinelli, Francesco“…Ethylene production is modulated in planta by light/dark cycle. Exogenous ethylene application stimulates bacterial virulence, and restricts or increases host colonisation if performed before or after inoculation, respectively. …”
Publicado 2021
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2757“…In this study, we describe an additional feature of an in planta gene-stacking scheme, in which the Cre (control of recombination) recombinase not only deletes transgenic DNA no longer needed after transformation but also mediates recombination between homologous or non-homologous chromosomes. …”
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2758“…Despite their contrasting roles in planta, the salicylic acid and jasmonic acid defence networks share a common architecture, progressing from stages of biosynthesis, to modification, regulation, and response. …”
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2759“…With growing interest in isoflavonoids from both research and industrial perspectives, efforts are being forwarded to enhance isoflavonoid production in-planta and ex-planta; therefore, in-silico analysis and characterisation of available IFS protein sequences are needed. …”
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2760