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3261“…Differential expression patterns of several genes were validated by quantitative real-time PCR (qRT-PCR) and northern blotting. In planta qRT-PCR of several selected genes also showed differential expression patterns during infection and disease progression. …”
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3262por Yoneyama, Kaori, Xie, Xiaonan, Kim, Hyun Il, Kisugi, Takaya, Nomura, Takahito, Sekimoto, Hitoshi, Yokota, Takao, Yoneyama, KoichiEnlace del recurso
Publicado 2011
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3263por Gonzales-Vigil, Eliana, Hufnagel, David E, Kim, Jeongwoon, Last, Robert L, Barry, Cornelius S“…These produce either the monoterpenes α-pinene and limonene, or the sesquiterpene 7-epizingiberene, with the in vitro analyses that recapitulated the trichome chemistry found in planta. Comparison of the distribution of S. habrochaites accessions with terpene content revealed a strong preference for the presence of particular TPS20 alleles at distinct geographic locations. …”
Publicado 2012
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3264“…Previously, we reported that artificial zinc-finger proteins (AZPs) inhibited virus DNA replication in planta and in mammalian cells by blocking binding of a viral replication protein to its replication origin. …”
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3266por Ramirez, Aldana M., Saillard, Nils, Yang, Ting, Franssen, Maurice C. R., Bouwmeester, Harro J., Jongsma, Maarten A.“…Candidate genes implicated in three committed steps leading from farnesyl diphosphate to STL and other oxygenated derivatives of germacratrien-12-oic acid were retrieved from a pyrethrum trichome EST library, cloned, and characterized in yeast and in planta. The diversity and distribution of sesquiterpene lactones in different tissues and the correlation with the expression of these genes are shown and discussed.…”
Publicado 2013
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3267por Guy, Endrick, Lautier, Martine, Chabannes, Matthieu, Roux, Brice, Lauber, Emmanuelle, Arlat, Matthieu, Noël, Laurent D.“…Furthermore, xopAC can also confer avirulence to both the vascular pathogen Ralstonia solanacearum and the mesophyll-colonizing pathogen Pseudomonas syringae indicating that xopAC-specified effector-triggered immunity is not specific to the vascular system. In planta, XopAC-YFP fusions are localized at the plasma membrane suggesting that XopAC might interact with membrane-localized proteins. …”
Publicado 2013
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3268por Graumann, Katja“…This recruitment and the immobile properties are likely due to AtSUN1/2-AtLINC1 protein interactions occurring in planta. In addition, the SUN N-terminus appears to play an important role in mediating these interactions. …”
Publicado 2014
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3269por Wang, Caixia, Li, Chao, Li, Baohua, Li, Guifang, Dong, Xiangli, Wang, Guoping, Zhang, Qingming“…However, this correlation only existed in planta, but not in vitro. During the infection of Vmm, protocatechuic acid was first detected at three days post inoculation (dpi), and the others at seven or 11 dpi. …”
Publicado 2014
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3270Expression of a Translationally Fused TAP-Tagged Plasma Membrane Proton Pump in Arabidopsis thalianapor Rodrigues, Rachel B., Sabat, Gregorz, Minkoff, Benjamin B., Burch, Heather L., Nguyen, Thao T., Sussman, Michael R.“…In conclusion, we have generated transgenic Arabidopsis lines in which a TAP-tagged AHA1 transgene has complemented all essential endogenous AHA1 and AHA2 functions and have shown that these plants can be used to purify AHA1 protein and to identify in planta interacting proteins by mass spectrometry.…”
Publicado 2014
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3271“…Here, we report that a peptide LMX, optimized from the insect-specific scorpion neurotoxin LqhIT2, showed high levels of activity against rice leaf folder in vitro and in planta. Oral ingestion of LMX protein led to a significant decrease in feeding on rice leaves, repression of larval growth and development, delay in molting, and increase in larval lethality. …”
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3272“…RESULTS: Here, we report in planta imaging of single RNA molecules using fluorescently labeled molecular beacons. …”
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3273Photosynthesis in a different light: spectro-microscopy for in vivo characterization of chloroplastspor Peter, Sébastien, Zell, Martina B., Blum, Christian, Stuhl, Alexander, Elgass, Kirstin, Sackrow, Marcus, Subramaniam, Vinod, Meixner, Alfred J., Harter, Klaus, Maurino, Veronica G., Schleifenbaum, Frank E.“…Here, we use non-invasive in planta spectro-microscopic approaches to investigate living chloroplasts in their native environment at ambient temperatures. …”
Publicado 2014
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3274“…We propose that numerous Salmonella or E. coli effectors may be active in planta and tend to target central components (hubs) of immune signaling pathways.…”
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3275por Hentrich, Mathias, Sánchez-Parra, Beatriz, Pérez Alonso, Marta-Marina, Carrasco Loba, Víctor, Carrillo, Laura, Vicente-Carbajosa, Jesus, Medina, Joaquín, Pollmann, Stephan“…Both gene products were demonstrated to contribute to auxin biosynthesis in planta.(1) Here we report that the overexpression of YUC8 as well as YUC9 led to strong lignification of plant aerial tissues. …”
Publicado 2013
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3276
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3277“…To identify genes responsible for natural variation in primary metabolism, we focused on cloning a locus from Arabidopsis thaliana that influences the level of TCA cycle metabolites in planta. We found that the Met.V.67 locus was controlled by natural variation in METABOLIC NETWORK MODULATOR 1 (MNM1), which encoded an AT-hook motif-containing protein that was unique to the Brassicales lineage. …”
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3278por Nam, Jiryun, Nam, Moon, Bae, Hanhong, Lee, Cheolho, Lee, Bong-Chun, Hammond, John, Lim, Hyoun-Sub“…The yeast two hybrid system and Bimolecular Fluorescence Complementation (BiFC) in planta were utilized to examine homologous interactions of the mutants. …”
Publicado 2013
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3279por Eves-van den Akker, Sebastian, Lilley, Catherine J., Jones, John T., Urwin, Peter E.“…We have identified HYP effectors in three genera of plant parasitic nematodes capable of infecting a broad range of mono- and dicotyledon crop species. In planta RNAi targeted to all members of the effector family causes a reduction in successful parasitism.…”
Publicado 2014
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3280por Sieber, Christian M. K., Lee, Wanseon, Wong, Philip, Münsterkötter, Martin, Mewes, Hans-Werner, Schmeitzl, Clemens, Varga, Elisabeth, Berthiller, Franz, Adam, Gerhard, Güldener, Ulrich“…Based on our analysis we present 67 gene clusters with significant enrichment of predicted secondary metabolism related enzymatic functions. 20 gene clusters with unknown metabolites exhibit strong gene expression correlation in planta and presumably play a role in virulence. Furthermore, the identification of conserved and over-represented putative transcription factor binding sites serves as additional evidence for cluster co-regulation. …”
Publicado 2014
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