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3461“…Inhibition of mitochondrial Sdh enzyme activity and control efficacy in planta for those isolates was severely impaired by fluopyram and isofetamid, but not by bixafen. …”
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3462“…Studies from plants exposed to chemical inhibitors of animal CCC protein function are reviewed for their potential to discern CCC function in planta. Thus far, mutations in plant CCC genes have been evaluated only in two species of angiosperms, and such mutations cause a diverse array of phenotypes—seemingly more than could simply be explained by localized disruption of ion transport alone. …”
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3463por Flobinus, Alyssa, Chevigny, Nicolas, Charley, Phillida A., Seissler, Tanja, Klein, Elodie, Bleykasten-Grosshans, Claudine, Ratti, Claudio, Bouzoubaa, Salah, Wilusz, Jeffrey, Gilmer, David“…Substitution of the BNYVV-RNA3 ‘core’ sequence by the sfRNA sequence led to the accumulation of an ncRNA species in yeast in vitro but not in planta and no viral long distance occurred. Interestingly, XRN4 knockdown reduced BNYVV RNA accumulation suggesting a dual role for the ribonuclease in the viral cycle.…”
Publicado 2018
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3464por Dalakouras, Athanasios, Jarausch, Wolfgang, Buchholz, Guenther, Bassler, Alexandra, Braun, Mario, Manthey, Thorsten, Krczal, Gabi, Wassenegger, Michael“…Recently, transgene-free procedures that were based on exogenous application of RNA molecules having the capacity to trigger RNAi in planta have been reported. Yet, efficient delivery of such RNA molecules to plants and particularly to trees poses major technical challenges. …”
Publicado 2018
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3465por Torre, Covadonga, Donaire, Livia, Gómez-Aix, Cristina, Juárez, Miguel, Peterschmitt, Michel, Urbino, Cica, Hernando, Yolanda, Agüero, Jesús, Aranda, Miguel A.“…Thus, an infectious Group 2 clone (TYLCV-Mu15) was constructed and used in in planta competition assays against TYLCV-IS76. TYLCV-Mu15 predominated in single infections, whereas TYLCV-IS76 did so in mixed infections, providing credibility to a scenario of co-occurrence of both types of isolates.…”
Publicado 2018
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3466“…The aim of this study was to enhance carbohydrate accumulation in transgenic Nicotiana benthamiana by simultaneously expressing the maize Corngrass1 miRNA (Cg1) and E. coli ADP-glucose pyrophosphorylase (glgC), both of which have been reported to enhance carbohydrate accumulation in planta. Our findings revealed that expression of Cg1 alone increased shoot branching, delayed flowering, reduced flower organ size, and induced loss of fertility. …”
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3467por Topman, Shiri, Tamir‐Ariel, Dafna, Bochnic‐Tamir, Heli, Stern Bauer, Tal, Shafir, Sharoni, Burdman, Saul, Hayouka, Zvi“…Both RPMs displayed powerful bacteriostatic and bactericidal activities towards strains belonging to several plant pathogenic bacterial genera, for example, Xanthomonas, Clavibacter and Pseudomonas. In planta studies in the glasshouse revealed that RPMs significantly reduced disease severity of tomato and kohlrabi plants infected with Xanthomonas perforans and Xanthomonas campestris pv. campestris respectively. …”
Publicado 2018
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3468por Aviña-Padilla, Katia, Rivera-Bustamante, Rafael, Kovalskaya, Natalia Y., Hammond, Rosemarie W.“…Dramatic alterations in plant development triggered by viroid infection are the result of differential gene expression; in our study, we focused on the effect of tomato planta macho viroid (TPMVd) and Mexican papita viroid (MPVd) infection on gene networks associated with the regulation of flower and fruit development. …”
Publicado 2018
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3469por Zhang, Jian-Ling, Wang, Dan, Liang, Yu-Wei, Zhong, Wan-Ying, Ming, Zhen-Hua, Tang, Dong-Jie, Tang, Ji-Liang“…Disruption of the operon resulted in defective growth in methionine-limited media and in planta. Western blot analysis revealed that the expression of the operon is dependent on methionine levels. …”
Publicado 2018
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3470por Lekeux, Gilles, Laurent, Clémentine, Joris, Marine, Jadoul, Alice, Jiang, Dan, Bosman, Bernard, Carnol, Monique, Motte, Patrick, Xiao, Zhiguang, Galleni, Moreno, Hanikenne, Marc“…We show that di-Cys motifs, but not the His-stretch, contribute to high affinity zinc binding and function in planta. We suggest that the HMA4 C-terminal extension is at least partly responsible for protein targeting to the plasma membrane. …”
Publicado 2018
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3471por Neve, Paul“…Technical challenges relating to plant transformation and gene editing in planta are considered, and the implementation of gene drives for timely and sustainable weed management is reviewed in the light of weed population biology. …”
Publicado 2018
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3472por Zhang, Jie, Zhu, Qiusha, Yu, Haijuan, Li, Liang, Zhang, Guoqiang, Chen, Xi, Jiang, Mingyi, Tan, Mingpu“…Moreover, they were confirmed to be Cd-tolerance genes in planta through lesion analysis in Cd-infiltrated leaves transiently expressing them. …”
Publicado 2019
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3473“…A number of mutants that are useful to study ERQC in planta have been described in the literature, but only for a subset of these mutants the innate immune response to bacterial elicitors has been measured beyond monitoring plant weight and some physio-pathological parameters related to the plant immune response. …”
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3474“…Type of amino acids at position 69 in Avrblb2, which determines Rbi-blb2 resistance protein-mediated defense responses, is not involved in the Avrblb2-calmodulin interaction. Using in planta functional analyses, we show that calmodulin binding to Avrblb2 is required for its recognition by Rpi-blb2 to incite hypersensitive response. …”
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3475por Zeng, Jianyan, Zhang, Mi, Hou, Lei, Bai, Wenqin, Yan, Xingying, Hou, Nan, Wang, Hongxing, Huang, Juan, Zhao, Juan, Pei, Yan“…In this study, we demonstrate an antagonistic effect between cytokinin (CK) and auxin on cotton fiber initiation. In vitro and in planta experiments indicate that enhanced CK levels can reduce auxin accumulation in the ovule integument, which may account for the defects in the fiberless mutant xu142fl. …”
Publicado 2019
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3476por Kežar, Andreja, Kavčič, Luka, Polák, Martin, Nováček, Jiří, Gutiérrez-Aguirre, Ion, Žnidarič, Magda Tušek, Coll, Anna, Stare, Katja, Gruden, Kristina, Ravnikar, Maja, Pahovnik, David, Žagar, Ema, Merzel, Franci, Anderluh, Gregor, Podobnik, Marjetka“…Together with mutational analysis and in planta experiments, we show their crucial role in PVY infectivity and explain the ability of the coat protein to perform multiple biological tasks. …”
Publicado 2019
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3477“…Following our screening, in planta competition assays confirmed that the uridine monophosphate biosynthesis pathway and the purine biosynthesis pathway were essential to the survival of D. dadantii in the plant, as the mutants ∆carA, ∆purF, ∆purL, ∆guaB and ∆pyrE were unable to survive in the plant in contrast with the wild‐type (WT) bacterium. …”
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3478“…Furthermore, the expression of RipN in Arabidopsis suppresses callose deposition and the transcription of pathogen‐associated molecular pattern (PAMP)‐triggered immunity (PTI) marker genes under flg22 treatment, and promotes bacterial growth in planta. In addition, the expression of RipN in plant cells alters NADH/NAD(+), but not GSH/GSSG, ratios, and its Nudix hydrolase activity is indispensable for such biochemical function. …”
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3479por Fischer, Jochen, Beckers, Sebastian J., Yiamsawas, Doungporn, Thines, Eckhard, Landfester, Katharina, Wurm, Frederik R.“…The specific antifungal activity is confirmed in vitro and in planta (in Vitis vinifera L. cv. ‘Portugieser’). All treated plants prove to exhibit significantly fewer symptoms several weeks after treatment, and their condition is monitored for 5 years (2014–2018), proving a long‐term curative effect of this NC treatment. …”
Publicado 2019
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3480por Liu, Siwen, Wu, Bo, Yang, Jing, Bi, Fangcheng, Dong, Tao, Yang, Qiaosong, Hu, Chunhua, Xiang, Dandan, Chen, Hongrui, Huang, Huoqing, Shao, Chuange, Chen, Yixiang, Yi, Ganjun, Li, Chunyu, Guo, Xiuwu“…The FocCP1 gene was expressed in every condition studied, showing a high transcript level in planta at the early stage of infection. Infiltration of the recombinant FocCP1 protein induced significant cell death and upregulated defence-related gene expression. …”
Publicado 2019
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