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3661por Eudes, Aymerick, Dutta, Tanmoy, Deng, Kai, Jacquet, Nicolas, Sinha, Anagh, Benites, Veronica T., Baidoo, Edward E. K., Richel, Aurore, Sattler, Scott E., Northen, Trent R., Singh, Seema, Simmons, Blake A., Loqué, Dominique“…It has recently been demonstrated in rice and maize that caffeate O-methyltransferase (COMT) involved in the synthesis syringyl (S) lignin units derived from sinapyl alcohol also participates in the synthesis of tricin in planta. In this work, we validate in sorghum (Sorghum bicolor L.) that the O-methyltransferase responsible for the production of S lignin units (SbCOMT / Bmr12) is also involved in the synthesis of lignin-linked tricin. …”
Publicado 2017
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3662“…METHODS: Gene expression patterns of PgCPT1 were analyzed by quantitative reverse transcription polymerase chain reaction. In planta transformation was generated by floral dipping using Agrobacterium tumefaciens. …”
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3663por Kahla, Yosra, Zouari-Bouassida, Karama, Rezgui, Fatma, Trigui, Mohamed, Tounsi, Slim“…The time-kill curve assay of EtOAc-F (2.5 mg/mL) showed a potent bactericidal effect after 20 min of direct contact with A. tumefaciens. In planta experiments, gall weights were significantly reduced when EtOAc-F was applied at 0.625 and 2.5 mg/wounds. …”
Publicado 2017
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3664por Luo, Yuan, Chen, Qingguo, Luan, Junbo, Chung, Seung Ho, Van Eck, Joyce, Turgeon, R., Douglas, Angela E.“…In planta RNAi against essential insect genes offers a promising route to control insect crop pests, but is constrained for many insect groups, notably phloem sap-feeding hemipterans, by poor RNAi efficacy. …”
Publicado 2017
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3665por Wang, Shumei, Boevink, Petra C., Welsh, Lydia, Zhang, Ruofang, Whisson, Stephen C., Birch, Paul R. J.“…We used transient Agrobacterium tumefaciens‐mediated in planta expression, transformation of P. infestans with fluorescent protein fusions and confocal microscopy to investigate delivery of effectors to plant cells during infection. …”
Publicado 2017
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3666por Zhang, Yong, Li, Jing, Zhang, Weiqi, Wang, Rongsheng, Qiu, Qiaoqing, Luo, Feng, Hikichi, Yasufumi, Ohnishi, Kouhei, Ding, Wei“…However, exogenously supplemented FA did not affect the T3SS expression in planta and the elicitation of the hypersensitive response (HR) in tobacco leaves. …”
Publicado 2017
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3667por Nakel, Thomas, Tekleyohans, Dawit G., Mao, Yanbo, Fuchert, Golo, Vo, Dieu, Groß-Hardt, Rita“…We established a high-throughput polyspermy detection assay, which uncovered that supernumerary sperm fusion does occur in planta and can generate viable polyploid offspring. …”
Publicado 2017
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3668“…Brome mosaic virus (BMV)-induced RNA interference (RNAi) has emerged as a useful tool to study host-resistance genes for rice blast protection. Planta-generated silencing of targeted genes inside biotrophic pathogens can be achieved by expression of M. oryzae-derived gene fragments in the BMV-mediated gene silencing system, a technique termed host-induced gene silencing (HIGS). …”
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3669“…To assess the importance of the individual chromosomes during host infection, we performed in planta assays comparing disease development results in wild-type and chromosome mutant strains. …”
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3670por Oehlenschlæger, Christian B., Gersby, Lotte B. A., Ahsan, Nagib, Pedersen, Jesper T., Kristensen, Astrid, Solakova, Tsvetelina V., Thelen, Jay J., Fuglsang, Anja T.“…We show that full-length PSY1R interacts with members of the SERK co-receptor family in planta. We identified seven in vitro autophosphorylation sites on serine and threonine residues within the kinase domain of PSY1R using mass spectrometry. …”
Publicado 2017
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3671por Saravana Kumar, R. M., Gao, Liu Xiao, Yuan, Hu Wei, Xu, Dong Bin, Liang, Zhao, Tao, Shen Chen, Guo, Wen Bin, Yan, Dao Liang, Zheng, Bing Song, Edqvist, JohanEnlace del recurso
Publicado 2017
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3672por Hill, Benjamin L, Figueroa, Carlos M, Asencion Diez, Matías D, Lunn, John E, Iglesias, Alberto A, Ballicora, Miguel A“…Moreover, their calculated in vitro half-lives are significantly higher than estimates of their in planta turnover times. This indicates that the physico-chemical stability of NDP-sugars has little impact on their concentrations in vivo and that NDP-sugar levels are determined primarily by the relative rates of enzymatic synthesis and consumption. …”
Publicado 2017
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3673
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3674por Brewer, Helen C., Hird, Diane L., Bailey, Andy M., Seal, Susan E., Foster, Gary D.“…Plant viruses are also increasingly being investigated as expression vectors for in planta production of pharmaceutical products, known as molecular farming. …”
Publicado 2018
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3675por Machado, Ana Karla, Brown, Neil A, Urban, Martin, Kanyuka, Kostya, Hammond‐Kosack, Kim E“…Host‐induced gene silencing (HIGS) is a transgenic technology used to silence fungal genes in planta during attempted infection and thereby reduces disease levels. …”
Publicado 2017
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3676por Iseki, Moe, Shida, Kasumi, Kuwabara, Kazuma, Wakabayashi, Takatoshi, Mizutani, Masaharu, Takikawa, Hirosato, Sugimoto, Yukihiro“…Dissecting common and divergent biosynthetic pathways of SLs may provide avenues for modulating their production in planta. Biosynthesis of SLs in various SL-producing plant species was inhibited by fluridone, a phytoene desaturase inhibitor. …”
Publicado 2018
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3677“…Despite recent advances towards the elucidation of CRN virulence functions, the relevance of CRN‐induced cell death remains unclear. In planta over‐expression of PcCRN83_152, a CRN effector from Phytophthora capsici, causes host cell death and boosts P. capsici virulence. …”
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3678por Minina, Elena A, Moschou, Panagiotis N, Vetukuri, Ramesh R, Sanchez-Vera, Victoria, Cardoso, Catarina, Liu, Qinsong, Elander, Pernilla H, Dalman, Kerstin, Beganovic, Mirela, Lindberg Yilmaz, Jenny, Marmon, Sofia, Shabala, Lana, Suarez, Maria F, Ljung, Karin, Novák, Ondřej, Shabala, Sergey, Stymne, Sten, Hofius, Daniel, Bozhkov, Peter V“…This work provides an experimental paradigm and mechanistic insight into genetic stimulation of autophagy in planta and shows its efficiency for improving plant productivity.…”
Publicado 2018
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3679por Nottensteiner, Mathias, Zechmann, Bernd, McCollum, Christopher, Hückelhoven, Ralph“…Bimolecular fluorescence complementation and co-localization supported that ROPIP1 can interact with activated HvRACB in planta. We show that ROPIP1 is expressed by Bgh on barley and translocated into the cytoplasm of infected barley cells. …”
Publicado 2018
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3680