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3881“…We found a significant decrease in cell-cell aggregation among PD1063 mutants but no differences in cell growth, biofilm formation, disease severity or titers in planta. Based on the demonstration that Xanthomonas oryzae pv. oryzae PXO_03968 encodes an outer membrane protein, secreted in association with outer membrane vesicles, we predicted that PD1063 would also be secreted in a similar manner. …”
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3882por Ha, Chien Van, Nasr Esfahani, Maryam, Watanabe, Yasuko, Tran, Uyen Thi, Sulieman, Saad, Mochida, Keiichi, Van Nguyen, Dong, Tran, Lam-Son Phan“…Our results have provided a solid foundation for selection of promising tissue-specific and/or dehydration-responsive CaNAC candidates for detailed in planta functional analyses, leading to development of transgenic chickpea varieties with improved productivity under drought.…”
Publicado 2014
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3883por Palmer, Richard, Cornuault, Valérie, Marcus, Susan E., Knox, J. Paul, Shewry, Peter R., Tosi, PaolaEnlace del recurso
Publicado 2014
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3884por Acheampong, Atiako Kwame, Hu, Jianhong, Rotman, Ariel, Zheng, Chuanlin, Halaly, Tamar, Takebayashi, Yumiko, Jikumaru, Yusuke, Kamiya, Yuji, Lichter, Amnon, Sun, Tai-Ping, Or, Etti“…Additionally, expression of these grape genes in corresponding Arabidopsis mutants confirmed their functions in planta. Spatiotemporal analysis of VvDELLAs showed that both VvDELLA1 and VvDELLA2 are abundant in most tissues, except in developing fruit where VvDELLA2 is uniquely expressed at high levels, suggesting a key role in fruit development. …”
Publicado 2015
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3885
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3886“…The Ser49 residue of C4 was found to be critical for C4 function, since: 1) mutagenesis of Ser49 to Ala abolished the C4-induced phenotype, abolished C4/AtSK interactions, and resulted in a mutant protein that failed to induce changes in the BR signaling pathway; 2) Ser49 is phosphorylated in planta; and 3) plant-encoded AtSKs must be catalytically active to interact with C4. …”
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3887por Kumar, Deepak, Datta, Riddhi, Hazra, Saptarshi, Sultana, Asma, Mukhopadhyay, Ria, Chattopadhyay, Sharmila“…In this study transcriptome analysis of pad2.1, an Arabidopsis thaliana mutant, after combined osmotic and cold stress treatment has been performed to explore the intricate position of GSH in the stress and defense signaling network in planta. Microarray data revealed the differential regulation of about 1674 genes in pad2.1 amongst which 973 and 701 were significantly up- and down-regulated respectively. …”
Publicado 2015
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3888
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3889por Koch, Michael S., Ward, Jason M., Levine, Steven L., Baum, James A., Vicini, John L., Hammond, Bruce G.“…The Cry gene sequences are often modified to enable effective expression in planta and several Cry proteins have been modified to increase biological activity against the target pest(s). …”
Publicado 2015
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3890por Gao, Fei, Wang, Jianyue, Wei, Shanjun, Li, Zhanglei, Wang, Ning, Li, Huayun, Feng, Jinchao, Li, Hongjie, Zhou, Yijun, Zhang, Feixiong“…The differentially expressed transcripts identified in our study provide a good start for identifying the key genes in stress response and performing functional analysis to reveal their roles in stress adaptation in planta.…”
Publicado 2015
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3891por Ding, Fang, Duan, Yongping, Paul, Cristina, Brlansky, Ronald H., Hartung, John S.“…Our data provide direct spatial and anatomical information for CaLas in planta. This simple and scalable method may facilitate the future research on the interaction of CaLas and host plant.…”
Publicado 2015
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3892por Cregeen, Sara, Radisek, Sebastjan, Mandelc, Stanislav, Turk, Boris, Stajner, Natasa, Jakse, Jernej, Javornik, Branka“…In the resistant cultivar, the RT-qPCR expression patterns of most genes showed their peak at 20 dpi and declined towards 30 dpi, comparable to the gene expression pattern of in planta detected fungal protein and coinciding with the highest fungal biomass in plants at 15 dpi. …”
Publicado 2014
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3893“…Bimolecular fluorescence complementation and co-immunoprecipitation assays confirmed the interaction between CaALDH1 and AvrBsT in planta. CaALDH1:smGFP fluorescence was detected in the cytoplasm. …”
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3894por Zhou, Jinggeng, Lu, Dongping, Xu, Guangyuan, Finlayson, Scott A., He, Ping, Shan, Libo“…Overexpression of the PUB13 ARM domain alone inhibits flg22-induced FLS2–PUB13 association and PUB12/13-mediated FLS2 ubiquitination and degradation in Arabidopsis, suggesting that ectopic expression of the ARM domain in planta generates a dominant negative effect via blocking the ubiquitination activity. …”
Publicado 2015
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3895por Tang, Chunlei, Wei, Jinping, Han, Qingmei, Liu, Rui, Duan, Xiaoyuan, Fu, Yanping, Huang, Xueling, Wang, Xiaojie, Kang, Zhensheng“…Further study showed that the three mito-carr domains are all needed to induce cell death. qRT-PCR analyses revealed an in-planta induced expression of PsANT during infection. …”
Publicado 2015
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3896“…This, along with the HPLC characterization of high residual and non-utilized quercetin in presence of the knockout mutants, indicates the involvement of VdQase in the catabolism of quercetin and possibly other flavonols in planta. Quantification of Salicylic and Jasmonic Acids (SA, JA) in response to the mutants vs. wild type isolates revealed involvement of VdQase in the interference with signaling, suggesting a role in pathogenicity. …”
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3897“…In the present study, we developed a faster and more efficient in vitro recombination system using Gibson assembly (GA), to engineer a Lettuce mosaic virus (LMV) infectious clone expressing an ectopic mcherry-tagged VPg (Viral protein genome-linked) for in planta subcellular localization of the viral protein in an infection context. …”
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3898por Withanage, Samanthi Priyanka, Hossain, Md Aktar, Kumar M., Sures, Roslan, Hairul Azman B, Abdullah, Mohammad Puad, Napis, Suhaimi B., Shukor, Nor Aini Ab.“…AtGA20ox gene with intron was overexpressed in kenaf plants under the control of double CaMV 35S promoter, followed by in planta transformation into V36 and G4 varieties of kenaf. …”
Publicado 2015
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3899por Chou, Chien-Ming, Yu, Fang-Yi, Yu, Pei-Ling, Ho, Jia-Fang, Bostock, Richard M., Chung, Kuang-Ren, Huang, Jenn-Wen, Lee, Miin-Huey“…MfPG1 encodes the major endo-PG and is expressed to significantly higher levels compared to the other four MfPGs in culture and in planta. MfPG1 function during pathogenesis was evaluated by examining the disease phenotypes and gene expression patterns in M. fructicola MfPG1-overexpressing strains and in strains carrying the β-glucuronidase (GUS) reporter gene fused with MfPG1 (MfPG1-GUS). …”
Publicado 2015
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3900por Mir, Albely Afifa, Park, Sook-Young, Sadat, Md. Abu, Kim, Seongbeom, Choi, Jaeyoung, Jeon, Junhyun, Lee, Yong-Hwan“…Expression profiles showed that majority of them are responsive to in planta condition and in vitro H(2)O(2). Our analysis of individual deletion mutants for seven selected genes including MoPRX1 revealed that these genes contribute to fungal development and/or pathogenesis. …”
Publicado 2015
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