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4143por Fu, Jianmei, Shi, Yu, Wang, Lihua, Tian, Tian, Li, Jing, Gong, Lei, Zheng, Zhouting, Jing, Maofeng, Fang, Jichao, Ji, Rui“…Bacterially expressed recombinant CaM protein exhibited calcium-binding activity. In planta expression disclosed that CaM was localized to the plant cytoplasms and nuclei and suppressed plant defenses such as hydrogen peroxide (H(2)O(2)) accumulation and callose deposition. …”
Publicado 2022
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4144por Selles, Benjamin, Moseler, Anna, Caubrière, Damien, Sun, Sheng-Kai, Ziesel, Morgane, Dhalleine, Tiphaine, Hériché, Mathilde, Wirtz, Markus, Rouhier, Nicolas, Couturier, Jérémy“…We also show that both proteins interact in planta and form an efficient sulfur relay system, whereby STR18 catalyzes transpersulfidation reactions from ABA3 to the model acceptor protein roGFP2. …”
Publicado 2022
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4145“…Finally, the importance to explore the subcellular localization of UGTs to study their roles in planta is discussed.…”
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4146por Kulić, Žarko, Ritter, Thomas, Röck, Birgit, Elsäßer, Jens, Schneider, Heike, Germer, StefanEnlace del recurso
Publicado 2021
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4147por Arellano-Saab, Amir, McErlean, Christopher S.P., Lumba, Shelley, Savchenko, Alexei, Stogios, Peter J., McCourt, Peter“…Furthermore, DOZ reduces the flexibility of protein–protein interaction domains important for receptor signaling to downstream partners. In planta, we show, via temporal additions of DOZ, that SL receptors are required at a specific time during seed conditioning. …”
Publicado 2022
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4148“…To conduct these analyses, F. defendens was cultured in a range of media types, while associated inhibitory effects were tested in vitro using Spiroplasma melliferum as a model mollicute bacterium, and in planta using phytoplasma-infected periwinkle plantlets. …”
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4149por Smith, Rebecca A, Beebe, Emily T, Bingman, Craig A, Vander Meulen, Kirk, Eugene, Alexis, Steiner, Alexander J, Karlen, Steven D, Ralph, John, Fox, Brian G“…These putative FMTs and PMTs were transformed into Arabidopsis (Arabidopsis thaliana) to test their activities and abilities to biosynthesize monolignol conjugates for lignification in planta. The presence of ferulates and p-coumarates on the lignin of these transformants indicated that the putative FMTs and PMTs act as functional feruloyl-CoA and p-coumaroyl-CoA monolignol transferases within plants.…”
Publicado 2022
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4150“…A comprehensive study on the pollen ontogeny of diploid and doubled diploid “Sanguinelli” blood orange and “Clemenules” clementine was performed, with focus on pollen grain germination in vitro and in planta, morphology of mature pollen grains by scanning electron microscopy (SEM), cytochemical characterization of carbohydrates by periodic acid–Shiff staining, and specific cell wall components revealed by immunolocalization. …”
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4151por Meshram, Shweta, Gogoi, Robin, Bashyal, Bishnu Maya, Kumar, Aundy, Mandal, Pranab Kumar, Hossain, Firoz“…In the present study, we conducted an in planta transcriptomics study of the B. maydis race “O” pathogen after infection on non-CMS maize resistant and susceptible genotypes by mRNA sequencing to understand the molecular basis of pathogenicity for better management of the pathogen. …”
Publicado 2022
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4152por Zhao, Yulei, Agrawal, Nisha, Ghareeb, Hassan, Habib, Mohammad Tanbir, Dickmeis, Sascha, Schwachtje, Jens, Iven, Tim E., Kopka, Joachim, Feussner, Ivo, Schirawski, Jan“…Interaction of the two proteins in planta was confirmed by bimolecular fluorescence complementation. qRT-PCR experiments revealed vag2-dependent downregulation of salicylic acid (SA)-induced genes, which correlated with higher SA levels in plant tissues colonized by Δvag2 deletion strains relative to S. reilianum wildtype strains. …”
Publicado 2022
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4153por Hemara, Lauren M., Jayaraman, Jay, Sutherland, Paul W., Montefiori, Mirco, Arshed, Saadiah, Chatterjee, Abhishek, Chen, Ronan, Andersen, Mark T., Mesarich, Carl H., van der Linden, Otto, Yoon, Minsoo, Schipper, Magan M., Vanneste, Joel L., Brendolise, Cyril, Templeton, Matthew D.“…Screening of a complete library of Psa3 effector knockout strains identified increased growth in planta for knockouts of four effectors–AvrRpm1a, HopF1c, HopZ5a, and the EEL effector HopAW1a –suggesting a resistance response in A. arguta. …”
Publicado 2022
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4154por Sadre, Radin, Anthony, Thilani M., Grabar, Josh M., Bedewitz, Matthew A., Jones, A. Daniel, Barry, Cornelius S.“…Here, we use a combination of reverse genetics with discovery metabolomics and multivariate statistical analysis followed by in planta transient assays to investigate alkaloid diversity and functionally characterize two candidate cytochrome P450s genes from Atropa belladonna without a priori knowledge of their functions or information regarding the identities of key pathway intermediates. …”
Publicado 2022
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4155por Cechin, Inês, da Silva, Laura Prado, Ferreira, Elisa Teófilo, Barrochelo, Sarah Corrêa, de Melo, Fernanda Pereira de Souza Rosa, Dokkedal, Anne Ligia, Saldanha, Luiz Leonardo“…Photosynthetic parameters were evaluated in planta, and leaves were harvested for chemical analysis of photosynthetic pigments, proline, and phenolic contents. …”
Publicado 2022
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4156“…Unique classes of AQs from fungi include halogenated and steroidal AQs, and absent from planta are of potential to explore for biological activity against urging diseases such as cancer and multidrug-resistant pathogens. …”
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4157por Gutkowska, Małgorzata, Kaus‐Drobek, Magdalena, Hoffman‐Sommer, Marta, Małgorzata Pamuła, Magdalena, Daria Leja, Anna, Perycz, Małgorzata, Lichocka, Małgorzata, Witek, Agnieszka, Wojtas, Magdalena, Dadlez, Michał, Swiezewska, Ewa, Surmacz, Liliana“…In line with this, a C‐terminal REP truncation does not have pronounced phenotypic effects in planta. On the contrary, a complete lack of functional REP leads to male sterility in Arabidopsis: pollen grains develop in the anthers, but they do not germinate efficiently and hence are unable to transmit the mutated allele. …”
Publicado 2021
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4158por Eastman, Samuel, Smith, Thomas, Zaydman, Mark A., Kim, Panya, Martinez, Samuel, Damaraju, Neha, DiAntonio, Aaron, Milbrandt, Jeffrey, Clemente, Thomas E., Alfano, James R., Guo, Ming“…Agrobacterium‐mediated transient expression and in planta inoculation assays were performed to determine HopAM1’s intrinsic enzymatic activity and virulence contribution. …”
Publicado 2021
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4159por Grech‐Baran, Marta, Witek, Kamil, Poznański, Jarosław T., Grupa‐Urbańska, Anna, Malinowski, Tadeusz, Lichocka, Małgorzata, Jones, Jonathan D. G., Hennig, Jacek“…Ry(sto) associates directly with PVY CP in planta that is conditioned by the presence of a CP central 149 amino acids domain. …”
Publicado 2022
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4160por Rattan, Usha Kumari, Kumar, Surender, Kumari, Reenu, Bharti, Monika, Hallan, Vipin“…The interaction between CMV 2b and HB27 proteins was further validated using in planta (bimolecular fluorescence complementation assay) and in vitro far-Western blotting (FWB) methods. …”
Publicado 2022
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