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  1. 20141
    “…PCR amplification and sequencing of the gene coding for 18, using primers developed for 3 and 1, identified the putative linear precursor protein of 18 as being comprised of the first three amino acid residues of 3 (MLI), four conserved amino acid residues of 3 and/or 1 (PPFF), and the last two residues of 1 (LI). …”
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    Novel flax orbitide derived from genetic deletion
  2. 20142
    “…Moreover, by direct sequencing with the same primers, we were able to evaluate the genotypes of rs179784 of KCNQ1 which shows strong linkage disequilibrium with rs179785 (D’ = 1.0 and r(2) = 0.99). rs11653176, a common variant of BCAS3, showed a significant association with gout (P = 1.66 × 10(− 3); odds ratio [OR] = 0.80); the direction of effect was the same as that seen in the previous Han Chinese GWAS. …”
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    Common variant of BCAS3 is associated with gout risk in Japanese population: the first replication study after gout GWAS in Han Chinese
  3. 20143
    “…The presence of maize chromatin was indicated in 66 lines by amplification of the PCR product (500 bp) generated using primers specific for the maize retrotransposon Grande-1. …”
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    Complex characterization of oat (Avena sativa L.) lines obtained by wide crossing with maize (Zea mays L.)
  4. 20144
    “…We sampled environmental DNA from multiple sites and designed new Diphyllatea-specific PCR primers for long-read PacBio RSII technology. Near full-length 18S rRNA sequences from environmental DNA, in addition to supplementary Diphyllatea sequence data mined from public databases, resolved the phylogeny into three deeply branching and distinct clades (Diphy I – III). …”
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    Enigmatic Diphyllatea eukaryotes: culturing and targeted PacBio RS amplicon sequencing reveals a higher order taxonomic diversity and global distribution
  5. 20145
    “…The presence of HPV DNA was firstly confirmed by HPV consensus PCR using PGMY09/PGMY11 designed primers, then HPV positive samples were further genotyped by the membrane hybridization method to detect the 21 high-risk HPV (HR-HPV) and low-risk HPV types. …”
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    Prevalence and type-specific distribution of human papillomavirus infection among women in mid-western rural, Nepal- A population-based study
  6. 20146
    “…PCR with cas-specific primers showed that 30.7% (54/176) of the clinical isolates had a CRISPR-Cas system. …”
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    Characterization of CRISPR-Cas Systems in Clinical Klebsiella pneumoniae Isolates Uncovers Its Potential Association With Antibiotic Susceptibility
  7. 20147
    “…All influenza A positive samples were further subtyped using primers and probes for A/H1N1pdm09 and A/H3 whereas influenza B samples were further subtyped into B/Yamagata and B/Victoria lineages. …”
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    The 2015 Outbreak of Severe Influenza in Kashmir, North India: Emergence of a New Clade of A/H1n1 Influenza Virus
  8. 20148
    “…The disc cells were isolated and incubated with IL-19 and IL-20 under CoCl(2)-mimicked hypoxic conditions to analyze the proinflammatory cytokine expression pattern using real-time quantitative PCR with specific primers. RESULTS: IL-19 and IL-20 were positively stained and accompanied by abundant expression of TNF-α, IL-1β, and MCP-1 in facet joints of DLS patients. …”
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    The roles of IL-19 and IL-20 in the inflammation of degenerative lumbar spondylolisthesis
  9. 20149
    “…The HLA loci were independently amplified by long-range PCR using previously designed HLA-locus specific primers and subsequently sequenced using SMRT and Ion PGM sequencers. …”
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    Reference Grade Characterization of Polymorphisms in Full-Length HLA Class I and II Genes With Short-Read Sequencing on the ION PGM System and Long-Reads Generated by Single Molecule, Real-Time Sequencing on the PacBio Platform
  10. 20150
    “…Molecular detection of genes encoding enterotoxin (bft) production was carried out using bftF and bftR primers. Subsets of bft-positive isolates were also investigated by sequencing and correlated to various sepsis. …”
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    655. Antimicrobial Susceptibility and Prevalence of Extra-intestinal Enterotoxigenic Bacteroides fragilis Among a 5-Year Collection of Isolates Causing Sepsis in Kuwait
  11. 20151
    “…METHODS: A novel multiplex qRT-PCR assay with intron-spanning primers was developed for the parallel quantification of FG and MG. …”
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    A multiplex assay for the sensitive detection and quantification of male and female Plasmodium falciparum gametocytes
  12. 20152
    “…Faecal samples were homogenized, and DNA was amplified by PCR using primers directed to the 16S bacterial rRNA gene. Pyrosequencing was performed using GS-Junior platform. …”
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    Modulation of faecal metagenome in Crohn’s disease: Role of microRNAs as biomarkers
  13. 20153
    “…In all studies the LAMP assay and qPCR primers were directed against the ctrA region of the Neisseria meningitidis bacteria. …”
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    A systematic review of the diagnostic accuracy of Loop-mediated-isothermal AMPlification (LAMP) in the diagnosis of invasive meningococcal disease in children
  14. 20154
    “…Strains were studied for their carriage of these genes by polymerase chain reaction, using specific primers, and 28 strains (30.8%) were found to carry at least one type of sul gene, mainly associated to a class 1 integron (17 strains), and identified by 16S rRNA gene sequencing as mainly belonging to the Pseudomonas genus (21 strains). …”
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    Occurrence of Transferable Integrons and sul and dfr Genes Among Sulfonamide-and/or Trimethoprim-Resistant Bacteria Isolated From Chilean Salmonid Farms
  15. 20155
    “…High throughput sequencing of small RNA (sRNA) combined with bioinformatics, and molecular identification based on PCR detection with virus-specific primers and DNA sequencing is a desirable approach to identify an unknown infectious agent. …”
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    Identification and characterization of water chestnut Soymovirus-1 (WCSV-1), a novel Soymovirus in water chestnuts (Eleocharis dulcis)
  16. 20156
    “…However, nothing is known about the prevalence of Tetraparvovirus in special livestock living on the Qinghai-Tibet Plateau of China, such as Tibetan pigs and Tibetan sheep. A pair of special primers was designed based on the conserved regions in the genome of ungulate tetraparvovirus 2 (P-PARV4) and ungulate tetraparvovirus 4 (O-PARV4) and was used to detect P-PARV4 in domestic pigs and Tibetan pigs and O-PARV4 in ovines and Tibetan sheep. …”
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    First detection and genetic characterization of ungulate tetraparvovirus 2 and ungulate tetraparvovirus 4 in special livestock on the Qinghai-Tibet Plateau in China
  17. 20157
    “…However, there are limited data on the apicoplast genomes of Babesia species infective to small ruminants. METHODS: PCR primers were designed based on the previously reported apicoplast genome sequences of Babesia motasi Lintan and Babesia sp. …”
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    Comparative analysis of apicoplast genomes of Babesia infective to small ruminants in China
  18. 20158
    “…To validate cassette PCR testing, conventional PCR using STEC primers was performed on each of the 820 samples. …”
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    Detection of pathogenic Escherichia coli on potentially contaminated beef carcasses using cassette PCR and conventional PCR
  19. 20159
    “…And 14 of 16 randomly selected circRNAs were experimentally validated with divergent primers. Our results showed that 186 circRNAs were significantly differentially expressed in WXS (F) compared with WXS (S), of which 97, 87 and 60 circRNAs were differentially expressed at the pollen mother cell (PMC) formation stage (P2), the meiosis stage (P3) and the microspore formation stage (P4), respectively. …”
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    Circular RNA profiling of the rice photo-thermosensitive genic male sterile line Wuxiang S reveals circRNA involved in the fertility transition
  20. 20160
    “…Viral RNA underwent PCR with primers targeting the core and envelope-1 protein (CE1) and sequenced via Sanger sequencing. …”
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    A23 Population level diversification of hepatitis C viral strains over time among people who inject drugs in Baltimore, MD
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