“…To improve specific binding of oligonucleotide primers and TaqMan probes, we used locked nucleic acid technology. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The tested biotinylated primers do not negatively affect the RT-qPCR pipeline and thus can be optionally applied to enable deeper inspection of RT-qPCR results by subsequent pyrosequencing. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Exon-3 GHR genotype was determined by PCR using previously described sense and antisense primers. Results: The cohort consisted of 28 patients, 54% female, with a mean age of 51 ± 12 years. …”
Enlace del recurso
Enlace del recurso

“…DNA was extracted from each blood sample, and a nested PCR was performed using general apicomplexan primers for the partial 18S rRNA gene. PCR products were electrophoresed in agarose matrix, and appropriately sized amplicons were sequenced. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Further development of within species- and genus-specific barcodes could contribute to designing PCR primers for efficient and accurate identification of N. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…METHODS: miRNA expression profiles were assessed in maternal serum (> 36 weeks’ gestation) from women delivering a severely FGR infant (defined as an individualised birthweight centile (IBC) < 3rd) and matched control participants (AGA; IBC = 20–80th), using miRNA arrays. qPCR was performed using specific miRNA primers in an expanded cohort of patients with IBC < 5th (n = 15 males, n = 16 females/group). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…DNA was extracted from dried blood spots by the Chelex-100 method and the Plasmodium falciparum status detected by nested PCR amplification of the 18SrRNA gene using specific predesigned primers. Of the 300 women enrolled, the proportion of malaria parasite infected as determined by microscopy, RDT and PCR was 12.9%, 16.4% and 29.4% respectively, with 39.9% overall infected with P. falciparum by microscopy and/or RDT and/or PCR and a very low-density infection, averaging 271 parasites per microliter of blood. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Furthermore, we also demonstrated the viability of using nearby regions to MADC2 with novel primers as alternative assays. Finally, we also show proof of concept of several additional commercially viable sex determination methodologies for Cannabis sativa. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The assays were based on Fast Virus (FV) and qScript (qS) chemistries using the same primers and probes, but at different concentrations and under different cycling conditions. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…DNA was extracted from samples, and the V4 region of the 16S rRNA gene was amplified with region‐specific primers. The 16S rRNA sequencing on an Illumina NovaSeq. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Circulating miRNAs were extracted from blood serum and profiled through real-time PCR on a commercial 384 well-array, containing spotted forward primers for 372 miRNAs. Data analysis was performed by using the online data analysis software GeneGlobe and normalized by the global Ct mean method. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Two groups of fish were used for infection experiments, and tissues from healthy goldfish were collected for RNA isolation. cDNA synthesis was performed, and primers were designed based on transcriptome database sequences. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In this study, the sequence of the sheep KAP22-2 gene (KRTAP22-2) was aligned into the goat genome, and the sequence with the highest homology was assumed to be the goat KRTAP22-2 sequence and used to design primers to amplify the goat gene sequence. A total of 356 Longdong Cashmere goats (Gansu Province, China) were used for screening of genetic variants. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…This work entailed the assay design and optimization for use on the automated, high-throughput (HTP) cobas® 5800/6800/8800 Systems, including primers/probes, sample processing, and result interpretation for three sample types (BAL, sputum, and positive blood culture). …”
Enlace del recurso
Enlace del recurso

“…RT-PCR with specific primers for each gene revealed that they have a differential tissue distribution which overlaps with the distribution of the single mammalian receptor. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…RESULTS: Taking advantage of the new orthologous DNA sequences available in locusts, we developed primers for genes encoding 18SrRNA, ribosomal protein L32 (RpL32), armadillo (Arm), actin 5C (Actin), succinate dehydrogenase (SDHa), glyceraldehyde-3P-dehydrogenase (GAPDH), elongation factor 1 alpha (EF1a) and annexin IX (AnnIX). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…SLC22A12 W258X was genotyped with a polymerase chain reaction with confronting two-pair primers. RESULTS: The genotype frequency was 4,793 for WW, 225 for WX, and 5 for XX, which was in Hardy-Weinberg equilibrium (p = 0.164) with X allele 0.023 (95% confidence interval [0.021-0.027]). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Based on the genome and transcriptome data, a set of core proteins was extracted, and primers were designed on two gene regions with a relatively low degree of conservation within sect. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Bovine TB lesions found at PM were not all due to M. bovis alone, as other MTBC and AFB organisms may cause bTB-like lesions that were excluded by PCR specific primers. The prevalence of bTB was higher in Bauchi abattoir that supplies larger population of the state with beef. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Total genomic DNA was extracted from these samples and used as template for PCR amplification using pfoB, gyrA and orf1 gene specific primers for diagnosis of T. vaginalis (TV), Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) respectively. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso