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  1. 18341
    “…Specific CRISPR RNAs (crRNAs) and primers are designed for Salmonella DNA and parC gene S80I mutation diagnosis. …”
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    Detection of Salmonella DNA and drug-resistance mutation by PCR-based CRISPR-lbCas12a system
  2. 18342
    “…Asymmetric isothermal recombinase polymerase amplification using ferrocene labeled forward primers was employed to generate single stranded redox labeled amplicons. …”
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    Semiautomated Electrochemical Melting Curve Analysis Device for the Detection of an Osteoporosis Associated Single Nucleotide Polymorphism in Blood
  3. 18343
    “…The captured mosquito samples were milled and screened with JEV-specific primers. Five viruses were isolated, FJ1901, FJ1902, FJ1903, FJ1904, and FJ1905. …”
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    Genotype Change in Circulating JEV Strains in Fujian Province, China
  4. 18344
    “…In this study, a quadruplex real-time quantitative RT-PCR (RT-qPCR) for the simultaneous detection of four porcine RVs was developed by designing specific primers and probes based on the VP6 gene of RVA, RVB, RVC, and RVH, respectively. …”
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    Development of a Quadruplex RT-qPCR for the Detection of Porcine Rotaviruses and the Phylogenetic Analysis of Porcine RVH in China
  5. 18345
    “…As well as SCoT molecular marker technique effectively induced molecular alterations between the treatments. Where, SCoT primers targeted many new and missing amplicons that are expected to be associated with the lowly and highly expressed genes with 18.2 and 81.8%, respectively. …”
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    Changes in morphological traits, anatomical and molecular alterations caused by gamma-rays and zinc oxide nanoparticles in spinach (Spinacia oleracea L.) plant
  6. 18346
    “…Next, nested PCRs with divergent primers were performed and the purified PCR products were subjected to 3rd generation nanopore sequencing. …”
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    Novel circular RNAs of the apoptosis‐related BAX and BCL2L12 genes identified in a chronic lymphocytic leukemia cell line using nanopore sequencing
  7. 18347
    “…A fragment in the domain II (S5-S6) of the VGSC gene was amplified using the specific primers and the resultant polymerase chain reaction products were sequenced. …”
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    Detection of pyrethroids resistance alleles in goat biting louse Bovicola caprae (Phthiraptera: Trichodectidae) in west and northwest of Iran
  8. 18348
    “…For the molecular detection of S. aureus and its virulence genes, the polymerase chain reaction (PCR) was performed with species-specific primers. The overall prevalence of S. aureus was found to be 40% (40/100), which was confirmed through molecular detection of the nuc gene in 40/40 (100%) of samples using PCR. …”
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    Occurrence of Virulence Genes among Methicillin-Resistant Staphylococcus aureus Isolated from Subclinical Bovine Mastitis
  9. 18349
    “…The aim of this work is to evaluate the variability of morphometric traits of sexually mature living female R. rosea plants and to conduct a comparative analysis of genetic variability in cenopopulations (CP) both under undisturbed conditions and under stressful conditions of anthropogenic impact (grazing). Of the 8 primers used, HB12 turned out to be the most informative. …”
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    Morphometric Characteristics and Genetic Issr Marker Variability in Rhodiola rosea L. (Crassulaceae) in Different Ecological and Geographic Conditions in the Altai Republic
  10. 18350
    “…The DRD2 genotype was determined by a new method, polymerase chain reaction with confronting two-pair primers (PCR-CTPP). In males, current smokers were 35.3% of individuals with A1A1 genotype, 43.1% of individuals with A1A2 genotype, and 57.0% of individuals with A2A2 genotype, while in females, they were 19.6%, 14.6%, and 10.9%, respectively. …”
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    Association between Smoking Habits and Dopamine Receptor D2 TaqI A A2 Allele in Japanese Males: a Confirmatory Study
  11. 18351
    “…Molecular identification was done using β-tubulin primers and ITS5 sequencing of 13 different isolates that releveled 98% homology with BLAST (GenBank accession no. …”
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    First identification of potato tuber rot caused by Penicillium solitum, its silver nanoparticles synthesis, characterization and use against harmful pathogens
  12. 18352
    “…Relative gene expression was then evaluated by qRT-PCR with specific primers for VISTA, Galectin-9, and TIM-3. Also, the mRNA expression profile and clinical data of 154 B-ALL patients were obtained from the TARGET. …”
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    Expression of Galectin-9-related immune checkpoint receptors in B-cell acute lymphoblastic leukemia
  13. 18353
    “…A total of 51 loci were initially identified, and locus-specific primers were designed and tested with 20 epidemiologically unrelated isolates. …”
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    The first established microsatellite markers to distinguish Candida orthopsilosis isolates and detection of a nosocomial outbreak in China
  14. 18354
    “…The percentage of positive BCT over the infection (30%) was lower than of positive PCR (56% and 62%, depending on primers). Among the serological tests, the percentage of positive tests was 97%, 96%, 86% and 84% for RDT, ELISA, immune trypanolysis and CATT, respectively. …”
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    Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs
  15. 18355
    por Hyun, Miri, Lee, Ji Yeon, Kim, Hyun ah
    Publicado 2023
    “…Capsular serotypes and virulence factors were identified by polymerase chain reaction using specific primers. RESULTS: A total 79 patients were analyzed, 39 (49.4%) of 2014-2015 and 40 (50.6%) of 2020-2021. …”
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    654. Changing trends of molecular characteristics and antimicrobial susceptibility of hypervirulent Klebsiella pneumoniae in community-acquired liver abscess before and after COVID-19 outbreak in South Korea
  16. 18356
    “…HAstV was identified using pan-astrovirus consensus primers by Reverse transcription PCR. RESULTS: During the study period, 3.3% (49/1,482) of specimens were identified as HAstV, with a detection rate of 2.5% (37/1,482) for classic HAstV and 0.8% (12/1,482) for novel HAstV. …”
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    Diversity of classic and novel human astrovirus in outpatient children with acute gastroenteritis in Shanghai, China
  17. 18357
    “…Furthermore, specific primers were used for singleplex PCR targeting BRGs (cepA, qacA, and qacE) and efflux pump (norA, norB, norC, sepA, mepA and mdeA) associated genes. …”
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    Molecular distribution of biocide resistance genes and susceptibility to biocides among vancomycin resistant Staphylococcus aureus (VRSA) isolates from intensive care unit (ICU) of cardiac hospital- A first report from Pakistan
  18. 18358
    “…The presence of HPV was detected using nested PCR including MY09/11 primers and sequencing were performed for virus genotyping. …”
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    Low presence of papillomavirus and its lack of correlation with clinicopathological factors in breast cancer: a case control study
  19. 18359
    “…Here, we investigated the efficiency of RAISING (Rapid Amplification of Integration Site without Interference by Genomic DNA contamination) as a simple and inexpensive method to detect viral integration by amplifying HBV-integrated fragments using virus-specific primers covering the entire HBV genome. METHODS AND RESULTS: Illumina sequencing of RAISING products from HCC-derived cell lines (PLC/PRF/5 and Hep3B cells) identified HBV-human junction sequences as well as their frequencies. …”
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    A versatile method to profile hepatitis B virus DNA integration
  20. 18360
    “…PCR amplification of pol and tax regions, using HTLV-1, -2 and STLV-3 highly conserved primers, demonstrated the presence of a new human retrovirus in one DNA sample. tax (180 bp) and pol (318 bp) phylogenetic analyses demonstrated the strong relationships between the novel human strain (Pyl43) and STLV-3 isolates from Cameroon. …”
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    Discovery of a new human T-cell lymphotropic virus (HTLV-3) in Central Africa
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