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  1. 18361
    “…Using sequences obtained from the three APN cDNA fragments and degenerate primers for a fourth APN (APN3), the full length sequences of four S. exigua APN cDNAs were obtained. …”
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    Bacillus thuringiensis Cry1Ca-resistant Spodoptera exigua lacks expression of one of four Aminopeptidase N genes
  2. 18362
    “…FasL expression was detected at the mRNA level by RT-PCR, using intron spanning primers, and at the protein level by Western Blotting and immunofluorescence, using a polyclonal FasL- specific antibody. …”
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    Fas ligand expression in human and mouse cancer cell lines; a caveat on over-reliance on mRNA data
  3. 18363
    “…RNAs isolated from male reproductive tract tissues of rat were reverse transcribed and PCR amplified using gene specific primers for defensins. PCR products were sequenced to confirm their identity. …”
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    Identification, cloning and functional characterization of novel beta-defensins in the rat (Rattus norvegicus)
  4. 18364
    por Garrick, Ryan C, Sunnucks, Paul
    Publicado 2006
    “…Based on a multiple allele alignment, locus-specific primers are designed in conserved regions, minimizing 'null' alleles. …”
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    Development and application of three-tiered nuclear genetic markers for basal Hexapods using single-stranded conformation polymorphism coupled with targeted DNA sequencing
  5. 18365
    “…In each assay, the probes for both normal and mutant alleles were combined in a single reaction. Multiple ERCA primers combined with a quenched-peptide nucleic acid (Q-PNA) fluorescent detection system greatly accellerated the appearance of signal. …”
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    High accuracy genotyping directly from genomic DNA using a rolling circle amplification based assay
  6. 18366
    “…CONCLUSION: The results show a high proportion of DEC among Tanzanian children with diarrhea, with typical EAEC and typical EPEC predominating. The use of primers for both variants of ST1 (st1a and st1b) increased the sensitivity for detection of ETEC strains.…”
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    Identification of diarrheagenic Escherichia coli isolated from infants and children in Dar es Salaam, Tanzania
  7. 18367
    “…Sequence changes were also introduced into some of the primers to improve the melting characteristics of the amplicon. …”
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    High resolution melting for mutation scanning of TP53 exons 5–8
  8. 18368
    por Chen, Y., Chew, C. T., Chan, S. H.
    Publicado 1995
    “…A panel of 18 V alpha- and 21 V beta-specific primers was used to detect the TCR gene use from cDNA. …”
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    T-cell receptor gene expression in tumour-infiltrating lymphocytes and peripheral blood lymphocytes of patients with nasopharyngeal carcinoma.
  9. 18369
    “…Thereafter, 95 colorectal cancer patients and a control group including healthy subjects (n=11), patients with other gastrointestinal neoplasms (n=11) or inflammatory bowel disease (n=9) were analysed. Specific cDNA primers for CEA transcripts were used to apply RT-PCR to peripheral blood samples. …”
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    Detection of colonic cells in peripheral blood of colorectal cancer patients by means of reverse transcriptase and polymerase chain reaction.
  10. 18370
    “…Upon perfect target hybridization, the PRI-lock probes are circularized via enzymatic ligation, subsequently serving as template for individual, standardized amplification via unique probe-specific primers. Adaptation to OpenArrays™, which can accommodate up to 3072 33 nl PCR amplifications, allowed high-throughput real-time quantification. …”
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    Quantitative multiplex detection of plant pathogens using a novel ligation probe-based system coupled with universal, high-throughput real-time PCR on OpenArrays™
  11. 18371
    “…Here, we surveyed its occurrence in a collection of 165 marine isolates belonging to abundant marine phyla using conserved degenerated PCR primers and sequencing of selected positive bands to determine if the presence of the luxS gene is phylogenetically conserved or dependent on the habitat. …”
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    Potential for luxS related signalling in marine bacteria and production of autoinducer-2 in the genus Shewanella
  12. 18372
    “…The oligonucleotide microarray was composed of eight probes including negative control and positive control from 16S rDNA gene. The six primers were designed to amplify target fragments in two triplex PCR, and then hybridized with oligonucleotide microarray. …”
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    Detection and identification of enterohemorrhagic Escherichia coli O157:H7 and Vibrio cholerae O139 using oligonucleotide microarray
  13. 18373
    “…Using RT-PCR and FISH, a series of metastases from 25 known cutaneous melanomas and 8 melanoma cell lines (5 uveal and 3 cutaneous) were screened for the t(12;22)(q13;q13) translocation. Primers for RT-PCR were chosen based upon published breakpoint sequences. …”
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    Malignant melanoma is genetically distinct from clear cell sarcoma of tendons and aponeurosis (malignant melanoma of soft parts)
  14. 18374
    “…KLK5 was amplified by PCR using gene specific primers, and the identity of the PCR product was verified by sequencing. …”
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    Human kallikrein gene 5 (KLK5) expression is an indicator of poor prognosis in ovarian cancer
  15. 18375
    por Tandle, A T, Sanghvi, V, Saranath, D
    Publicado 2001
    “…Hence, in the current study, we investigated p53 exon 4 codon 72 polymorphism using Proline or Arginine specific primers from the peripheral blood cells (PBC) representing constitutional DNA from 72 oral cancer patients. …”
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    Determination of p53 genotypes in oral cancer patients from India
  16. 18376
    “…The sequence was rapidly determined using a previously described approach that utilized a PCR-based method and employed universal primers, designed on the scaffold of multiple sequence alignment of chloroplast genomes. …”
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    Comparative chloroplast genomics and phylogenetics of Fagopyrum esculentum ssp. ancestrale – A wild ancestor of cultivated buckwheat
  17. 18377
    “…METHODS: P. falciparum field samples from 161 volunteers were genotyped by nested PCR using polymorphic MSP2 family-specific primers. Data were analysed to determine variability of band size measurements between identical MSP2 alleles randomized into different agarose lanes. …”
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    Distinction of Plasmodium falciparum recrudescence and re-infection by MSP2 genotyping: A caution about unstandardized classification criteria
  18. 18378
    “…The serological phenotype of the 3D7(SM )parasites was determined by flow cytometry using malaria semi-immune and immune plasma and transcription of the 59 var genes in 3D7 were analysed by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) using var-specific primers. RESULTS: A selection-induced increased adhesion of 3D7(SM )iRBC to CD36 resulted in a reduced var4 transcription and VAR4 surface expression. …”
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    CD36 selection of 3D7 Plasmodium falciparum associated with severe childhood malaria results in reduced VAR4 expression
  19. 18379
    “…CONCLUSION: Simulation revealed that sequential digestion of immobilized cDNA followed by the partitioning of released fragments into mutually exclusive pools outperformed other cDNA-AFLP protocols in terms of coverage, redundancy, fragment length, and the total number of PCRs. Primers generating 30 to 70 amplicons per PCR provided the highest fraction of electrophoretically distinguishable fragments suitable for normalization. …”
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    Improved coverage of cDNA-AFLP by sequential digestion of immobilized cDNA
  20. 18380
    “…RESULTS: By analyzing the nucleotide sequences of the genes for O-antigen biosynthesis including wba operon and the central variable regions of the H1 and H2 flagellin genes in Salmonella, designated PCR primers for four multiplex PCR reactions were used to detect and differentiate Salmonella serogroups A/D1, B, C1, C2, or E1; H1 antigen types i, g, m, r or z(10); and H2 antigen complexes, I: 1,2; 1,5; 1,6; 1,7 or II: e,n,x; e,n,z(15). …”
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    Rapid screening of Salmonella enterica serovars Enteritidis, Hadar, Heidelberg and Typhimurium using a serologically-correlative allelotyping PCR targeting the O and H antigen alleles
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