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  1. 18541
    “…DNA from abscess specimens was subjected to polymerase chain reaction amplification using 16S rRNA gene-specific primers and Cy3-dCTP labeling. Labeled DNA was then applied to microbial microarrays (280 species) generated by the Human Oral Microbial Identification Microarray Laboratory (Forsyth Institute, Cambridge, MA). …”
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    Oral microbiota species in acute apical endodontic abscesses
  2. 18542
    “…We screened 29 populations of P. patens using a set of six microsatellite primers. The results of our study indicate that the analyzed populations are characterized by low levels of genetic diversity (H(o) = 0.005) and very high levels of inbreeding (F(IS) = 0.90). …”
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    Genetic Diversity and Population Structure of the Rare and Endangered Plant Species Pulsatilla patens (L.) Mill in East Central Europe
  3. 18543
    “…We here present an alternative molecular approach to deal with these degradation phenomena by using internal Alu subfamily specific primers producing short fragments of approximately 150 bp. …”
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    Alu SINE analyses of 3,000-year-old human skeletal remains: a pilot study
  4. 18544
    por Bu, Jiaodi, Zhao, Jin, Liu, Mengjun
    Publicado 2016
    “…This study provides appropriate reference genes and corresponding primers for further RT-qPCR studies in Chinese jujube and emphasizes the importance of validating reference genes for gene expression analysis under variable experimental conditions.…”
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    Expression Stabilities of Candidate Reference Genes for RT-qPCR in Chinese Jujube (Ziziphus jujuba Mill.) under a Variety of Conditions
  5. 18545
    “…Seven Malaysian HRV-C complete genomes were obtained with newly redesigned primers. The seven genomes were classified as HRV-C6, C12, C22, C23, C26, C42, and pat16 based on the VP4/VP2 and VP1 pairwise distance threshold classification. …”
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    Comparative Genetic Analyses of Human Rhinovirus C (HRV-C) Complete Genome from Malaysia
  6. 18546
    “…Existing methods for direct molecular detection of microbial DNA in blood are limited in either sensitivity (likely the result of small sample volumes) or in breadth of coverage, often because the PCR primers and probes used target only a few specific pathogens. …”
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    The IRIDICA BAC BSI Assay: Rapid, Sensitive and Culture-Independent Identification of Bacteria and Candida in Blood
  7. 18547
    por Shanmuganathan, R, Kumaresan, R, Giri, P
    Publicado 2015
    “…MATERIALS AND METHODS: Blood samples were collected and subjected to biochemical analyses and nested PCR amplification was performed to genotype the DNA, for ACE I/D using specific primers. STATISTICAL ANALYSIS: Statistical analyses were performed using SPSS version 13. …”
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    Prevalence of angiotensin converting enzyme (ACE) gene insertion/deletion polymorphism in South Indian population with hypertension and chronic kidney disease
  8. 18548
    “…The SbNHX1 gene encodes a vacuolar Na(+)/H(+) antiporter and is involved in the compartmentalization of excess Na(+) ions into the vacuole and maintenance of ion homeostasis Transgenic cumin plants were confirmed by PCR using gene (SbNHX1, uidA and hptII) specific primers. The single gene integration event and overexpression of the gene were confirmed by Southern hybridization and competitive RT-PCR, respectively. …”
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    In planta Transformed Cumin (Cuminum cyminum L.) Plants, Overexpressing the SbNHX1 Gene Showed Enhanced Salt Endurance
  9. 18549
    “…The dynamics of parasite infection was determined by daily counting the number of eggs per gram of feces from day 1 to 28 post-infection. A set of primers for LAMP assay based on a DNA partial sequence in the 18S rRNA gene from S. venezuelensis was designed. …”
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    Strong-LAMP: A LAMP Assay for Strongyloides spp. Detection in Stool and Urine Samples. Towards the Diagnosis of Human Strongyloidiasis Starting from a Rodent Model
  10. 18550
    “…PCR was used for each strain of MDR using specific primers. RESULTS: In the current study, 131 A. baumannii isolates (22.3%) out of 588 were studied. …”
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    Beta-Lactamase Encoded Genes bla(TEM) and bla(CTX) Among Acinetobacter baumannii Species Isolated From Medical Devices of Intensive Care Units in Tehran Hospitals
  11. 18551
    “…Each reaction uses a combination of primers and probes designed to separate taxa through discriminatory annealing. …”
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    A Multi-Species TaqMan PCR Assay for the Identification of Asian Gypsy Moths (Lymantria spp.) and Other Invasive Lymantriines of Biosecurity Concern to North America
  12. 18552
    “…Methylation status in the promoter region was detected by methylation- and non-methylation-specific primers. Corresponding mRNA levels were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). …”
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    Correlations of Promoter Methylation in WIF-1, RASSF1A, and CDH13 Genes with the Risk and Prognosis of Esophageal Cancer
  13. 18553
    “…RESULTS: Specific PCR primers were designed to produce two amplified fragments for B-positive samples and the control fragment for B-negative samples. …”
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    Development of chromosomal markers based on next-generation sequencing: the B chromosome of the cichlid fish Astatotilapia latifasciata as a model
  14. 18554
    “…Samples seroreactive to anti-HEV were further tested for the presence of HEV RNA using nested polymerase chain reaction (PCR) with universal primers for detection of all four HEV genotypes. Positive PCR samples were then subjected to DNA sequencing for further analysis. …”
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    Detection of Hepatitis E Virus Genotype 1 Among Blood Donors From Southwest of Iran
  15. 18555
    “…We amplified a partial 16S ribosomal RNA gene (V1–V3 region) by polymerase chain reaction with specific primers. Our analysis revealed that whitefly infestation reshaped the overall microbiota structure compared to that of the control rhizosphere, even after 1 week of infestation. …”
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    Aboveground Whitefly Infestation-Mediated Reshaping of the Root Microbiota
  16. 18556
    “…Then a set of PCR reactions for exon1 of BMP-15 gene was performed using specific primers followed by genotyping with direct sequencing. …”
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    Mutation analysis of exon1 of bone morphogenetic protein-15 gene in Iranian patients with polycystic ovarian syndrome
  17. 18557
    “…The molecular characterization of E. histolytica or E. dispar was carried out by Polymerase Chain Reaction (PCR) using species-specific primers to amplify short tandem repeats (STR) in non-coding sequences associated with the tRNA gene; the amplified fragments were sequenced and analyzed. …”
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    Entamoeba histolytica and Entamoeba dispar infection in Mexican school children: genotyping and phylogenetic relationship
  18. 18558
    “…Chromosome number from growing root tip was examined following standard protocols. Twenty-six ISSR primers that generated polymorphic bands were used for genetic diversity analysis of the thirty accessions of cardamom. …”
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    Intraspecific variations in cardamom (Elettaria cardamomum Maton): assessment of genomic diversity by flow cytometry, cytological studies and ISSR analysis
  19. 18559
    “…For species differentiation, multiplex-PCR using 12.7 kb primers was conducted. Out of 28 PSLN aspirate, Mycobacterium bovis was detected in 18 (64.28%) and MTB in 8 (28.57%), whereas 2 aspirate samples (7.14%) were positive for both the species. …”
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    Evaluation of complementary diagnostic tools for bovine tuberculosis detection in dairy herds from India
  20. 18560
    “…CONCLUSIONS: We present a rapid and convenient random mutagenesis method that does not require laborious steps such as ligation, cloning, and synthesis of specific primers. This method was successfully applied to the protein expression system in P. pastoris. …”
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    Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris
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