“…RESULTS: Two targeted AHPND positive V. parahaemolyticus strains were confirmed using PCR with 16S rRNA, ldh, AP3 and AP4 primers. The assembled genomes of strain MSR16 and MSR17 were comprised of a total of 5,393,740 bp and 5,241,592 bp, respectively. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…METHODS: Serovar-specific primers and probes were designed based on a comparison of gene targets (wzx and wzy encoding for somatic antigen biosynthesis; fliC and fljB for flagellar antigens) from 5868 Salmonella genomes. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Wide variations were observed in the RILs for eleven morpho-physiological traits involved during the stress. A total of 401 SSR primers were surveyed for parental polymorphism of which 77 were detected to be polymorphic. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Thus, we developed various tetraplex and triplex quantitative PCR (qPCR) assays for X. fastidiosa detection and subspecies identification in planta in a single reaction. We designed primers and probes using SkIf, a bioinformatics tool based on k-mers, to detect specific signatures of the species and subspecies from a data set of 58 genome sequences representative of X. fastidiosa diversity. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Peritoneal fluid was collected at the time of surgery and processed for Next Generation Sequencing (NGS) using 16S V4 exon bacterial primers and bioinformatics analyses. We found that patients with OC had a unique peritoneal microbial profile compared to patients with a benign mass. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The amplification of β-lactamase genes bla(SHV), bla(TEM), bla(CTX-M), bla(OXA-48), bla(KPC), and bla(NDM), classes 1, 2, and 3 integrase genes, was carried out using specific primers and polymerase chain reaction (PCR) RESULTS: Of the 250 Klebsiella outpatient isolates, 3.6% were K. oxytoca and the rest were K. pneumoniae. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We designed different combinations of primers and dual-labeled hydrolysis probes located in various CAR regions. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The DNA of 90 fish was extracted from the caudal fins using a DNA extraction kit, after which it was amplified using primers Fish-F1 and Fish-R1. Sequencing was conducted by Applied Biosystems Macrogen Korea, and the DNA sequences were then edited and aligned using MEGA v. 7. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The amplicon-based HTS approach is a target enrichment method that relies on the amplification of a specific target using particular primers before sequencing. Thus, the results are highly dependent on the quality of amplification. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…PCR amplification of 16S rRNA genes was performed with universal primers amplifying the V4 variable region (515F-806R). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…A multiplex polymerase chain reaction (mPCR) assay was developed to detect and distinguish feline panleukopenia virus (FPV), feline bocavirus (FBoV) and feline astrovirus (FeAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of the three viruses and were used to specifically amplify targeted fragments of 237 bp from the VP2 gene of FPV, 465 bp from the NP1 gene of FBoV and 645 bp from the RdRp gene of FeAstV. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Thus, three variant IBVs of novel Italy-02 genotype, which had 96.7–99.2% S1 gene nucleotide identity with each other, belonged to three separate subgroups based on N gene sequences. 624/I-type isolate Italy-06 together with Italy-03, which was undetectable using S1 gene primers, shared 97.7% and 99.3% identity, respectively, in N gene region with vaccine strain H120. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…RT-PCRs were performed on viral RNAs to amplify S1 gene using a specific set of primers S1OLIGO3′ and S1OLIGO5′. Restriction polymorphism (RFLP) of PCR products was determined by the use of HaeIII restriction enzyme. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…However, pairwise comparisons of the predicted amino acid sequences of the 10 RbtOV and SttOV proteins with orthologs from representatives of the established orthomyxoviral genera and a phylogenetic analysis using the PB1 protein showed that while RbtOV and SttOV clustered most closely with ISAV, they diverged sufficiently to merit consideration as representatives of a novel genus. A set of PCR primers was designed using conserved regions of the PB1 gene to produce amplicons that may be sequenced for identification of similar fish orthomyxoviruses in the future.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…A droplet digital TaqMan probe-based qPCR (ddPCR) assay using methylation-specific primers and probes was employed to quantify unmethylated and methylated sequences. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The samples were established as C. trachomatis using real-time PCR (RT-PCR) with Cryptic F/Cryptic R primers. RESULTS: Of 150 positive HPV findings, the most common (72.7%, 109/150) were the 12 other HR HPVs (viz., 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…After further analyses and manual inspection, primers and probes for the optimum candidate genes were designed and tested in silico, for validation in future wet-lab studies. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…To determine the genetic mismatches between a donor and a recipient, we have implemented a real-time PCR method in conjunction with allele-specific primers (AS-qPCR). The new approach allows for multiplexing up to 480 reactions per 96 well plate and differs from common qPCR based genotyping methods. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The results of visual inspection were comparable to those obtained by agarose gel electrophoresis. The LAMP primers specifically amplified H. canis DNA, whereas no amplification was detected in DNA samples of other haemoparasites and no-template control revealing specificity of the assay. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The worm was isolated by necropsy and genomic DNA was extracted and partial cytochrome c oxidase subunit 1 gene was amplified using specific primers. Phylogenetic and Templeton-Crandall-Sing (TCS) network analyses were carried out on the sequence data. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso