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  1. 18681
    “…Thus, PCR analyses amplifying a partial T-DNA fragment with AtFT-specific primers are sufficient to determine whether the seedlings are transgenic or not. …”
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    Sequencing of two transgenic early-flowering poplar lines confirmed vector-free single-locus T-DNA integration
  2. 18682
    “…Samples that are avian influenza virus-positive were isolated in embryonated chicken eggs, and the subtypes were identified by RT-PCR with the specific primers. The samples positive for influenza virus H5 were sequenced to identify HA and NA genes. …”
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    The Presence of Poultry Influenza Strains in Two Live Bird Markets near the East-West Boundary of Vietnam
  3. 18683
    “…Objectives: Development and validation of a single-step and accurate reverse transcriptase loop-mediated isothermal amplification technique (RT-LAMP) for rapid identification of SARS-CoV-2 relative to commercial quantitative reverse transcriptase real-time PCR (qRT-PCR) assays to allow prompt initiation of proper medical care and containment of virus spread. Methods: Primers showing optimal in-silico features were subjected to analytical sensitivity and specificity to assess the limit of detection (LOD) and cross-reaction with closely- and distantly-related viral species, and clinically prominent bacterial and fungal species. …”
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    Development and Validation of a Rapid, Single-Step Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) System Potentially to Be Used for Reliable and High-Throughput Screening of COVID-19
  4. 18684
    “…HLA and KIR genotyping was performed using polymerase chain reaction with sequence-specific primers and/or sequence-specific oligonucleotides. …”
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    Maternal killer-cell immunoglobulin-like receptors and paternal human leukocyte antigen ligands in recurrent pregnancy loss cases in Turkey
  5. 18685
    “…DNA from 171 mother‐child pairs (342 samples) was studied for: (a) HLA‐G allele information by the NGSgo‐AmpX HLA‐G assay, (b) 3′UTR haplotype information by an in‐house developed sequence‐based typing method of a 699/713 base pair region in the 3′UTR, and (c) the full phase HLA‐G gene sequence, by combining primers from both assays. The mother to child inheritance allowed internal verification of newly identified alleles and of association between coding and UTR regions. …”
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    HLA‐G whole gene amplification reveals linkage disequilibrium between the HLA‐G 3′UTR and coding sequence
  6. 18686
    “…HIV genomes were generated from N = 13 plasma specimens by next-generation sequencing of metagenomic libraries prepared with spiked primers targeting HIV, followed by Sanger gap-filling. …”
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    New Genomes from the Congo Basin Expand History of CRF01_AE Origin and Dissemination
  7. 18687
    por Dorlass, Erick Gustavo, Monteiro, Cairo Oliveira, Viana, Amanda Oliveira, Soares, Camila Pereira, Machado, Rafael Rahal Guaragna, Thomazelli, Luciano Matsumiya, Araujo, Danielle Bastos, Leal, Fabyano Bruno, Candido, Erika Donizette, Telezynski, Bruna Larotonda, Valério, Camila Araujo, Chalup, Vanessa Nascimento, Mello, Ralyria, Almeida, Flavia Jaqueline, Aguiar, Andressa Simões, Barrientos, Anna Carlotta Mott, Sucupira, Carolina, De Paulis, Milena, Sáfadi, Marco Aurélio Palazzi, Silva, Daniella Gregorio Bonfim Prado, Sodré, Janaina Joice Martins, Soledade, Mariana Pereira, Matos, Samantha Faria, Ferreira, Sabrina Rodrigues, Pinez, Célia Miranda Nunez, Buonafine, Carolina Palamin, Pieroni, Leticia Nery Ferreira, Malta, Fernanda Mello, Santana, Rubia Anita Ferraz, Souza, Eloisa Corrêa, Fock, Ricardo Ambrosio, Pinho, João Renato Rebelo, Ferreira, Luís Carlos Souza, Botosso, Viviane Fongaro, Durigon, Edison Luiz, Oliveira, Danielle Bruna Leal
    Publicado 2020
    “…The objective of this study was to standardize lower cost molecular methods for SARS-CoV-2 identification. E gene primers previously determined for TaqMan assays by Colman et al. (2020) were adapted in SYBR Green assay and RT-PCR conventional. …”
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    Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR
  8. 18688
    “…The current study undertakes the assessment of the genetic variation and population structure of Anagallis foemina L. specimens collected in south-eastern Poland, Volhynian Polesie and West Volhynian Upland based on inter-simple sequence repeats (ISSR) polymorphism. Twenty ISSR primers amplified 374 DNA fragments, of which 79% were polymorphic. …”
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    Genetic diversity and population structure of endangered plant species Anagallis foemina Mill. [Lysimachia foemina (Mill.) U. Manns & Anderb.]
  9. 18689
    “…Selective amplification of target nucleic acid from the samples was achieved by using primers for ORF1a/b non-structural region and the structural protein envelope E-gene of the virus. …”
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    Post-mortem viral dynamics and tropism in COVID-19 patients in correlation with organ damage
  10. 18690
    “…We developed barcoded primers and a real-time analysis platform that generate accurate VP1 consensus sequences from multiplexed samples. …”
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    Rapid and Sensitive Direct Detection and Identification of Poliovirus from Stool and Environmental Surveillance Samples by Use of Nanopore Sequencing
  11. 18691
    “…The cDNA of all positive donors was then analyzed for the presence of various HIV genotypes (types and subtypes) by employing subtype-specific primers in a nested polymerase chain reaction. The amplified products were run on ethidium bromide-stained 2% agarose gel and visualized using a ultraviolet transilluminator. …”
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    Genetic Diversity of Human Immunodeficiency Virus Type 1 in Asymptomatic Blood Donors in Islamabad, Pakistan
  12. 18692
    “…Fresh sputum samples were collected for culturing and conducted polymerase chain reaction using BL-specific primers. Among the 101 patients recruited, presence of BL strains could be ascertained in 94 patients of which 24 (26%; 95% CI: 18%–35%) had BL strains. …”
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    Is International Travel an Emerging Issue on Transmission of Beijing Lineage Mycobacterium tuberculosis?
  13. 18693
    “…We found 780 genotypes and 324 alleles via the 19 microsatellites primers, and the results showed that the mean number of alleles (N(a)) was 17.05; the average polymorphism information content (PIC) was 0.767; the mean expected heterozygosity (He) was 0.662; as for observed heterozygosity (H(o)), it was 0.647. …”
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    Genetic Variation of Nine Chicken Breeds Collected from Different Altitudes Revealed by Microsatellites
  14. 18694
    “…To develop a species‐specific PCR primers were designed on the sequenced PCR amplicon from the M. mycetomatis specific PCR. …”
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    Diagnostic implications of mycetoma derived from Madurella pseudomycetomatis isolates from Mexico
  15. 18695
    “…Preliminary polymerase chain reaction screening of six isolates obtained from wheat blast infected samples with diagnostic primers (MoT3F/R) was conducted at ZARI, Zambia, and subsequent analysis of two isolates with MoT3F/R and C17F/R was performed at USDA-ARS, USA. …”
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    Detection and characterization of fungus (Magnaporthe oryzae pathotype Triticum) causing wheat blast disease on rain-fed grown wheat (Triticum aestivum L.) in Zambia
  16. 18696
    “…The sequence of PCR amplicons showed 100% identity and significant alignment with V. parahaemolyticus. The primers used provided high specificity for V. parahaemolyticus in PCR detection compared with another Vibrio species. …”
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    Diagnosis, genetic variations, virulence, and toxicity of AHPND-positive Vibrio parahaemolyticus in Penaeus monodon
  17. 18697
    “…The RT-PCR tests were performed with primers and probes targeting the RdRp gene (Bioexen LTD, Turkey) and results were quantified as cycle threshold (Ct) values. …”
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    Relationship of the cycle threshold values of SARS-CoV-2 polymerase chain reaction and total severity score of computerized tomography in patients with COVID 19
  18. 18698
    “…Overlap polymerase chain reaction (PCR) using different primers for 5ˈ and 3ˈ end of each segment produced fusion segment 1 + 2 and (1 + 2) +3 fragments and was used for cloning. …”
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    PlpE Epitopes of Pasteurella multocida Fusion Protein as Novel Subunit Vaccine Candidates
  19. 18699
    “…The objective of this study was to firstly develop and apply a CPA assay with propidium monoazide (PMA) for the rapid detection of the foodborne E. coli O157:H7 in VBNC state. Five primers (2a/1s, 2a, 3a, 4s, and 5a) were specially designed for recognizing three targets, which were rfbE, stx1, and stx2, and evaluated for its effectiveness in detecting VBNC cell of E. coli O157:H7 with detection limits of pure VBNC culture at 10(3), 10(5), and 10(5) colony-forming units (CFUs)/ml for rfbE, stx1, and stx2, respectively, whereas those of food samples (frozen pastry and steamed bread) were 10(3), 10(5), and 10(5) CFUs/ml. …”
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    Development and Application of a Simple “Easy To Operate” Propidium Monoazide-Crossing Priming Amplification on Detection of Viable and Viable But Non-culturable Cells of O157 Escherichia coli
  20. 18700
    “…Using a combination of high-throughput sequencing, abutting primers-based PCR recovery of circular genomic elements, cloning, and Sanger sequencing, we detected 97 novel sequences comprising 40 ssDNA viral genomes and 57 viral-like circular molecules from 45 individual penguins. …”
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    Identification and Distribution of Novel Cressdnaviruses and Circular Molecules in Four Penguin Species in South Georgia and the Antarctic Peninsula
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