“…Long-range PCR was peformed to validate the speculated CYP11B1 rearrangements with the breakpoint-specifc primers. RESULTS: Using the optimized target sequencing approach, we detected two intragenic/intergenic deletions of CYP11B1 and one chimeric CYP11B2/CYP11B1 gene from three suspected patients with 11β-OHD besides three pathogenic heterozygous point mutation/indels. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Symptomatic samples were tested for the presence of fig mosaic virus (FMV), fig leaf mottle-associated virus 1 (FLMaV-1), fig leaf mottle-associated virus 2 (FLMaV-2), fig mild mottle-associated virus (FMMaV), fig latent virus 1 (FLV-1), fig fleck-associated virus (FFkaV), and fig cryptic virus (FCV) using reverse transcription-polymerase chain reaction (RT-PCR) with specific primers. Three viruses were detected in mixed infections and showed positive results. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…This study evaluated 100 Sporothrix spp. strains obtained from 1999 to 2018 from the Evandro Chagas National Institute of Infectious Diseases, Fiocruz, which were identified through a polymerase chain reaction using specific primers for species identification. Two-fold serial dilutions of stock solutions of amphotericin B, itraconazole, posaconazole, ketoconazole and terbinafine prepared in dimethyl sulfoxide were performed to obtain working concentrations of antifungal drugs ranging from 0.015 to 8.0 mg/L. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…DNA amplif ication using original primers SCA1_3f and SCA1_3r from genomic DNA and restriction by endonuclease HindII made it possible to distinguish the SCA alleles coding for protein products with different charges without sequencing the gene. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Initially, available primers detecting Omicron-related mutations were rapidly validated in a central laboratory. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Isolates with DNA concentrations >0.9 ng/µl and confirmed PCR‐compatibility (n = 135) were subsequently screened for the presence of BPV13 DNA using BPV13‐specific PCR primers for amplification of a 771 bp region comprising the BPV13 E5 gene. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…For molecular analysis, the i n t e r n a l t r a n s c r i b e d s p a c e r 1 gene of E. tenella was amplified using species‐specific primers and sequenced. After editing and alignment, 263 bp sequences were used for analysis. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we devised a novel approach termed a multiple cross displacement amplification integrated with gold nanoparticle-based lateral flow biosensor (MCDA-AuNPs-LFB) for the highly specific, sensitive, user-friendly, and rapid identification of C. trachomatis in clinical samples. A suite of MCDA primers based on the C. trachomatis ompA gene from 14 serological variants (serovar A-K, L1, L2, and L3) were successfully designed and used to establish the assay. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…METHODS: DNA extracted from 53 cheese samples were analyzed by nested PCR with C. burnetii-specific primers and the products confirmed by DNA sequencing. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…A one-step RT-PCR assay, based on the use of a single set of specific primers, was used to retro-transcribe and amplify the entire IV type A genome in a single reaction, thus avoiding additional enrichment approaches and host genome removal treatments. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…DNA was extracted from whole blood samples of all patients and sequence-specific primers (SSP)-based polymerase chain reaction (PCR) was used for genotyping. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We tested the performance of two OceanDNA analysis methods—species-specific qPCR (quantitative polymerase chain reaction) and MiFish metabarcoding using universal primers—to determine the distribution of small pelagic fish in the open ocean. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Herein, we developed a rapid, minimally invasive, and convenient nucleic acid biomarker recognition method, which enabled the combined specific detection of 11 lung cancer typing markers in a microliter reaction system after only one sampling. The primers for the combined specific detection of 11 lung cancer typing markers were designed and screened, and the microfluidic chip for parallel detection of the multiple markers was designed and developed. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We then performed experimental validation of a subset of 100 PIP primers on eight pomegranate genotypes and identified 76 polymorphic markers, with 15 having PIC values ≥0.50. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…RESULTS: Eventually, 200 strains were isolated and 103 strains were further identified through the internal transcribed spacer (ITS, ITS1 and ITS2 regions) sequence by using the universal primers ITS5 and ITS4. A total of 59,106 fungal sequences corresponding to 374 putative operational taxonomic units (OTU) were identified by 454 pyrosequencing. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…METHODS: Samples from 91 patients with thalassemia were used for HCV genotyping by Cobas 4800 platform, and 50 cases of 1a, 1b, and 3a genotypes underwent amplification and sequencing of NS5A and NS5B by using consensus primers via conventional reverse transcription-polymerase chain reaction (RT-PCR) method. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The modified Hodge test confirmed the CRACB phenotype, and specific PCR primers were used for the molecular identification of bla(OXA) and bla(NDM) genes. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…DNA extraction was done using the phenol-chloroform method and ITS region was amplified using pan-fungal primers followed by Sanger sequencing. RESULTS: Among total of 22 Schizophyllum commune cultures, 19 were isolated from lower respiratory samples, two from corneal scrapings, and one from the brain. …”
Enlace del recurso
Enlace del recurso

“…The amplification enzymes and primers initiate the reaction within the dried blood matrix through these networks, precluding any need for conventional nucleic acid purification. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…METHODS: Reverse-transcription LAMP (RT-LAMP) assay primers were designed to detect envelop (E) and nucleocapsid (N) genes of SARS-CoV-2. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso