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  1. 18861
    “…These non-polyadenylated RNAs resisted RNAse R digestion and could be PCR amplified using outward-facing primers, suggesting they represent circular RNA structures that could arise from by-products of mRNA splicing. …”
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    Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
  2. 18862
    “…A comprehensive evaluation of Helicobacter genus and H. pylori PCR primers was also undertaken. The combined approach was then assessed in a range of gastrointestinal samples prior to assessment of a UC cohort. …”
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    Enterohepatic Helicobacter in Ulcerative Colitis: Potential Pathogenic Entities?
  3. 18863
    “…In attempts to determine the geographical distribution and variability of CpCSV, a pair of degenerate primers derived from conserved domains of the luteovirus coat protein (CP) gene was used for RT-PCR analysis of various legume samples originating from five countries and containing unidentified luteoviruses. …”
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    Molecular, serological and biological variation among chickpea chlorotic stunt virus isolates from five countries of North Africa and West Asia
  4. 18864
    “…RESULTS: Detection of all Campylobacter DNA utilising genus specific primers was significantly higher in cases of UC, with a prevalence of 73.9% (51/69) compared to 23.1% (15/65) in controls (p = 0.0001). …”
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    Detection of Campylobacter concisus and Other Campylobacter Species in Colonic Biopsies from Adults with Ulcerative Colitis
  5. 18865
    “…RESULTS: The two sulfotransferase genes were coded as Ixosc SULT 1 & 2 and corresponding proteins were referred as Ixosc Sult 1 and 2. Using gene-specific primers, the sulfotransferase transcripts were detected throughout the blacklegged tick life cycle, including eggs, larvae, nymphs, adult salivary glands and adult midgut. …”
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    Molecular characterization of novel sulfotransferases from the tick, Ixodes scapularis
  6. 18866
    “…In this study, we have developed a one-step, single tube, reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for rapid and sensitive detection of EV71. METHODS: Six primers that can recognize 6 distinct regions on the VP2 gene of EV71 were designed for RT-LAMP assay. …”
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    Development and evaluation of reverse transcription-loop-mediated isothermal amplification assay for rapid detection of enterovirus 71
  7. 18867
    “…The reverse transcriptase polymerase reactions using degenerate primers to carla-, potex- and poty-viruses linked viral RNA with colour break and sequencing of the amplified products indicated that the potexvirus Narcissisus mosaic virus was the predominant virus associated with the occurrence of the colour break. …”
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    Colour break in reverse bicolour daffodils is associated with the presence of Narcissus mosaic virus
  8. 18868
    por Jain, Swati, Ponmariappan, S., Kumar, Om
    Publicado 2011
    “…METHODS: The truncated fragment of the botulinum neurotoxin type B from amino acid 1-450 was synthesized using PCR overlap primers; the constructed gene was cloned in the pQE30UA vector and transformed to Escherichia coli SG 13009. …”
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    Development of immunodetection system for botulinum neurotoxin type B using synthetic gene based recombinant protein
  9. 18869
    “…Comparative whole genome mutational mapping of two PT13a strains that varied in the ability to contaminate eggs and to form biofilm was achieved using a high-density tiling platform with primers designed from a PT4 reference genome. Confirmatory Sanger sequencing was used on each putative SNP identified by mutational mapping to confirm its presence and location as compared to the reference sequence. …”
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    Single nucleotide polymorphisms that differentiate two subpopulations of Salmonella enteritidis within phage type
  10. 18870
    “…The multiplex assay shows specificity for vervet DNA within the determined allele range for vervet monkeys; however, the primers will also amplify human DNA for each marker resulting in amplicons outside the vervet allele range in several of the loci. …”
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    Authentication of African green monkey cell lines using human short tandem repeat markers
  11. 18871
    “…Probes were obtained from 300-483 bp-long inserts of plasmid clones and from 506-559 bp-long fragments amplified with primers designed using the An. nili genome assembly generated on an Illumina platform. …”
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    Improving the population genetics toolbox for the study of the African malaria vector Anopheles nili: microsatellite mapping to chromosomes
  12. 18872
    “…Although we obtained high quality sequences with the suggested primers, species discrimination in our data set was only 43.48%. …”
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    DNA Barcoding Bromeliaceae: Achievements and Pitfalls
  13. 18873
    “…RESULTS: 49 samples (45.37%) were identified as Streptococcus iniae, 37 samples (35.2%) matched with Lactococcus garvieae; and 22 samples (19.43%) were identified as members of Streptooccus genus by culture-based and biochemical tests of API 50 CH, API 20 STREP and rapid 32 STREP systems. Using universal primers for differentiation of Streptococcus sp. and Enterococcus sp, all 108 samples were identified as Streptococcus sp. with a target region of 500 bp. …”
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    Molecular epidemiology of zoonotic streptococcosis/lactococcosis in rainbow trout (Oncorhynchus mykiss) aquaculture in Iran
  14. 18874
    “…Consistent with the binding assay, reverse transcriptional polymerase chain reaction using voltage-dependent anion channel–specific primers detected the K5R mRNA in the Müller cells. …”
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    Decreased K5 receptor expression in the retina, a potential pathogenic mechanism for diabetic retinopathy
  15. 18875
    “…METHODOLOGY/PRINCIPAL FINDINGS: In this study, we demonstrated that the key factors based on the bioinformatic analysis for selecting target sequence are the higher genome proportion, repetitive complete copies and partial copies, and active ESTs than the others in the chromosome genome. New primers based on 25 novel retrotransposons and SjR2 were designed and their sensitivity and specificity for detecting S. japonicum DNA were compared. …”
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    Sensitive and Specific Target Sequences Selected from Retrotransposons of Schistosoma japonicum for the Diagnosis of Schistosomiasis
  16. 18876
    “…After reverse-transcription, we performed multiplex PCR using primers for the genes ga733.3, muc-1 and c-erbB2. …”
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    Gene expression of circulating tumour cells in breast cancer patients
  17. 18877
    “…Samples were tested by Real time reverse transcriptase PCR using primers and probes developed at CDC, Atlanta for four influenza target genes. …”
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    Emergence of pandemic 2009 influenza A H1N1, India
  18. 18878
    “…Odorant receptors (ORs) were cloned using degenerate primers, and tested with the Xenopus oocyte expression system. …”
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    Moth Sex Pheromone Receptors and Deceitful Parapheromones
  19. 18879
    “…In addition, in ∼2.4% of samples pVL was dramatically underestimated by TaqMan due to poor binding of the proprietary TaqMan primers. CONCLUSIONS: The replacement of Amplicor with the TaqMan assay has altered the previously accepted definition of HIV treatment failure without any evidence to support the clinical relevance of the new definition. …”
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    Technical and Regulatory Shortcomings of the TaqMan Version 1 HIV Viral Load Assay
  20. 18880
    “…Two different adult blood fed mosquitoes had a single mermithid nematode emerge from their anus while they were being held post-capture. Primers from the 18 S rDNA were developed to sequence nematode DNA and screen mosquitoes for mermithid DNA. 18 S rDNA from the Senegalese mermithid and other mermithid entries in GenBank were used to create a Maximum Parsimony tree of the Mermithidae family. …”
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    Mermithid nematodes found in adult Anopheles from southeastern Senegal
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