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  1. 18881
    “…Molecular identification was performed using selective nifH-g1 primers. Alginate production and nitrogenase activity assay by each isolate of Azotobacter were carried out. …”
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    Correlation between nitrogen fixation rate and alginate productivity of an indigenous Azotobacter vinelandii from Iran
  2. 18882
    “…Methylation-specific primers were designed to interrogate two or more CpG in the same assay. …”
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    Development of a Quantitative Methylation-Specific Polymerase Chain Reaction Method for Monitoring Beta Cell Death in Type 1 Diabetes
  3. 18883
    “…Based on GenBank sequence datasets of 115 rodent species, a 136 bp fragment of cytochrome b was selected as the most discriminatory mini-barcode, and rodent universal primers surrounding this fragment were designed. The efficacy of this new molecular tool was assessed on 946 samples including rodent tissues, feces, museum samples and feces/pellets from predators known to ingest rodents. …”
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    Next-Generation Sequencing for Rodent Barcoding: Species Identification from Fresh, Degraded and Environmental Samples
  4. 18884
    “…We tested for an association between an intronic TGC trinucleotide repeat in TCF4 and FECD by determining repeat length in 66 affected participants with severe FECD and 63 participants with normal corneas in a 3-stage discovery/replication/validation study. PCR primers flanking the TGC repeat were used to amplify leukocyte-derived genomic DNA. …”
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    A Common Trinucleotide Repeat Expansion within the Transcription Factor 4 (TCF4, E2-2) Gene Predicts Fuchs Corneal Dystrophy
  5. 18885
    “…Intron based PCR was was used to amplify transcripts of PBMC pre-mRNA in which intron-5 had been removed (mRNA) while another set of primers was used to amplify intron 5-containing pre-transcripts (pre-mRNA). …”
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    286 Association Analysis of Member RAS Oncogene Family Gene Polymorphisms with Aspirin Intolerance in Asthmatic Patients
  6. 18886
    “…Odc1 genotyping was conducted using primers that amplify a 172-bp fragment containing the polymorphic base at +316. …”
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    Meat consumption, ornithine decarboxylase gene polymorphism, and outcomes after colorectal cancer diagnosis
  7. 18887
    “…DNA was extracted from bacteria-infected and uninfected control cultures, and primers specific for Rickettsia 16S rRNA, ompB, and sca4 genes were used to generate PCR products that were subsequently sequenced. …”
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    Tick cell culture isolation and growth of Rickettsia raoultii from Dutch Dermacentor reticulatus ticks
  8. 18888
    “…PCR amplification with primers directed outwards from the tet(S/M) gene identified the presence of a 4.3 kb circular form corresponding to the intervening chromosomal region between the two IS1216 elements, but lacking a replication origin. …”
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    Molecular characterization of a novel mosaic tet(S/M) gene encoding tetracycline resistance in foodborne strains of Streptococcus bovis
  9. 18889
    “…For qPCR-MN, infected MDCK-London cells in 96-well cell-culture plates are processed with minimal steps such that resulting samples are amenable to high-throughput analysis by downstream one-step quantitative reverse transcription PCR (qRT-PCR; SYBR Green chemistry with primers targeting a conserved region of the M1 gene of influenza A viruses). …”
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    Development of a Neutralization Assay for Influenza Virus Using an Endpoint Assessment Based on Quantitative Reverse-Transcription PCR
  10. 18890
    “…To understand more about PvLCY-β and PvCHY-β, both strands of both cDNA clones were sequenced using M13 forward and reverse primers. Nucleotide and deduced protein sequences were analyzed and annotated using online bioinformatics tools. …”
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    Computational analysis of common bean (Phaseolus vulgaris L., genotype BAT93) lycopene β-cyclase and β-carotene hydroxylase gene's cDNA
  11. 18891
    “…Genomic DNA was isolated and genotyped using PCR assay for the IL-4 gene 70 bp VNTR polymorphism determined by using PCR with the specific primers. RESULTS: There was statistical significance between the groups regarding IL-4 genotype distribution (p<0.001, odds ratio: 2.55 [1.629–4.052], 95% confidence interval) and allele frequencies (p<0.0012.381[1.586–3.617], 95% confidence interval). …”
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    Association of IL-4 gene VNTR variant with deep venous thrombosis in Behçet’s disease and its effect on ocular involvement
  12. 18892
    por Chen, Guo-Lin, Miller, Gregory M.
    Publicado 2013
    “…We performed a comprehensive analysis of alternative splicing (AS) of REST by rapid amplification of cDNA ends and PCR amplification of cDNAs from various tissues and cell lines with specific primers. We identified 8 novel alternative exons including an alternate last exon which doubles the REST gene boundary, along with numerous 5′/3′ splice sites and ends in the constitutive exons. …”
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    Extensive Alternative Splicing of the Repressor Element Silencing Transcription Factor Linked to Cancer
  13. 18893
    “…Plasmid replicon typing was performed by multiplex-PCR and sequencing with specific primers for 18 rep-families and 1 unique sequence. …”
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    Characterisation of the Plasmidome within Enterococcus faecalis Isolated from Marginal Periodontitis Patients in Norway
  14. 18894
    “…In this study we quantify the abundance and prevalence of the wasting disease pathogen among 19 Z. marina populations in northern European coastal waters, using quantitative PCR (QPCR) with primers targeting a species specific portion of the internally transcribed spacer (ITS1) of L. zosterae. …”
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    Quantitative PCR Reveals Strong Spatial and Temporal Variation of the Wasting Disease Pathogen, Labyrinthula zosterae in Northern European Eelgrass (Zostera marina) Beds
  15. 18895
    “…The constant high quality of sequences generated imparts confidence in extending the application of this non-purified amplicon sequencing approach to other gene sequencing assays, with appropriate use of suitably designed primers.…”
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    Simplified Large-Scale Sanger Genome Sequencing for Influenza A/H3N2 Virus
  16. 18896
    “…The DNA sequence alignments are provided and can be used for the design of additional primers.…”
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    Identification and Differentiation of Verticillium Species and V. longisporum Lineages by Simplex and Multiplex PCR Assays
  17. 18897
    “…In agreement with these findings, a set of hmuY gene-based primers and standard/real-time PCR with SYBR Green chemistry allowed us to specifically detect P. gingivalis in patients with chronic periodontitis (77.3%) and healthy subjects (20%), the latter possessing lower number of P. gingivalis cells and total bacterial cells. …”
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    The Unique hmuY Gene Sequence as a Specific Marker of Porphyromonas gingivalis
  18. 18898
    por Saini, Chaman, Ramesh, V., Nath, Indira
    Publicado 2013
    “…METHODOLOGY/PRINCIPLE FINDINGS: An array of Th17 related primers for cytokines, chemokines and transcription factors was used in real time reverse transcribed PCR to evaluate gene expression, ELISA for cytokine secretion in the supernatants of antigen stimulated PBMC cultures and flow cytometry for establishing the phenotype of the IL-17, IL-21 producing cells. …”
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    CD4+ Th17 Cells Discriminate Clinical Types and Constitute a Third Subset of Non Th1, Non Th2 T Cells in Human Leprosy
  19. 18899
    “…A sequence from C. oncophora (Con-pgp) was identified, showing 83 % similarity to Pgp-9 of Caenorhabditis elegans. Primers specific to putative Con-pgp-9 mRNA were designed, generating a 153-bp PCR product. …”
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    PGP expression in Cooperia oncophora before and after ivermectin selection
  20. 18900
    “…To address this, our Functional Gene Pipeline and Repository (FunGene; http://fungene.cme.msu.edu/) offers databases of many common ecofunctional genes and proteins, as well as integrated tools that allow researchers to browse these collections and choose subsets for further analysis, build phylogenetic trees, test primers and probes for coverage, and download aligned sequences. …”
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    FunGene: the functional gene pipeline and repository
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