Materias dentro de su búsqueda.
Mostrando 19,041 - 19,060 Resultados de 20,464 Para Buscar '"primer"', tiempo de consulta: 0.27s Limitar resultados
  1. 19041
    “…RESULTS: Using degenerative primers and the rapid amplification of cDNA ends (RACE) procedure, we cloned the Duplex-Specific Nuclease (DSN) gene from the hepatopancreas of the Kamchatka crab and determined its full primary structure. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    Isolation, characterization and molecular cloning of Duplex-Specific Nuclease from the hepatopancreas of the Kamchatka crab
  2. 19042
    “…METHODS: Methylation-specific primers were designed based on the methylation profiles of the TGFBI promoter in human tumor cell lines, and MSP conditions were optimized for accurate and efficient amplification. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    Methylation screening of the TGFBI promoter in human lung and prostate cancer by methylation-specific PCR
  3. 19043
    “…First, we designed, tested and optimized amplification primers for real-time RT-PCR. Then, expression data from each candidate gene were evaluated with three complementary approaches based on different statistical procedures. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process
  4. 19044
    “…Efficiency of the treatment was estimated by quantitative polymerase chain reaction and Fluorescent in situ hybridization (FISH), using Blochmannia specific primers (qPCR) and two fluorescent probes (one for all Eubacterial and other specific for Blochmannia). …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    Blochmannia endosymbionts improve colony growth and immune defence in the ant Camponotus fellah
  5. 19045
    “…The gene-to-oligo algorithm of the Gene Design Module plans out all of the required overlapping oligonucleotides and mutagenic primers needed to synthesize the desired gene constructs by PCR, and for physically cloning them into selected vectors by the most popular subcloning strategies. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    Gene Composer: database software for protein construct design, codon engineering, and gene synthesis
  6. 19046
    “…Bacterial DNA was extracted from the specimens and PCR analyses were carried out to detect 10 selected periodontopathic species using specific primers for each. In addition, statistical analyses were performed to analyze the correlations among clinical parameters and the detected species. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    Distribution of periodontopathic bacterial species in Japanese children with developmental disabilities
  7. 19047
    “…In this study, we used consensus astrovirus primers targeting the RNA polymerase to define the diversity of astroviruses present in pediatric patients with diarrhea on two continents. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    Human stool contains a previously unrecognized diversity of novel astroviruses
  8. 19048
    “…Real-time PCR (RT-PCR) has become the standard method to detect and quantify virus infections, including enteroviruses, in clinical samples. For instance, primers directed to the highly conserved 5' untranslated region (5'UTR) of the enterovirus genome enable detection of a wide spectrum of enteroviruses. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR
  9. 19049
    “…Real-time PCR was performed with specific primers for transcript I [GenBank:DQ131799] and II [GenBank:DQ131800]. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    Variable expression of cysteinyl leukotriene type I receptor splice variants in asthmatic females with different promoter haplotypes
  10. 19050
    “…BACKGROUND: DNA signatures are distinct short nucleotide sequences that provide valuable information that is used for various purposes, such as the design of Polymerase Chain Reaction primers and microarray experiments. Biologists usually use a discovery algorithm to find unique signatures from DNA databases, and then apply the signatures to microarray experiments. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    A parallel and incremental algorithm for efficient unique signature discovery on DNA databases
  11. 19051
    “…PCR amplification with genus-specific primers resulted in production of a ~2170 bp DNA band, which is similar to that of D. salina 18S rDNA gene containing only one intron near 5' terminus. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    Introduction of a novel 18S rDNA gene arrangement along with distinct ITS region in the saline water microalga Dunaliella
  12. 19052
    “…The entire cohort was screened by RT-PCR (with HA gene primers) using nasopharyngeal swabs three times a week. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    Seroconversion and asymptomatic infections during oseltamivir prophylaxis against Influenza A H1N1 2009
  13. 19053
    “…The real-time PCR using Scorpion primers was performed in all cases to assess clarithromycin primary resistance and point mutations involved. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    Primary resistance to clarithromycin, metronidazole and amoxicillin of Helicobacter pylori isolated from Tunisian patients with peptic ulcers and gastritis: a prospective multicentre study
  14. 19054
    “…Viruses of the 2.2.1v lineage represent drift variants escaping from conventional vaccine-induced immunity and some of these strains also escaped detection by commercial real time reverse transcriptase PCR (RT-qPCR) protocols due to mismatches in the primers/probe binding sites. RESULTS: We developed therefore a versatile, sensitive and lineage-specific multiplex RT-qPCR for detection and typing of H5N1 viruses in Egypt. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    Simultaneous detection and differentiation by multiplex real time RT-PCR of highly pathogenic avian influenza subtype H5N1 classic (clade 2.2.1 proper) and escape mutant (clade 2.2.1 variant) lineages in Egypt
  15. 19055
    “…When applying our sequence data to evaluate published MgPa operon-based diagnostic PCR assays and genotyping systems, we found that 11 of 19 primers contain up to 19 variable nucleotides and that the target for one of two typing systems is located in a hypervariable repeat region, suggesting the likelihood of false results with some of these assays. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    Genetic Variation in the Complete MgPa Operon and Its Repetitive Chromosomal Elements in Clinical Strains of Mycoplasma genitalium
  16. 19056
    “…The incorporation and differentiation of Houbara gPGCs in chimeric chicken testis were assessed by PCR with Houbara-specific primers and 31.3% (5/16) gonads collected from the injected chicken embryos showed the presence of donor Houbara cells. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    Primordial Germ Cell-Mediated Chimera Technology Produces Viable Pure-Line Houbara Bustard Offspring: Potential for Repopulating an Endangered Species
  17. 19057
    “…A unique combination of barcoded primers and a partitioned sequencing plate was employed to designate each sequence read to its original sample. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Texto
    Detection and mapping of mtDNA SNPs in Atlantic salmon using high throughput DNA sequencing
  18. 19058
    “…Members of the Culex pipiens complex were separated by measuring the ratio of the dorsal and ventral arms (DV/D ratio) of the male genitalia and also by using diagnostic primers designed for the Ace.2 gene. Culex pipiens quinquefasciatus was the most abundant form collected. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Online Artículo Texto
    The distribution of potential West Nile virus vectors, Culex pipiens pipiens and Culex pipiens quinquefasciatus (Diptera: Culicidae), in Mexico City
  19. 19059
    “…Total RNA was extracted and reverse transcribed before amplification in a TaqMan qPCR reaction using primers and probes specific for MIG (CXCL9), IP-10 (CXCL10) and HPRT. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Online Artículo Texto
    A whole blood monokine-based reporter assay provides a sensitive and robust measurement of the antigen-specific T cell response
  20. 19060
    “…These sites are introduced using synthetic DNA primers or are naturally present in the sequence used. …”
    Enlace del recurso
    Enlace del recurso
    Enlace del recurso
    Online Artículo Texto
    Enzymatic synthesis of long double-stranded DNA labeled with haloderivatives of nucleobases in a precisely pre-determined sequence
Herramientas de búsqueda: RSS