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  1. 19201
    “…Through extensive sequence analyses using 418 genomes, specific primers and probes were designed for a total of 22 antigen gene targets for the O- and K- serogroups. …”
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    Simultaneous Identification of Clinically Common Vibrio parahaemolyticus Serotypes Using Probe Melting Curve Analysis
  2. 19202
    “…For blood meal identification, we used a molecular assay based on cloning and sequencing following PCR amplification with vertebrate universal primers, and allowing the detection of multiple blood meals in a single bug. …”
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    Disentangling Trypanosoma cruzi transmission cycle dynamics through the identification of blood meal sources of natural populations of Triatoma dimidiata in Yucatán, Mexico
  3. 19203
    por Pauvolid-Corrêa, Alex, Gonçalves Dias, Helver, Marina Siqueira Maia, Laura, Porfírio, Grasiela, Oliveira Morgado, Thais, Sabino-Santos, Gilberto, Helena Santa Rita, Paula, Teixeira Gomes Barreto, Wanessa, Carvalho de Macedo, Gabriel, Marinho Torres, Jaire, Arruda Gimenes Nantes, Wesley, Martins Santos, Filipe, Oliveira de Assis, William, Castro Rucco, Andreza, Mamoru dos Santos Yui, Rafael, Bosco Vilela Campos, João, Rodrigues Leandro e Silva, Renato, da Silva Ferreira, Raquel, Aparecido da Silva Neves, Nilvanei, Charlles de Souza Costa, Michell, Ramos Martins, Leticia, Marques de Souza, Emerson, dos Santos Carvalho, Michellen, Gonçalves Lima, Marina, de Cássia Gonçalves Alves, Fernanda, Humberto Guimarães Riquelme-Junior, Luiz, Luiz Batista Figueiró, Luan, Fernandes Gomes de Santana, Matheus, Gustavo Rodrigues Oliveira Santos, Luiz, Serra Medeiros, Samara, Lopes Seino, Larissa, Hime Miranda, Emily, Henrique Rezende Linhares, José, de Oliveira Santos, Vanessa, Almeida da Silva, Stephanie, Araújo Lúcio, Kelly, Silva Gomes, Viviane, de Araújo Oliveira, Alexandre, dos Santos Silva, Julia, de Almeida Marques, William, Schafer Marques, Marcio, Junior França de Barros, José, Campos, Letícia, Couto-Lima, Dinair, Coutinho Netto, Claudia, Strüssmann, Christine, Panella, Nicholas, Hannon, Emily, Cristina de Macedo, Barbara, Ramos de Almeida, Júlia, Ramos Ribeiro, Karen, Carolina Barros de Castro, Maria, Pratta Campos, Larissa, Paula Rosa dos Santos, Ana, Marino de Souza, Isabelle, de Assis Bianchini, Mateus, Helena Ramiro Correa, Sandra, Ordones Baptista Luz, Renato, dos Santos Vieira, Ananda, Maria de Oliveira Pinto, Luzia, Azeredo, Elzinandes, Tadeu Moraes Figueiredo, Luiz, Augusto Fonseca Alencar, Jeronimo, Maria Barbosa de Lima, Sheila, Miraglia Herrera, Heitor, Dezengrini Shlessarenko, Renata, Barreto dos Santos, Flavia, Maria Bispo de Filippis, Ana, Salyer, Stephanie, Montgomery, Joel, Komar, Nicholas
    Publicado 2019
    “…We screened whole blood samples and mosquito pools for ZIKV RNA using pan-flavivirus primers in a real-time reverse-transcription polymerase chain reaction (RT-PCR) in a SYBR Green platform. …”
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    Zika Virus Surveillance at the Human–Animal Interface in West-Central Brazil, 2017–2018
  4. 19204
    “…Given the recognized role of Sphaerotilus in organically enriched environments, additional analyses were pursued to specifically characterize its abundance: a consensus sthA sequence was determined via comparison of whole metagenome sequences with a previously identified sthA sequence, the primers developed for this gene were used to characterize relative Sphaerotilus abundance using quantitative real-time PCR, and a Sphaerotilus strain was isolated to validate the determined sthA sequence. …”
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    Advanced biofilm analysis in streams receiving organic deicer runoff
  5. 19205
    “…METHODS: We amplified the entire mitochondrial (mt) genome of S. destruens in two overlapping long fragments using primers designed based on the cox1, cob and nad5 partial sequences. …”
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    The complete mitochondrial genome of a parasite at the animal-fungal boundary
  6. 19206
    “…SDS-PAGE profile of seed proteins generated 54 and 28 polymorphic bands and a total polymorphism of 62.06 and 41.17% in Gomati VU-89 and Pusa-578, respectively. SSR primers amplified a total of 16 and 24 polymorphic bands with an average polymorphism of 20.69 and 50.74% in Gomati VU-89 and Pusa-578, respectively. …”
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    Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers
  7. 19207
    “…PCR was also performed on 24 healthy control persons. The primers were specifically designed for this particular species of Borrelia. …”
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    Borrelia miyamotoi: 43 Cases Diagnosed in France by Real-Time PCR in Patients With Persistent Polymorphic Signs and Symptoms
  8. 19208
    “…Using a single digestion plus a sticky-end ligation step, the ccdB cassette that functions as a negative (counter) selection system is replaced by a pair of 28 nt self-annealing primers that provide specificity against the selected target miRNA/siRNA. …”
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    Protocol: low cost fast and efficient generation of molecular tools for small RNA analysis
  9. 19209
    “…The complete VP2 gene of these samples was amplified using VP2 specific primers and polymerase chain reaction (PCR). The obtained full-length VP2 sequences were analyzed and a phylogenetic tree was constructed. …”
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    Dynamic evolution of canine parvovirus in Thailand
  10. 19210
    “…From the evaluation of the analytical specificity (ASp) by in silico and in vitro approaches, it was congruently revealed that the primers and probes selected only recognized the specific targets for which the assay was intended. …”
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    A Validated Multiplex Real-Time PCR Assay for the Diagnosis of Infectious Leptospira spp.: A Novel Assay for the Detection and Differentiation of Strains From Both Pathogenic Groups I and II
  11. 19211
    “…In this study, we performed a survey to detect BToV in Japan in 2004 and 2005 using 231 fecal samples (167 from diarrheic cattle and 64 from asymptomatic cattle) that were analyzed by nested reverse transcription (RT) PCR using primers located in the consensus sequences of the reported BToV nucleocapsid (N), membrane (M), and spike (S) genes. …”
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    Epidemiological analysis of bovine torovirus in Japan
  12. 19212
    “…Toxoplasma gondii oocyst detection and an estimation of their numbers was performed by conventional PCR and real time qPCR, using specific primers for a 183-bp sequence of the T. gondii repetitive DNA region. …”
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    Detection of Toxoplasma gondii oocysts in fresh vegetables and berry fruits
  13. 19213
    “…Trypanosoma species were identified in tsetse from a subsample of 2184 using PCR. Primers specific for T. brucei s.l. and for T. brucei sub-species were used to screen cattle, pig and tsetse samples. …”
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    Evidence of the absence of human African trypanosomiasis in two northern districts of Uganda: Analyses of cattle, pigs and tsetse flies for the presence of Trypanosoma brucei gambiense
  14. 19214
    “…Polymerase chain reaction (PCR) was used to amplify the HPV sequence with generic primers. We detected specific HPV types using the INNO-LiPA HPV Genotyping Extra II Kit (INNOGENETICS). …”
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    High prevalence of human papillomavirus and European variants of HPV 16 infecting concomitantly to cervix and oral cavity in HIV positive women
  15. 19215
    “…After that, PCR, electrophoresis, and DNA sequencing were conducted on the extracted DNA based on COL1A1 and COL11A1 primers. RESULTS: The SNPs in COL1A1 are c.20980G/A in 27 patients and c.20980G>A in 8 patients, whereas SNPs in COL11A1 are both c.134373C/A and c.134555C/T in 8 patients and both c.[134373A>C] and c.134582G>A in 10 patients. …”
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    Single Nucleotide Polymorphisms (SNPs) of COL1A1 and COL11A1 in Class II Skeletal Malocclusion of Ethnic Javanese Patient
  16. 19216
    “…In this study we used the MinION to sequence long-range PCR amplicons from multiple dinoflagellate species with a focus on the genus Alexandrium. Primers applicable to a wide range of dinoflagellates were selected, meaning that although the study was primarily focused on Alexandrium the applicability to three additional genera of toxic algae, namely; Gonyaulax, Prorocentrum, and Lingulodinium was also demonstrated. …”
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    The Application of Nanopore Sequencing Technology to the Study of Dinoflagellates: A Proof of Concept Study for Rapid Sequence-Based Discrimination of Potentially Harmful Algae
  17. 19217
    “…The concentrations of CFD in sera were measured before and after treatment by quantitative real time PCR (qPCR) using primers of the human β-globin gene. RESULTS: At T0, all patients presented significant higher levels of ESR (P=0.05) and CFD (P=0.001) compared with controls. …”
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    Circulating Cell-Free DNA as Inflammatory Marker in Egyptian Psoriasis Patients
  18. 19218
    “…The accessions were also genotyped using a 15K Brassica SNP array and 60 PCR-based primers linked to previously identified clubroot resistance genes. …”
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    Genome-Wide Mapping of Loci Associated With Resistance to Clubroot in Brassica napus ssp. napobrassica (Rutabaga) Accessions From Nordic Countries
  19. 19219
    “…Here we design customized SWGA primers which successfully amplify P. malariae DNA extracted directly from unprocessed clinical blood samples obtained from patients with P. malariae-mono-infections from six countries, and further test the efficacy of SWGA on mixed infections with other Plasmodium spp. …”
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    Selective whole genome amplification of Plasmodium malariae DNA from clinical samples reveals insights into population structure
  20. 19220
    “…In this study, the specific primers for polymerase chain reaction (PCR) were designed based on the sequence of TvAP33 (GenBank Accession No. …”
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    The Molecular Characterization and Immunity Identification of Trichomonas vaginalis Adhesion Protein 33 (AP33)
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