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  1. 19341
    “…PCR using inter simple sequence repeat (ISSR) primers revealed 92% polymorphism among 14 tropical and temperate strains of B. mori, with average diversity index of 0.747. …”
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    Molecular Markers for Biomass Traits: Association, Interaction and Genetic Divergence in Silkworm Bombyx mori
  2. 19342
    “…For each candidate gene, haplotypes were identified and their frequencies in severely and moderately affected dogs were compared to detect a putative correlation between a gene-specific haplotype(s), and severity level of the disease. Primers were derived from expressed sequence tags (ESTs) and predicted transcripts to assess the relative retinal expression of the six genes of interest in normal and affected retinas of different ages. …”
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    Analysis of six candidate genes as potential modifiers of disease expression in canine XLPRA1, a model for human X-linked retinitis pigmentosa 3
  3. 19343
    “…METHODS: HCS cells, grown as primary and low-passage cultures of fibroblasts, were used as the sources of RNA for reverse transcriptase PCR, with primers specific for mRNAs encoding the proteins that comprise NADPH oxidases. …”
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    NADPH oxidase expression and production of superoxide by human corneal stromal cells
  4. 19344
    “…Repetitive sequences can be mapped using special molecular markers that are based on PCR with primers designed from two unique "repeat junctions". …”
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    JunctionViewer: customizable annotation software for repeat-rich genomic regions
  5. 19345
    “…In total, 190 ESTs differentially expressed between M.26 and G.41 were identified using 42 pairs of AFLP primers. cDNA-AFLP analysis of global EST expression in a resistant and a susceptible apple genotype identified different major classes of genes. …”
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    Identification of genes differentially expressed during interaction of resistant and susceptible apple cultivars (Malus × domestica) with Erwinia amylovora
  6. 19346
    “…We located and characterized thousands of simple sequence repeats and single nucleotide polymorphisms as potential molecular markers in our assembled and annotated sequences. High quality PCR primers were designed for a substantial number of the SSR loci, and a large number of these were amplified successfully in initial screening. …”
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    Transcriptome sequencing in an ecologically important tree species: assembly, annotation, and marker discovery
  7. 19347
    “…RESULTS: DNA amplicons from 1,710 rodent samples were individually barcoded using a combination of tags located in forward and reverse primers. Amplicons consisted in 222 bp fragments corresponding to DRB exon 2, a highly polymorphic gene in mammals. …”
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    A 454 multiplex sequencing method for rapid and reliable genotyping of highly polymorphic genes in large-scale studies
  8. 19348
    “…When microsatellites are isolated using standard SSR enrichment protocols and primers designed at complementary repeated regions, amplification from multiple genomic sites can cause scoring difficulties that compromise their utility as markers. …”
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    Generation of microsatellite repeat families by RTE retrotransposons in lepidopteran genomes
  9. 19349
    “…The NruI restriction endonuclease gene (nruIR) was cloned by PCR and inverse PCR using primers designed from the N-terminal amino acid sequence. …”
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    Cloning of NruI and Sbo13I restriction and modification sstems in E. coli and amino acid sequence comparison of M.NruI and M.Sbo13I with other amino-methyltransferases
  10. 19350
    “…RESULTS: Specific PCR primers and probes for the pathovars savastanoi, nerii and fraxini of P. savastanoi were designed to be used in End Point and Real-Time PCR, both with SYBR(® )Green or TaqMan(® )chemistries. …”
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    Development of a versatile tool for the simultaneous differential detection of Pseudomonas savastanoi pathovars by End Point and Real-Time PCR
  11. 19351
    “…Genomic DNA was extracted from peripheral blood lymphocytes of all the studied subjects. Universal primers were used to amplify four regions of the BRCA1 gene (exons 2,8,13 and 22) and one region (exon 9) of BRCA2 gene using specific PCR. …”
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    Presymptomatic breast cancer in Egypt: role of BRCA1 and BRCA2 tumor suppressor genes mutations detection
  12. 19352
    “…A method for genotyping and subtyping HBV by partial HBsAg gene sequencing using primers that are complementary to all known genotypes was used. …”
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    Hepatitis B virus genotypes and precore and core mutants in UAE patients
  13. 19353
    “…High risk (HR) HPV positive samples were typed using GP5+/GP6+ primed PCR, followed by Reverse Line Blot for 18 high/intermediate risk HPV types, while low risk (LR) HPV positive samples were tested with type specific primers for HPV6 and HPV11. RESULTS: 3817 women had a valid HCII test: 350 of them (9.2%) were positive for HR probes, 160 (4.2%) for LR probes, while 57 women were positive for both. …”
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    Prevalence of HPV high and low risk types in cervical samples from the Italian general population: a population based study
  14. 19354
    “…Reverse Transcription Reaction-Polymerase chain reaction (RT-PCR) tests were performed for specific GABA(A)R receptor subunit gene primers synthases of non-hibernating (NHIB) and hibernating (HIB) hamsters. …”
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    Distinct α subunit variations of the hypothalamic GABA(A )receptor triplets (αβγ) are linked to hibernating state in hamsters
  15. 19355
    “…The aim of the study was to compare HPV detection using SPF(10 )broad-spectrum primers PCR followed by DEIA and genotyping by LiPA(25 )(version 1) between freshly frozen cervical tissue samples and paraffin embedded blocks of cervical tissue from the same patient. …”
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    Comparison of human papillomavirus detection between freshly frozen tissue and paraffin embedded tissue of invasive cervical cancer
  16. 19356
    “…In addition, we evaluated DNA from 101 patients, isolated from blood cells, which was amplified by using PCR with sophisticated primers for P1 promoter of IGF-1 gene and IGF-1 receptor gene, then analyzed utilizing SSCP technique and automatically sequenced. …”
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    Are Elevated Levels of IGF-1 Caused by Coronary Arteriesoclerosis?: Molecular and Clinical Analysis
  17. 19357
    “…METHODS: Faecal microbiota of 13 UC patients, 11 IBS patients and 22 healthy volunteers were analysed by PCR-Denaturing Gradient Gel Electrophoresis (DGGE) using universal and Bacteroides specific primers. The data obtained were normalized using in-house developed statistical method and interrogated by multivariate approaches. …”
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    Ulcerative colitis and irritable bowel patients exhibit distinct abnormalities of the gut microbiota
  18. 19358
    “…Isolation, cloning, and sequencing analysis using cDNA-AFLP furnished 90 cDNA fragments with eight pairs of selective primers. Fifteen randomly selected genes were used for a combined virus-induced gene silencing (VIGS) knockdown experiment, using BaMV infection to investigate the roles played by these genes during viral infection, specifically addressing the means by which these genes influence the accumulation of BaMV protein. …”
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    Identification of differentially expressed genes induced by Bamboo mosaic virus infection in Nicotiana benthamiana by cDNA-amplified fragment length polymorphism
  19. 19359
    “…METHODS: A real-time quantitative PCR (rt-qPCR) method using primers and a hydrolysis probe that targets the 18S rRNA gene was adapted and optimized to estimate parasite load in blood samples. …”
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    A real-time, quantitative PCR method using hydrolysis probes for the monitoring of Plasmodium falciparum load in experimentally infected human volunteers
  20. 19360
    por Barathi, V.A., Beuerman, Roger W.
    Publicado 2011
    “…RT–PCR and northern blots were performed using specific primers for M(1)-M(5), and products sequenced. Real-time PCR was used to quantify message levels. …”
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    Molecular mechanisms of muscarinic receptors in mouse scleral fibroblasts: Prior to and after induction of experimental myopia with atropine treatment
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