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  1. 19581
    “…Subsequently, we developed a MethyLight assay using specific primers and probes for these CpG sites to detect the percentage of methylated reference (PMR) of the CDO1 promoter. …”
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    Targeted demethylation of the CDO1 promoter based on CRISPR system inhibits the malignant potential of breast cancer cells
  2. 19582
    “…However, badgers (164%), wild boars (0%), and corsacs (0%) presented lower invasion levels. Using specific primers, larvae samples were identified as Trichinella nativa. …”
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    Trichinellosis dissemination among wild carnivores in the Republic of Kazakhstan: A 10-year study
  3. 19583
    “…The genetic fidelity of 12 regenerated plants was analysed using RAPD primers that were genetically identical to the mother plant. …”
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    Plant regeneration through somatic embryogenesis in cell suspensions of Cenchrus ciliaris L.
  4. 19584
    “…DNA was extracted and bisulphite treated. PCR primers were designed to amplify 75–200 bp regions of the CpG rich gene promoters of p16, RARβ, E-cadherin, cytoglobin and cyclinA1. …”
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    Promoter methylation of P16, RARβ, E-cadherin, cyclin A1 and cytoglobin in oral cancer: quantitative evaluation using pyrosequencing
  5. 19585
    “…A public database containing genome-specific primers, SNPs, and other information was constructed. …”
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    Nucleotide diversity maps reveal variation in diversity among wheat genomes and chromosomes
  6. 19586
    “…When HPV16 and HPV18 type–specific primers were used, two specimens were positive for HPV16 at low copy number but were negative on additional type-specific HPV16 testing. …”
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    Assessment of Human Papillomavirus in Lung Tumor Tissue
  7. 19587
    “…Based on the identified SNP, three primers were designed to develop an ASM for dehydration tolerance. …”
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    Association of an SNP in a novel DREB2-like gene SiDREB2 with stress tolerance in foxtail millet [Setaria italica (L.)]
  8. 19588
    “…Harvested flies after every 24 hours were dissected and examined under a light microscope for trypanosome infections and whole fly DNA was extracted from 82 flies and analyzed for trypanosomes by polymerase chain reaction (PCR) using different sets of primers. RESULTS: The proportions of total tsetse catches per trap were in the following decreasing order S3 (33%), H-Trap (27%), Pyramidal (19%), sticky panel (11%) and biconical trap (10%). …”
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    Multiple Trypanosoma infections are common amongst Glossina species in the new farming areas of Rufiji district, Tanzania
  9. 19589
    “…Recently, we developed a new quantitative real-time polymerase chain reaction (PCR) method using Coordination of Common Motifs (CoCoMo) primers to measure the proviral load of known and novel BLV variants in BLV-infected animals. …”
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    BLV-CoCoMo-qPCR: a useful tool for evaluating bovine leukemia virus infection status
  10. 19590
    “…HPV DNA testing was undertaken using PCR with broad spectrum L1 consensus PGMY09/11 primers with genotyping of positive samples by Roche Linear Array. …”
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    Investigating a cluster of vulvar cancer in young women: a cross-sectional study of genital human papillomavirus prevalence
  11. 19591
    “…METHODS AND FINDINGS: CSF from patients with suspected meningitis from Salvador, Brazil were tested with culture, Gram stain, and real-time PCR using S. pneumoniae, N. meningitidis, and H. influenzae specific primers and probes. An antibiotic detection disk bioassay was used to test for the presence of antibiotic activity in CSF. …”
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    Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
  12. 19592
    “…No single locus was found to be the ideal DNA barcode gene for the genus, and species identification needs to be based on a combination of gene loci and morphological characters. Additional primers were developed to supplement those previously published for amplification of the loci used in this study. …”
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    Species concepts in Cercospora: spotting the weeds among the roses
  13. 19593
    “…Sequences of phenylpropanoid pathway genes available for common bean or other plant species were aligned, and the conserved regions were used to design sequence-specific primers. The PCR products were cloned and sequenced and the gene sequences along with common bean gene-based (g) markers were BLASTed against the Glycine max v.1.0 genome and the P. vulgaris v.1.0 (Andean) early release genome. …”
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    In silico comparison of genomic regions containing genes coding for enzymes and transcription factors for the phenylpropanoid pathway in Phaseolus vulgaris L. and Glycine max L. Merr
  14. 19594
    “…Rickettsia positive samples were further characterized using primers targeting three intergenic spacers (dksA-xerC, rmpE- tRNA(fMet) and mppA-purC). …”
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    Multispacer typing of Rickettsia isolates from humans and ticks in Tunisia revealing new genotypes
  15. 19595
    “…MMSR assay contained all the required reagents for qPCR including primers and probes, requiring only the addition of water and sample to perform qPCR. …”
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    Sample-ready multiplex qPCR assay for detection of malaria
  16. 19596
    “…RESULTS: Long-range amplification of full-length HLA genes from four loci was performed in separate polymerase chain reactions (PCR) using primers and PCR conditions that were optimized to reduce co-amplification of other HLA loci. …”
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    High-throughput multiplex HLA genotyping by next-generation sequencing using multi-locus individual tagging
  17. 19597
    “…HCV genotypes were classified by HCV-specific primers methods. HCV RNA loads were determined by fluorescence quantitative PCR. …”
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    Analytical Assessment of Interleukin - 23 and -27 Cytokines in Healthy People and Patients With Hepatitis C Virus Infection (Genotypes 1 and 3a)
  18. 19598
    “…Genotyping for the cytochrome P450 variant alleles was performed using predesigned primers. Amplification was done by PCR while differentiation between alleles was done by restriction fragment length polymorphism (PCR-RFLP) (for CYP2C8*2, CYP2C8*3) and sequencing (for CYP2B6*6, CYP3A5*3 and CYP3A4*1B). …”
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    Cytochrome P450 single nucleotide polymorphisms in an indigenous Tanzanian population: a concern about the metabolism of artemisinin-based combinations
  19. 19599
    “…The HIV-1 pol gene was amplified using primers for HIV-1 PR and RT and sequenced using the BigDye chemistry. …”
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    HIV-1 drug resistance-associated mutations among HIV-1 infected drug-naïve antenatal clinic attendees in rural Kenya
  20. 19600
    “…All 21 test strains except for S. maltophilia were negative for bla(L1), indicative of the high-specificity of the primers for the bla(L1). A total of 22 L1-positive isolates were identified for LAMP-based surveillance of bla(L1) from 105 ICU patients with clinically suspected multi-resistant infections. …”
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    Prevalence and detection of Stenotrophomonas maltophilia carrying metallo-β-lactamase blaL1 in Beijing, China
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