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19641por Del Giudice, Ilaria, Raponi, Sara, Della Starza, Irene, De Propris, Maria Stefania, Cavalli, Marzia, De Novi, Lucia Anna, Cappelli, Luca Vincenzo, Ilari, Caterina, Cafforio, Luciana, Guarini, Anna, Foà, Robin“…The standardized and most commonly applied methods to assess MRD in CLL are based on flow cytometry (FCM) and, to a lesser extent, real-time quantitative PCR (RQ-PCR) with allele-specific oligonucleotide (ASO) primers of immunoglobulin heavy chain genes (IgH). …”
Publicado 2019
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19642por Paguem, Archile, Abanda, Babette, Ndjonka, Dieudonné, Weber, Judith Sophie, Ngomtcho, Sen Claudine Henriette, Manchang, Kingsley Tanyi, Adoulmoumini, Mamoudou, Eisenbarth, Albert, Renz, Alfons, Kelm, Sørge, Achukwi, Mbunkah Daniel“…In the Sudano-Sahelian and Guinea Savannah of Cameroon the prevalence and genetic diversity of trypanosomes infecting cattle was investigated by microscopy of cattle blood buffy coat and molecular methods using generic primers targeting parts of the internal transcribed spacer 1 (ITS-1) and encoded glycosomal glyceraldehyde 3-phosphate dehydrogenase-gene (gGAPDH). …”
Publicado 2019
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19643por Nelsen, Donald J, Sinha, Rohita, Tyler, Aaron J, Westergaard, Jordyn, Nutt, Jamie, Wissel, Mark, Kleiboeker, Steve, Altrich, Michelle“…METHODS: Internal transcribed spacer (ITS) regions 1 and 2 were used for fungal identification. Primers were taken from published research and/or designed/modified by assessment in fungal sequence alignments. …”
Publicado 2019
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19644por Tyler, Aaron J, Grantham, James, Diaz, Manisha, Miralles, Mauricio, Bartlett, Katelyn, Foland, Derek, Berg, Amy, Howard, Karen, Nelsen, Donald J, Westergaard, Jordyn, Wissel, Mark, Kleiboeker, Steve, Altrich, Michelle“…METHODS: Flu A sequences (6,175) of the PA gene from the NCBI Influenza Virus Database collected over the last 5 years were compiled and aligned. Primers and probes were designed to target the I38 codon of the PA gene, and specific probes for each codon yielding a resistant amino acid mutation (I38T, -M, and -F) were designed. …”
Publicado 2019
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19645por Granger, Nicolas, Luján Feliu-Pascual, Alejandro, Spicer, Charlotte, Ricketts, Sally, Hitti, Rebekkah, Forman, Oliver, Hersheson, Joshua, Houlden, Henry“…SBF2 and its variant were sequenced using primers and PCRs. RNA was extracted from muscle of an unaffected and an affected dog and RT-PCR performed. …”
Publicado 2019
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19646por das Neves Costa, Larissa Cristina Prado, Teixeira, Dielle Monteiro, Portela, Ana Caroline Rodrigues, de Lima, Ian Carlos Gomes, da Silva Bandeira, Renato, Sousa Júnior, Edivaldo Costa, Siqueira, Jones Anderson Monteiro, Resque, Hugo Reis, da Silva, Luciana Damascena, Gabbay, Yvone Benchimol“…The GII.17-positive samples were retested by PCR using different sets of designed primers, which target a highly conserved capsid gene region. …”
Publicado 2019
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19647por Sholder, Gabriel, Lanz, Thomas A., Moccia, Robert, Quan, Jie, Aparicio-Prat, Estel, Stanton, Robert, Xi, Hualin S.“…RESULTS: Thorough optimization resulted in a protocol that takes less than 12 h to perform, does not require custom sequencing primers or instrumentation, and cuts over 90% of the costs associated with TruSeq, while still achieving accurate gene expression quantification (Pearson’s correlation coefficient with ERCC theoretical concentration r = 0.96) and differential gene detection (ROC analysis of 3’Pool-seq compared to TruSeq AUC = 0.921). …”
Publicado 2020
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19648“…The virus was identified as NDV by the HI test using specific anti-LaSota HI serum and RT-PCR with specific primers and probes. Propagation of the virus in the allantoic cavity of embryonated hen eggs produced a viral titer of 10(9.5) EID(50)/0.1 mL. …”
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19649por Megarani, Dorothea Vera, Nugroho, Herjuno Ari, Andarini, Zahrah Prawita, Surbakti, Yura Dwi Risa B. R., Widayanti, Rini“…Furthermore, polymerase chain reaction was done to amplify the mtDNA Cyt B using the CytBF and CytBR primers. Following sequencing, the analysis of genetic variation and the phylogenetic relationship was performed using MEGA version X software. …”
Publicado 2020
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19650“…The circular characteristic of circ_0058124 was identified by oligo (dT)(18) primers, Ribonuclease R (RNase R) and Actinomycin D (ActD), and its localization was determined by nuclear-cytoplasmic separation assay. …”
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19651por Raftery, Alexandra G., Jallow, Saloum, Coultous, Robert M., Rodgers, Jean, Sutton, David G. M.“…Blood samples underwent PCR analysis with specific Trypanosoma spp. primers and results were compared to the subject’s clinical and clinicopathological features. …”
Publicado 2020
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19652por Huang, Su-Hua, Su, Mei-Chi, Tien, Ni, Huang, Chien-Jhen, Lan, Yu-Ching, Lin, Chen-Sheng, Chen, Chao-Hsien, Lin, Cheng-Wen“…METHODS: Throat samples from 107 hospitalized patients with pneumonia and 175 outpatients with influenza-like illness were examined using real-time polymerase chain reaction assays with virus-specific primers, and then virus-positive specimens were confirmed by sequencing the polymerase chain reaction products. …”
Publicado 2017
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19653por Li, Xiaoyan, Kong, Mei, Su, Xu, Zou, Ming, Guo, Liru, Dong, Xiaochun, Li, Lin, Gu, Qing“…HAdV isolates were typed by hexon gene, fiber gene, and whole genome sequencing using primers designed in-house and compared to different type/serotype HAdVs downloaded from GenBank. …”
Publicado 2014
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19654“…In the present study, we aimed to develop a real-time RT-PCR method for detection of BVDV-1 in aerosol samples. METHODS: A pair of primers specific for highly conserved regions of the BVDV-1 5′-UTR was designed. …”
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19655por Freymuth, F., Vabret, A., Dina, J., Daubin, C., Gouarin, S., Petitjean, J., Charbonneau, P.“…Nucleic acid testing can subtype viruses using subtype-specific primers, and analyse strain variation through genetic. …”
Publicado 2007
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19656por Bojović, Katarina, Ignjatović, Ður -d ica, Soković Bajić, Svetlana, Vojnović Milutinović, Danijela, Tomić, Mirko, Golić, Nataša, Tolinački, Maja“…Although significant differences in SCFA levels were not detected between NDD patients and the Control group, a positive correlation was noted between number of rDNA amplicons obtained with universal primers and level of propionic acid, as well as a trend for levels of total SCFAs and butyric acid in the Control group. …”
Publicado 2020
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19657por Katongole, Paul, Nalubega, Fatuma, Florence, Najjuka Christine, Asiimwe, Benon, Andia, Irene“…The isolates were later subjected PCR for the detection of Urovirulence genes namely; Pap, Fim, Sfa, Afa, Hly and Cnf, using commercially designed primers. RESULTS: In this study, 62.5% (125/200) were positive biofilm formers and 78% (156/200) of these were multi-drug resistant (MDR). …”
Publicado 2020
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19658“…METHODS: We used the primers and single-quencher probes recommended by the CDC (N1, N2 and N3) in the USA and the NIID (N1 and N2) in Japan. …”
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19659por Abd El-Hamid, Hatem S., Shafi, Manal E., Albaqami, Najah M., Ellakany, Hany F., Abdelaziz, Naglaa M., Abdelaziz, Mohamed N., Abd El-Hack, Mohamed E., Taha, Ayman E., Alanazi, Khalid M., Elbestawy, Ahmed R.“…These samples were submitted to molecular examination using qRT-PCR specific primers for AOAV-1, highly pathogenic avian influenza (HPAI-H5), low pathogenic avian influenza (LPAI-H9) and infectious bronchitis virus (IBV). …”
Publicado 2020
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19660por Pinthong, Nattapon, Limudomporn, Paviga, Vasuvat, Jitlada, Adisakwattana, Poom, Rattaprasert, Pongruj, Chavalitshewinkoon-Petmitr, Porntip“…From bio-informatics analysis, primers were designed for amplification, insertion into pBAD202/D-TOPO and heterologous expression in Escherichia coli of recombinant PfMAG. …”
Publicado 2020
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