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  1. 19801
    “…DNA extracted from stool was used to sequence the 16S rRNA gene by Illumina MiSeq using universal primers. Resulting operational taxonomic unit tables were analyzed for differences between years and hospitals using linear discriminant analysis effect size algorithm (LEfSe; significance, p < 0.05). …”
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    Intestinal microbiota of preterm infants differ over time and between hospitals
  2. 19802
    “…RESULTS: The qPCR performed on CS samples showed better sensitivity (87%) and specificity (96%) than assays carried out using BC samples, regardless of the primers used. The haematochemical parameters haemoglobin and globulins were found to be significantly associated with qPCR positivity. …”
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    Application of qPCR in conjunctival swab samples for the evaluation of canine leishmaniasis in borderline cases or disease relapse and correlation with clinical parameters
  3. 19803
    “…METHOD: Endocervical swabs, collected from 200 infertile women were tested for C. trachomatis by three different PCR assays viz. in-house real time-PCR targeting the cryptic plasmid using published primers, along with omp1 gene and cryptic plasmid based conventional PCR assays. …”
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    Diagnostic efficacy of a real time-PCR assay for Chlamydia trachomatis infection in infertile women in north India
  4. 19804
    “…Rotavirus G and P genotypes were determined by reverse transcription polymerase chain reaction (RT-PCR) using consensus primers detecting the VP7 and VP4 genes, followed by semi-nested type-specific multiplex PCR. …”
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    Prevalence of Rotavirus Genotypes in Children Younger than 5 Years of Age before the Introduction of a Universal Rotavirus Vaccination Program: Report of Rotavirus Surveillance in Turkey
  5. 19805
    “…Since blackberries are tetraploids, the possibility of artefactual gene chimeras resulting from mis-assembly was tested with one of the genes sequenced by RNAseq, Chalcone Synthase (CHS). cDNAs encoding this protein were cloned and sequenced. Primers designed to the assembled sequences accurately distinguished different contigs, at least for chalcone synthase genes. …”
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    RNA-Seq analysis and transcriptome assembly for blackberry (Rubus sp. Var. Lochness) fruit
  6. 19806
    “…The screening of the ctx genes (ctxA2-B gene) were performed by PCR method using a pair of primers; C2F (5′-AGGTGTAAAATTCCTTGACGA-3′) and C2R (5′-TCCTCAGGGTATCCTTCATC-3′) to identify the toxigenic strains of V. cholerae. …”
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    Multidrug resistant Vibrio cholerae O1 from clinical and environmental samples in Kathmandu city
  7. 19807
    “…METHODS: Six pairs of primers were designed based on cytochrome b gene sequences available in GenBank of the following potential hosts: dog, cat, horse, chicken, black rat, and human. …”
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    Identification of phlebotomine sand fly blood meals by real-time PCR
  8. 19808
    “…To demonstrate transfection efficiency of the constructed carrier, reverse transcription polymerase chain reaction (RT-PCR) was carried out using human insulin specific primers on total RNA extracted from upper part of small intestine of 48-hour post-transfected rats (sampled by simple random selection, n = 3). …”
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    Evaluation of In Vivo Transfection Efficiency of Eudragit Coated Nanoparticles of Chitosan-DNA: A pH-sensitive System Prepared for Oral DNA Delivery
  9. 19809
    “…A total of 15 SCoT and 17 CBDP primers detected genetic polymorphism among 39 Jojoba genotypes (22 females and 17 males). …”
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    Genetic diversity analysis among male and female Jojoba genotypes employing gene targeted molecular markers, start codon targeted (SCoT) polymorphism and CAAT box-derived polymorphism (CBDP) markers
  10. 19810
    “…Using the chiA-targeted PCR, which is based on degenerate primers, the five fosmids all produced amplicons, of which the sequences were related to predicted chitinolytic enzyme-encoding genes of four different host organisms, including Stenotrophomonas maltophilia. …”
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    A novel salt-tolerant chitobiosidase discovered by genetic screening of a metagenomic library derived from chitin-amended agricultural soil
  11. 19811
    “…In addition, murine Lin-/Sca-1+ cells were cultured with CFSE-labelled LDEV isolated from rats, and RT-PCR analysis was performed on LDEV+ and – cells using species-specific primers for surfactant (rat/mouse hybrid co-cultures). …”
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    Lung-derived exosome uptake into and epigenetic modulation of marrow progenitor/stem and differentiated cells
  12. 19812
    “…METHODS: Our strategy, named normalized-BSP (NBSP), takes advantage of tailed C-balanced primers and a normalization procedure based on C/T ratio to overcome BSP-associated noise problems and nucleotide signal unbalance. …”
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    An improved sequencing-based strategy to estimate locus-specific DNA methylation
  13. 19813
    “…METHODS: 30 pairs of primers were designed based on the full genome sequence of B. orientalis (unpublished data) and by aligning reported apicoplast genomes of Babesia bovis and Theileria parva. …”
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    Characterization and annotation of Babesia orientalis apicoplast genome
  14. 19814
    por Szczecińska, Monika, Sawicki, Jakub
    Publicado 2015
    “…The results will supply valuable information about genetic variation, which can be used in the process of designing primers for population studies and conservation genetics research. …”
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    Genomic Resources of Three Pulsatilla Species Reveal Evolutionary Hotspots, Species-Specific Sites and Variable Plastid Structure in the Family Ranunculaceae
  15. 19815
    “…Suspected colonies of B. pseudomallei were identified by biochemical and serological test, and by polymerase chain reaction (PCR) using 16s rRNA specific primers. Blood samples were collected from 940 healthy individuals of four districts to determine anti- B. pseudomallei IgG antibody levels by indirect enzyme linked immunosorbent assay (ELISA) using sonicated crude antigen. …”
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    Burkholderia pseudomallei: Its Detection in Soil and Seroprevalence in Bangladesh
  16. 19816
    “…RESULTS: PCR from mouse retinal cDNA using Bbs5-specific primers amplified a unique cDNA that was shown to be a splice variant of BBS5 resulting from the use of cryptic splicing sites in Intron 7. …”
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    A Splice Variant of Bardet-Biedl Syndrome 5 (BBS5) Protein that Is Selectively Expressed in Retina
  17. 19817
    “…The manually identified Fusarium spp. were further confirmed by PCR-based detection using ITS1 and ITS4 primers followed by sequencing of the PCR amplicons. …”
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    Diversity of toxic and phytopathogenic Fusarium species occurring on cereals grown in Karnataka state, India
  18. 19818
    por Vojvodić, S, Ademović-Sazdanić, D
    Publicado 2015
    “…Killer cell immunoglobulin-like receptor low resolution genotyping was performed by the polymerase chain reaction (PCR)-sequence-specific primers (SSP)/sequence-specific oligonucleotide (SSO) method and haplotype assignment was done by gene content analysis. …”
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    KIR and HLA haplotype analysis in a family lacking the KIR 2DL1-2DP1 genes
  19. 19819
    “…Does this parasite develop with equal efficiency in both snail species, and does this have implications for transmission in different habitat types? METHODS: Primers for PCR amplification of the S. mansoni ND5 gene were designed and tested for sensitivity and specificity. …”
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    Relative compatibility of Schistosoma mansoni with Biomphalaria sudanica and B. pfeifferi from Kenya as assessed by PCR amplification of the S. mansoni ND5 gene in conjunction with traditional methods
  20. 19820
    “…The target for the special set of six LAMP primers used was the VP(AHPND) Pir(vp)A gene. The LAMP reaction was carried out at 65°C for 45 min followed by addition of the red AuNP solution and further incubation at 65°C for 5 min, allowing any Pir(vp)A gene amplicons present to hybridize with the probe. …”
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    Sensitive Visual Detection of AHPND Bacteria Using Loop-Mediated Isothermal Amplification Combined with DNA-Functionalized Gold Nanoparticles as Probes
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