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  1. 19861
    “…The SMN1 and SMN2 were simultaneously amplified by polymerase chain reaction using the same primers. Mutation Surveyor software was used to detect and quantify the SMN1 variants by calculating allelic proportions in Sanger sequencing. …”
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    Diagnosis of Spinal Muscular Atrophy: A Simple Method for Quantifying the Relative Amount of Survival Motor Neuron Gene 1/2 Using Sanger DNA Sequencing
  2. 19862
    “…RESULTS: Six degenerate primers based on the conserved domains of protein kinases were used in a PK-profiling assay including cDNA from mock-inoculated leaves and subtracted cDNA. …”
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    PK-profiling method for identifying the expression of resistance-associated genes in partially resistant oats to crown rust
  3. 19863
    “…All mosquito species were collected in dry tropical forest of the Caribbean area. Universal primers for NS5 gene (958 pb), RT-PCR for flavivirus and sequencing were used for molecular identification of viruses detected. …”
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    First report of Culex flavivirus infection from Culex coronator (Diptera: Culicidae), Colombia
  4. 19864
    “…Both species have also incorporated novel DNA in the LSC region adjacent to the LSC/IRa junction, and BLAST searches revealed it had a 100 bp match (86% sequence identity) to noncoding mitochondrial DNA. Prangos‐specific primers were developed for the variable accD/psaI intergenic spacer and preliminary PCR‐surveys suggest that this region will be useful for future phylogeographic and population‐level studies.…”
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    A comparative analysis of complete plastid genomes from Prangos fedtschenkoi and Prangos lipskyi (Apiaceae)
  5. 19865
    “…Telomere lengths were assessed by quantitative real-time PCR (qRT-PCR) using telomere specific primers and DNA extracted from the cells, which was further adjusted with single-copy gene and reference DNA (ddC(t)). …”
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    Walnut phenolic extracts reduce telomere length and telomerase activity in a colon cancer stem cell model
  6. 19866
    “…Genetic study was executed by polymerase chain reaction sequence-specific primers and sequence-based typing method. Statistical analysis was performed by using the “Statgraphics software systems”. …”
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    Redox, immune and genetic biomarker system for personalized treatments in colorectal cancer
  7. 19867
    “…L. asiaticus genome. Sequence specific primers were designed for reverse transcription (RT) and quantitative PCR (qPCR), and expression was validated for 20 of the effector candidates in infected citrus with multiple genetic background. …”
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    Temporal and spatial detection of Candidatus Liberibacter asiaticus putative effector transcripts during interaction with Huanglongbing-susceptible, −tolerant, and -resistant citrus hosts
  8. 19868
    “…Six IB live vaccines were used as control, the reference vaccines represent IBV strains including H120, H52, 4/91, CR88, 233A, and 1-96. Primers XCe2+ and XCe2- were used to amplify S1 gene partially. …”
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    Genetic characterization of S1 gene of infectious bronchitis virus isolated from commercial poultry flocks in West Java, Indonesia
  9. 19869
    “…The causable isolates were identified by the VITEK 2 Compact system, matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and molecular identification methods using PCR with universal and species-specific primers. Antimicrobial susceptibility testing was performed using the broth microdilution method. …”
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    Nosocomial bloodstream infection and the emerging carbapenem-resistant pathogen Ralstonia insidiosa
  10. 19870
    “…In the resistant strains, ERG11 genes were amplified by specific primers. Subsequently, cycle sequencing reactions were performed on purified polymerase chain reaction (PCR) products in forward and reverse directions. …”
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    Detection of ERG11 point mutations in Iranian fluconazole-resistant Candida albicans isolates
  11. 19871
    “…To determine the virus genome sequence, high throughput sequencing (HTS) was performed. RT-PCR primers were designed to confirm virus infection and to be used as a diagnostic tool. …”
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    Characterisation of a novel nucleorhabdovirus infecting alfalfa (Medicago sativa)
  12. 19872
    “…We present specific and reproducible diagnostic assays based on conventional PCR targeting SIX genes, using as templates DNA extracted from pure Foc cultures. Sets of primers specifically amplify regions of: SIX6 in Foc race 1, SIX1 gene in TR4, SIX8 in subtropical race 4, SIX9/SIX10 in Foc VCG 0121, and SIX13 in Foc VCG 0122. …”
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    Molecular Diagnostics of Banana Fusarium Wilt Targeting Secreted-in-Xylem Genes
  13. 19873
    “…Typing of HLA was carried out by polymerase chain reaction (PCR) amplification with sequence-specific primers (SSP) method. SPSS software was used for the statistical analyses. …”
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    Association of human leukocyte antigen-DRB haplotype in multiple sclerosis population of Khuzestan, Iran
  14. 19874
    “…Trichothecene genotype prediction was carried out by primers 3CON/3NA/3D15A/3D3 or Tri13F/Tri13R, Tri303F/Tri303R and Tri315F/Tri315R. …”
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    Monitoring of Fusarium Species and Trichothecene Genotypes Associated with Fusarium Head Blight on Wheat in Hebei Province, China
  15. 19875
    “…The mice tails DNA were genotyped using the primers for LoxP sites and Cre recombinase, respectively. …”
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    Generation and Validation of miR-100 Hepatocyte-Specific Knock-Out Mice
  16. 19876
    “…HPV DNA was isolated and amplified with PCR, with modified and/or original GP5(+)/GP6(+) primers. Samples were genotyped with a gene array assay and/or population sequencing. …”
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    Low concordance of oral and genital HPV infection among male patients with sexually transmitted infections in Vietnam
  17. 19877
    “…RESULTS: The LF-RPA assay successfully amplified P. beihaiensis DNA using a set of primers of 20–25 bp in length. After incubation at 37 °C for 25 min, results were read within 5 min by the naked eye on a lateral flow strip. …”
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    Utilization of recombinase polymerase amplification combined with a lateral flow strip for detection of Perkinsus beihaiensis in the oyster Crassostrea hongkongensis
  18. 19878
    “…Metabarcoding was performed on the samples using generic COI primers. Molecular operational taxonomic unit (MOTUs) were used to assess dissimilarities between spatial and temporal replicates. …”
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    The effects of spatial and temporal replicate sampling on eDNA metabarcoding
  19. 19879
    “…DNA extraction, nested PCR using mitochondrial (cox3 gene) and ribosomal (18S rDNA) primers, electrophoresis and sequencing were conducted in order to identify Plasmodium spp. from the samples. …”
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    Prevalence of Plasmodium parasites in non-human primates and mosquitoes in areas with different degrees of fragmentation in Colombia
  20. 19880
    “…Also, through whole chloroplast genome comparisons and DNA polymorphism tests, ten divergent hotspots were identified. Potential primers were designed creating genomic tools to further molecular studies within the tribe. …”
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    Comparative genomics of 11 complete chloroplast genomes of Senecioneae (Asteraceae) species: DNA barcodes and phylogenetics
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