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  1. 19881
    “…DNA was extracted, amplified using primers targeting the V3-V4 variable region of the 16S rRNA-gene, and then sequenced and processed through a hybrid Qiime MICCA bioinformatics pipeline. …”
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    2576. The Microbiome of Recurrent Bacterial Vaginosis Compared with Asymptomatic Controls
  2. 19882
    “…The transgenic lines were validated by PCR analysis using primers specific to the nptII gene. To obtain uniformly transformed plantlets, chimerism was diluted by subculturing and regenerating the transgenic shoots on a selective medium containing kanamycin (150 mg/l) for five cycles. …”
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    A simple and rapid protocol for the genetic transformation of Ensete ventricosum
  3. 19883
    “…Two different mutational analysis assays of a representative tumor area were performed: first, a singleplex PCR assay for evaluation of the TERT promoter region (TERTSeqS) and second, a multiplex PCR assay using primers designed to amplify regions of interest of 10 (FGFR3, PIK3CA, TP53, HRAS, KRAS, ERBB2, CDKN2A, MET, MLL, and VHL) genes (UroSeqS). …”
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    Incidence and Distribution of UroSEEK Gene Panel in a Multi-institutional Cohort of Bladder Urothelial Carcinoma
  4. 19884
    “…RESULTS: In the present study, five loci containing null alleles were excluded and 15 pairs of SSR (simple sequence repeat) primers were used to analyze the genetic diversity and population structure of 384 individuals from six provenances (Missouri, Iowa, Washington, Louisiana, and Tennessee (USA), and Quebec in Canada) of P. deltoides. …”
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    Genetic diversity and population structure of black cottonwood (Populus deltoides) revealed using simple sequence repeat markers
  5. 19885
    “…Polymerase chain reaction was performed using oligonucleotide primers targeting the entire open reading frame of G protein-coupled receptors (GPCR) gene and gene sequences were analyzed. …”
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    Capripoxviruses: Exploring the genetic relatedness between field and vaccine strains from Egypt
  6. 19886
    “…Two HIV-1 genes; protease and reverse transcriptase, were amplified, sequenced using gene-specific primers and analyzed for subtype and drug resistance mutations using the Stanford HIV Database. …”
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    High resistance to reverse transcriptase inhibitors among persons infected with human immunodeficiency virus type 1 subtype circulating recombinant form 02_AG in Ghana and on antiretroviral therapy
  7. 19887
    “…RT-PCR amplification with primers specific to individual mycoviruses revealed that mitoviruses were the most prevalent and the alphavirus-like virus and chrysovirus were the least prevalent. …”
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    Virome Identification and Characterization of Fusarium sacchari and F. andiyazi: Causative Agents of Pokkah Boeng Disease in Sugarcane
  8. 19888
    “…DNA extracted from spiked sputa was subjected to (i) real-time PCR for Aspergillus DNA detection and (ii) combined metagenomic analyses targeting barcoded primers for fungal ITS1 and ITS2, and bacterial V1-V2 and V3-V4 16S regions. …”
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    Combined bacterial and fungal targeted amplicon sequencing of respiratory samples: Does the DNA extraction method matter?
  9. 19889
    “…Therefore, identifying chitinases with higher enzymatic activity and tolerance is of great importance. METHODS: Primers were designed using the genomic database of Paenibacillus chitinolyticus NBRC 15660. …”
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    Cloning, expression and characterization of a chitinase from Paenibacillus chitinolyticus strain UMBR 0002
  10. 19890
    “…Samples were screened for adenovirus DNA using degenerate primers and polymerase chain reaction. Adenovirus sequences were detected in eighty-three samples representing thirty-five novel amino acid sequences. …”
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    Opportunistic sampling of wild native and invasive birds reveals a rich diversity of adenoviruses in Australia
  11. 19891
    “…Following a tissue diagnosis of ALCL, ALK+, RNA from the biopsy was extracted from Formalin-fixed paraffin-embedded (FFPE) tissue and prepared for Anchored Multiplex PCR using the Archer® FusionPlex® v2 assay, which employs unidirectional gene-specific primers using NGS to detect novel or unknown gene partners. …”
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    Diagnostic Capability of Next-Generation Sequencing Fusion Analysis in Identifying a Rare CASE of TRAF1-ALK-Associated Anaplastic Large Cell Lymphoma
  12. 19892
    “…The genotypes were detected using polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) methods with specific primers and restrictase for each gene. The statistical analysis discovered significant associations (p < 0.05) between the TGFβ3 SNP and the following parameters: BW at 21, 28 and 35 days, trunk weight and slaughter value. …”
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    Associations Between IGF1, IGFBP2 and TGFß3 Genes Polymorphisms and Growth Performance of Broiler Chicken Lines
  13. 19893
    por Punja, Zamir K., Holmes, Janesse E.
    Publicado 2020
    “…Seeds produced on hermaphroditic plants and seeds derived from cross-fertilization were germinated and seedlings were compared for gender ratios using a PCR-based assay as well as for the extent of genetic variation using six ISSR primers. Nei’s index of gene diversity and Shannon’s Information index were compared for these two populations. …”
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    Hermaphroditism in Marijuana (Cannabis sativa L.) Inflorescences – Impact on Floral Morphology, Seed Formation, Progeny Sex Ratios, and Genetic Variation
  14. 19894
    “…DNA was extracted and in-house nested PCR using primers specific for both SPG and TG pathogens was used. …”
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    Rickettsial Diseases: Not Uncommon Causes of Acute Febrile Illness in India
  15. 19895
    “…There were three trials of 18 Fragment Length Polymorphism (AFLP) EcoRI/MseI primers to identify the presence of the 5E(b) chromosome in 105 ISTG plants, along with four wheat addition lines and substitution lines for the 5E(b) chromosome. …”
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    Production and First Assessment of Iranian Secondary Tritipyrum Genotypes by GISH and AFLP Markers
  16. 19896
    “…To this end, we used 29-mers of RNA (as template) containing the modifications at position-18 and reverse transcribed DNA using 17-mers, 18-mers, or 19-mers (as primers). Generally reactivity of the viral RTs, AMV / HIV / MMLV, towards cDNA synthesis was similar for templates containing G or I as well as for those with 8-oxoG or 8-oxoI. …”
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    Translesion synthesis by AMV, HIV, and MMLVreverse transcriptases using RNA templates containing inosine, guanosine, and their 8-oxo-7,8-dihydropurine derivatives
  17. 19897
    “…Methods: To discriminate ctDNA mutations from wild-type cfDNA, allele-specific primers were designed for the amplification of three clinically important DNA point mutations in CRC including KRAS G12V, KRAS G13D and BRAF V600E. …”
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    Multiplex detection of ctDNA mutations in plasma of colorectal cancer patients by PCR/SERS assay
  18. 19898
    “…Genotyping was performed by PCR–sequence specific primers, and the MBL serum concentrations were evaluated by ELISA. …”
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    Association of MBL2 Exon 1 Polymorphisms With Multibacillary Leprosy
  19. 19899
    “…However, probably due to mismatching of the nanopore sequence primers, the nanopore sequencing platform identified Corynebacterium in much lower abundance compared to Illumina sequencing. …”
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    Comparison of Illumina versus Nanopore 16S rRNA Gene Sequencing of the Human Nasal Microbiota
  20. 19900
    “…IFA-positive pools were tested by qRT-PCR with genus-specific primers targeting the flavivirus NS5 gene to confirm IFA-positive results and sequenced to identify the species. …”
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    Detection of Zika and dengue viruses in wild-caught mosquitoes collected during field surveillance in an environmental protection area in São Paulo, Brazil
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