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  1. 19921
    “…Mismatches to known 16S rRNA gene primers were identified among 16S rRNA genes of DPANN archaea. …”
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    Comparative Genomics Provides Insights into the Genetic Diversity and Evolution of the DPANN Superphylum
  2. 19922
    “…The genomic DNA was isolated and used in polymerase chain reaction (PCR) with four sets of specific primers. The PCR-amplified DNA fragments were sequenced and analyzed for the NOD2 mutations. …”
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    Detection of mutations in NOD2/CARD15 gene in Arab patients with Crohn's disease
  3. 19923
    “…A total of 66 ISSR bands were generated from A. villosum populations using the selected six ISSR primers; 56 bands, 84.85% for all the seven A. villosum populations were polymorphic. …”
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    DNA barcode reference library construction and genetic diversity and structure analysis of Amomum villosum Lour. (Zingiberaceae) populations in Guangdong Province
  4. 19924
    “…In the present study, we investigated by PCR, using consensus primers, the distribution of hmqABCDEFG in a collection of 312 Bcc strains (222 of clinical and 90 of environmental origins) belonging to 18 Bcc species. …”
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    Presence of the Hmq System and Production of 4-Hydroxy-3-Methyl-2-Alkylquinolines Are Heterogeneously Distributed between Burkholderia cepacia Complex Species and More Prevalent among Environmental than Clinical Isolates
  5. 19925
    “…Polymerase chain reaction (PCR) was performed through the detection of the 16S rRNA gene, with multiple sets of primers for the detection of Mycobacterium tuberculosis complex (MTBC) and Mycobacterium avium complex (MAC). …”
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    Mycobacterium Tuberculosis and Avium Complex Investigation among Malaysian Free-Ranging Wild Boar and Wild Macaques at Wildlife-Livestock-Human Interface
  6. 19926
    “…We aimed to characterize the CRISPR-Cas system in the pathogenic Gram-positive bacterium Staphylococcus lugdunensis and determine its association with sequence types (STs) determined by multilocus sequence typing (MLST) and oxacillin susceptibility. Primers were designed to detect and sequence types IIIA and IIC CRISPR-Cas in 199 S. lugdunensis isolates. …”
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    Phylogenetic Distribution of CRISPR-Cas Systems in Staphylococcus lugdunensis
  7. 19927
    “…A combination of PCR with degenerate primers based on BAHD hydroxycinnamoyl-CoA transferase sequences and 5′ and 3′ rapid amplification of cDNA ends was used to clone two nearly identical cDNAs from red clover. …”
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    Red Clover HDT, a BAHD Hydroxycinnamoyl-Coenzyme A:L-3,4-Dihydroxyphenylalanine (L-DOPA) Hydroxycinnamoyl Transferase That Synthesizes Clovamide and Other N-Hydroxycinnamoyl-Aromatic Amino Acid Amides
  8. 19928
    “…The presence of the gene was postulated by genotyping with specif ic primers (VENTRIUP-LN2) and phytopathological tests with avirulent clones of the fungus. …”
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    The gene Sr38 for bread wheat breeding in Western Siberia
  9. 19929
    “…In samples with low bacterial load, human mitochondrial sequences were highly amplified using commonly used, bacterial-targeted 16S rRNA primers. Only half of the meconium samples contained sufficient bacterial material to study the microbiome using a standard approach. …”
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    Meconium Microbiome of Very Preterm Infants across Germany
  10. 19930
    “…An in silico analysis was performed and primers were designed from a region where sequences were dissimilar with other races to develop a specific marker for Foc R1, R4, TR4, and STR4. …”
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    Development of PCR-Based Race-Specific Markers for Differentiation of Indian Fusarium oxysporum f. sp. cubense, the Causal Agent of Fusarium Wilt in Banana
  11. 19931
    “…MATERIALS AND METHODS: After DNA isolation from 56 positive samples, the LCR of HPV 16 and HPV 18 were amplified using specific primers, and phylogenetic trees were drawn through MEGA X. …”
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    Genetic Diversity of HPV 16 and HPV 18 Based on Partial Long Control Region in Iranian Women
  12. 19932
    “…However, it is still unclear whether this tendency to conform to the partner’s behaviour can be influenced by social stimuli and/or the neurohormone oxytocin as primers to prosocial predispositions. In Experiment I, eighty two dogs were tested using Prato-Previde et al.’s food quantity preference task. …”
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    Social Stimulation by the Owner Increases Dogs’ (Canis familiaris) Social Susceptibility in a Food Choice Task—The Possible Effect of Endogenous Oxytocin Release
  13. 19933
    “…Stool was banked frozen until processed for sequencing using 16S Ribosomal primers. RESULTS: Three F2 litters were comprised of ratios of HZ:WT:SUCNR1-KO of 14:7:2. …”
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    A183 SUCCINATE IN THE RELATIONSHIP BETWEEN INFLAMMATORY BOWEL DISEASE AND OBESITY IN SUCCINATE RECEPTOR DEFICIENT MICE
  14. 19934
    “…Partial sequence analysis of ITS5-5.8-ITS4 region of the nuclear ribosomal DNA with universal primers identified YD5, YE1, and YSW1 as Saccharomyces cerevisiae (S. cerevisiae), Zygosaccharomyces bailii (Z. bailii), and Saccharomyces kudriavzevii (S. kudriavzevii), respectively. …”
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    Tobacco Plant Growth-Promoting and Antifungal Activities of Three Endophytic Yeast Strains
  15. 19935
    “…The presence of six most common gene mutations, namely, Intron 2 (c.293-13A/C>G), c.844G>T (p.Val282Leu), c.1019G>A (p.Arg340His), c.92C>T (p.Pro31Leu), c.955C>T (p.Gln319*), and c.518T>A (p.Ile173Asn) in CYP21A2 gene were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using specific primers. RESULTS: Out of 16 classic CAH females analyzed, salt-wasting (SW) form was present in 12 (75%) and simple virilizing form in four (25%) children. …”
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    The Spectrum of CYP21A2 Gene Mutations from 16 Families of Congenital Adrenal Hyperplasia: Genotype-Phenotype Correlation
  16. 19936
    “…Polymerase chain reaction (PCR) was used to confirm the identity of the isolates via specific primers and further used to assay the diarrheagenic determinants of the E. coli isolates. …”
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    Processed ready-to-eat (RTE) foods sold in Yenagoa Nigeria were colonized by diarrheagenic Escherichia coli which constitute a probable hazard to human health
  17. 19937
    “…Quantitative rRT-PCR with primers and probes targeting gene N1 were used for SARS-CoV-2 RNA quantification. …”
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    Monitoring SARS-CoV-2 in air and on surfaces and estimating infection risk in buildings and buses on a university campus
  18. 19938
    “…CD274 mRNA expression was identified using three independent sets of primers and TaqMan® probes targeting exon 1–2, exon 3–4, and exon 5–6. …”
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    Expression of CD274 mRNA Measured by qRT-PCR Correlates With PD-L1 Immunohistochemistry in Gastric and Urothelial Carcinoma
  19. 19939
    “…We have designed three pairs of oligos (a.k.a primers or oligonucleotides) to identify R. equi and MLS(B) (macrolide, lincosamide, streptogramin B) resistance genes erm(46) and erm(51) that can be used in single-plex and multiplex qPCR assays. …”
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    Novel Quantitative PCR for Rhodococcus equi and Macrolide Resistance Detection in Equine Respiratory Samples
  20. 19940
    “…The quantitative PCR (qPCR) primers were designed for PS marker genes glucose dehydrogenase (gcd), pyrroloquinoline quinone biosynthesis protein C (pqqC), and phosphatase (pho). …”
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    Designing Synergistic Biostimulants Formulation Containing Autochthonous Phosphate-Solubilizing Bacteria for Sustainable Wheat Production
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