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15221por Mukherjee, Prabuddha, Aksamitiene, Edita, Alex, Aneesh, Shi, Jindou, Bera, Kajari, Zhang, Chi, Spillman, Darold R., Marjanovic, Marina, Fazio, Michael, Seth, Punit P., Frazier, Kendall, Hood, Steve R., Boppart, Stephen A.“…The CARS modality captured the dynamic lipid distributions and overall morphology of the cells; two-photon fluorescence (2PF) measured the time- and dose-dependent localization of ASOs delivered by a modified treatment of suspension cells. Our results show that in macrophages, the uptake rate of PS-ASOs did not significantly differ from that of GalNAc-PS-ASOs. …”
Publicado 2022
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15222por El-Kassas, Seham, Aljahdali, Nesreen, Abdo, Safaa E., Alaryani, Fatima S., Moustafa, Eman M., Mohamed, Radi, Abosheashaa, Wesam, Abdulraouf, Esraa, Helal, Mohamed Atef, Shafi, Manal E., El-Saadony, Mohamed T., El-Naggar, Karima, Conte-Junior, Carlos Adam“…Then, each fish in the first two replicates was injected with 0.2 mL of PBS, while the others were injected with 0.2 mL of A. hydrophila suspension (1.8 × 10(6) CFU/mL). Healthy fish fed on M. oleifera leaf powder showed enhanced immune response manifested by significant increases in phagocytic and lysozyme activities with a marked H/L ratio (P < 0.05). …”
Publicado 2022
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15223por Takahashi, Kazuya, Kurashina, Kentaro, Saito, Shin, Kanamaru, Rihito, Ohzawa, Hideyuki, Yamaguchi, Hironori, Miyato, Hideyo, Hosoya, Yoshinori, Lefor, Alan Kawarai, Sata, Naohiro, Kitayama, Joji“…METHODS: A total of 143 single cell suspensions derived from ascites or peritoneal lavages from patients with advanced gastric cancer (GC) were stained with monoclonal antibodies to CD45 and to CD326 as well as 4,6‐diamidino‐2‐phenylindole (DAPI) and FVS780. …”
Publicado 2020
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15224por Zhang, Maolin, Niibe, Kunimichi, Kondo, Takeru, Limraksasin, Phoonsuk, Okawa, Hiroko, Miao, Xinchao, Kamano, Yuya, Yamada, Masahiro, Jiang, Xinquan, Egusa, Hiroshi“…Moreover, we developed an efficient and simple approach for directly guiding iPSC-Tet/BMP-4 differentiation into chondrocytes in scaffold-free cartilaginous pellets using a combination of transcriptional activation of BMP-4 and a 3D shaking suspension culture system. In chondrogenic induction medium, shaking culture alone significantly upregulated the chondrogenic markers Sox9, Col2a1, and Aggrecan in iPSCs-Tet/BMP-4 by day 21. …”
Publicado 2022
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15225por Alsafar, Habiba, Albreiki, Mohammed, Mousa, Mira, Azman, Syafiq Kamarul, Vurivi, Hema, Waasia, Fathimathuz, Ruta, Dymitr, Alhosani, Farida, Almazrouei, Shereena, Abuyadek, Rowan, Selvaraj, Francis, Chaves-Coira, Irene, Zvereff, Val, Abdel-Malek, Mohamed A. Y., Alkaabi, Nawal, Uddin, Maimunah, Al Awadhi, Tayba, Al Marzouqi, Nada, Al Attar, Fatma, Al Shamsi, Safeiya, Al Shehhi, Fatima, Alteneiji, Hala, Mohamed, Kalthoom, Al Muhairi, Noor, AlRand, Hussain, Fikri, Asma, Henschel, Andreas“…As a result of these measures, flight suspension, nationwide RT-PCR and surveillance of viral sequences were extensively implemented. …”
Publicado 2022
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15226“…To create glucose gradients across the barrier, uninfected erythrocyte or IE suspension with a higher glucose concentration was introduced into the “trophoblast side” and a culture medium with lower glucose concentration was introduced into the “endothelial side”. …”
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15227por Jiang, Wenting, Jiang, Dawei, Li, Lu, Wan, Bo, Wang, Jiabin, Wang, Panpan, Shi, Xuejian, Zhao, Qi, Song, Jinxing, Zhu, Zixiang, Ji, Pengchao, Zhang, Gaiping“…We integrated the CD2v protein extracellular domain sequence (CD2v-Ex, 1–588 bp) of the highly pathogenic strain China/2018/AnhuiXCGQ into the genome of suspension culture-adapted Chinese hamster Ovary-S (CHO-S) cells using lentivirus vectors (LVs). …”
Publicado 2022
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15228por Yamanishi, Kyosuke, Doe, Nobutaka, Mukai, Keiichiro, Hashimoto, Takuya, Gamachi, Naomi, Hata, Masaki, Watanabe, Yuko, Yamanishi, Chiaki, Yagi, Hideshi, Okamura, Haruki, Matsunaga, Hisato“…In Il18(−/−) mice exposed to acute stress, the immobility times in both the forced swim test and tail suspension test were decreased, although no difference was observed in Il18(+/+) mice. …”
Publicado 2022
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15229“…This suggested that suspension-size particles are the main component of the soil GSD and decrease with the increasing depth in the profiles at the Tamarix cones. …”
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15230por Hergueta‐Castillo, María Elena, López‐Ruiz, Rosalía, Garrido Frenich, Antonia, Romero‐González, Roberto“…RESULTS: A research technique based on ultra‐high‐performance liquid chromatography (UHPLC) coupled to a Quadrupole‐Orbitrap mass analyzer was successfully applied to characterize the composition of six different PPPs in terms of the presence of co‐formulants and types of formulations: emulsifiable concentrate (EC), emulsion in water (EW), suspension concentrate and water‐dispersible granule. …”
Publicado 2022
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15231por Murray-Brown, William, Guo, Yanxia, Small, Annabelle, Lowe, Katie, Weedon, Helen, Smith, Malcolm D, Lester, Susan E, Proudman, Susanna M, Rao, Navin L, Hao, Ling-Yang, Nagpal, Sunil, Wechalekar, Mihir D“…METHODS: Fresh dissociated tissue and peripheral blood mononuclear cell (PBMC) suspensions were stained with Zombie UV, followed by anti-CD45RO, PD-1, CD3, ICOS, CD8, CD4, CD20, CXCR5, TIGIT and CD38 antibodies prior to analysis. …”
Publicado 2022
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15232por Muchakayala, Siva Krishna, Katari, Naresh Kumar, Saripella, Kalyan Kumar, Schaaf, Henele, Marisetti, Vishnu Murthy, Kowtharapu, Leela Prasad, Jonnalagadda, Sreekantha Babu“…We report an ideal method for quantifying impurities in mycophenolate mofetil drug substances and their oral suspension preparations. We developed a systematic and eco-friendly analytical approach utilizing quality by design (QbD) and green chemistry principles. …”
Publicado 2022
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15233por Park, Jinsung, Jo, Sungsin, Lee, Myung-Kyu, Kim, Tae-Hwan, Sung, Il-Hoon, Lee, Jin K.“…ACL-derived cells were harvested and isolated from remnants of ruptured ACLs during reconstruction surgery and cultured at passage three. Cell suspensions (200 µl) containing 2 × 10(6) ACL-derived cells or ADMSCs were prepared for the purpose of reseeding. …”
Publicado 2022
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15234por Tanaka, Isabela Vitelli, Tribst, Joao Paulo Mendes, Silva-Concilio, Lais Regiane, Bottino, Marco Antonio“…The StepWise test was performed until specimen fracture or until suspension of the test after 1.2 × 10 (6) cycles. Regarding survival, using the Kaplan–Meier method, PICN presented results for the mean and median of 245.21 N and 225 N, respectively; ZLS had an average of 175.76 N and a median of 168 N, and YZHT with an average of 383.30 N and a median of 366 N. …”
Publicado 2022
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15235“…Duration of immobility was determined using the tail suspension test and the forced swim test. Locomotor activity was evaluated in the open field test. …”
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15236“…In this contribution, we address an important application area in the domain of biotechnology, the automatic analysis of CHO-K1 suspension growth in microfluidic single-cell cultivation, where data characteristics are very dissimilar to existing domains and trained deep networks cannot easily be adapted by classical transfer learning. …”
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15237por Wardle, Heather, Donnachie, Craig, Critchlow, Nathan, Brown, Ashley, Bunn, Christopher, Dobbie, Fiona, Gray, Cindy, Mitchell, Danielle, Purves, Richard, Reith, Gerda, Stead, Martine, Hunt, Kate“…BACKGROUND: In Britain, unprecedented restrictions on daily life associated with the Covid-19 pandemic included the suspension of professional sports events during the initial ‘lockdown’. …”
Publicado 2021
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15238por Yao, Yitan, Shi, Shengya, Yang, Yating, Luo, Bei, Li, Mengdie, Zhang, Ling, Yuan, Xiaoping, Liu, Huanzhong, Zhang, Kai“…Behavior tests include novel object recognition test (NORT), elevated plus maze test (EPMT), forced swim test (FST), tail suspension test (TST), conditioned place preference (CPP). …”
Publicado 2022
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15239por Tóth, Gábor Szilveszter, Siitonen, Vilja, Nikkanen, Lauri, Sovic, Lucija, Kallio, Pauli, Kourist, Robert, Kosourov, Sergey, Allahverdiyeva, Yagut“…RESULTS: Entrapment of Synechocystis S02 cells in Ca(2+)-cross-linked alginate hydrogel beads improves the specific sucrose productivity by 86% compared to suspension cultures during 7 days of cultivation under salt stress. …”
Publicado 2022
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15240“…Our novel approach allows the investigation of suspension flow dynamics in confined systems where clogs are formed both by sieving and bridging mechanisms simultaneously. …”
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