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15501por Heathman, Thomas R. J., Glyn, Veronica A. M., Picken, Andrew, Rafiq, Qasim A., Coopman, Karen, Nienow, Alvin W., Kara, Bo, Hewitt, Christopher J.“…The hMSCs were held in suspension post‐harvest to simulate a typical pooling time for a scaled expansion process and cryopreserved in a serum‐free vehicle solution using a controlled‐rate freezing process. …”
Publicado 2015
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15502por Hughson, Andrew G., Race, Brent, Kraus, Allison, Sangaré, Laura R., Robins, Lori, Groveman, Bradley R., Saijo, Eri, Phillips, Katie, Contreras, Luis, Dhaliwal, Virkamal, Manca, Matteo, Zanusso, Gianluigi, Terry, Daniel, Williams, Jeffrey F., Caughey, Byron“…Here, we demonstrate that immersion in BrioHOCl can inactivate not only a range of target microbes, including spores of Bacillus subtilis, but also prions in tissue suspensions and on stainless steel. Real-time quaking-induced conversion (RT-QuIC) assays showed that BrioHOCl treatments eliminated all detectable prion seeding activity of human Creutzfeldt-Jakob disease, bovine spongiform encephalopathy, cervine chronic wasting disease, sheep scrapie and hamster scrapie; these findings indicated reductions of ≥10(3)- to 10(6)-fold. …”
Publicado 2016
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15503por ten Berge, Josianne C., van Rosmalen, Joost, Vermeer, Jacolien, Hellström, Cecilia, Lindskog, Cecilia, Nilsson, Peter, Qundos, Ulrika, Rothova, Aniki, Schreurs, Marco W. J.“…METHODS: An antigen suspension bead array using 188 different antigens representing 97 ocular proteins was performed to detect ARAs in serum samples of patients with presumed (n)pAIR (n = 24), uveitis (n = 151) and cataract (n = 21). …”
Publicado 2016
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15504por Turgeon, Nathalie, Michel, Kevin, Ha, Thi-Lan, Robine, Enric, Moineau, Sylvain, Duchaine, Caroline“…Aerosols were maintained in suspension during 10 minutes, 1 hour, and 2 hours. Viral aerosols were sampled using an SKC BioSampler and samples were analyzed using qPCR and plaque assays. …”
Publicado 2016
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15505por Archambault, Anne-Sophie, Turcotte, Caroline, Martin, Cyril, Lefebvre, Julie S., Provost, Véronique, Laviolette, Michel, Flamand, Nicolas“…At optimal concentration, PF-4708671 increased the half-life of LTB(4) in our neutrophil suspensions by 7.5 fold, compared to 5 fold for 17-octadecynoic acid. …”
Publicado 2017
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15506por Parslow, Roxanne M, Harris, Sarah, Broughton, Jessica, Alattas, Adla, Crawley, Esther, Haywood, Kirstie, Shaw, Alison“…Our synthesis describes four third-order constructs within children's experiences: (1) disruption and loss: physical, social and the self; (2) barriers to coping: suspension in uncertainty, problems with diagnosis and disbelief; (3) facilitators to coping: reducing uncertainty, credible illness narratives, diagnosis and supportive relationships and (4) hope, personal growth and recovery. …”
Publicado 2017
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15507por Monti, Marcello, Iommelli, Francesca, De Rosa, Viviana, Carriero, Maria Vincenza, Miceli, Roberta, Camerlingo, Rosa, Di Minno, Giovanni, Del Vecchio, Silvana“…Neutrophil-like cells stimulated with calcium ionophore (A23187) were used as a stable source of cell-free NETs-enriched suspensions. Using NETs as an adhesion substrate, two human K562 cell lines, differentially expressing α5β1 and ανβ3 integrins, were subjected to adhesion assays in the presence or absence of DNAse 1, blocking antibodies against α5β1 or ανβ3, alone or in combination with DNAse 1, and Proteinase K. …”
Publicado 2017
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15508“…Non-hypocretin-based novel treatments for narcolepsy include pitolisant (BF2.649, tiprolisant); JZP-110 (ADX-N05) for EDS in adults; JZP 13-005 for children; JZP-386, a deuterated sodium oxybate oral suspension; FT 218 an extended-release formulation of SXB; and JNJ-17216498, a new formulation of modafinil. …”
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15509por Pathak, G., Agostino, M.J., Bishara, K., Capell, W.R., Fisher, J.L., Hegde, S., Ibrahim, B.A., Pilarzyk, Kaitlyn, Sabin, C., Tuczkewycz, Taras, Wilson, Steven, Kelly, M.P.“…Consistent with the observation that lower PDE11A4 expression correlates with better lithium responsiveness, we found that Pde11a KO mice given 0.4% lithium chow for 3+ weeks exhibit greater lithium responsivity relative to WT littermates in tail suspension, an antidepressant predictive assay, and amphetamine hyperlocomotion, an anti-manic predictive assay. …”
Publicado 2016
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15510por Mercx, Sébastien, Smargiasso, Nicolas, Chaumont, François, De Pauw, Edwin, Boutry, Marc, Navarre, Catherine“…Our aim consisted of knocking-out two XylT genes and four FucT genes (12 alleles altogether) in Nicotiana tabacum BY-2 suspension cells using CRISPR/Cas9. Three XylT and six FucT sgRNAs were designed to target conserved regions. …”
Publicado 2017
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15511“…The fungal assay was performed placing a Candida albicans suspension on the PMMA surface for 2 h, then Sabouraud Dextrose Broth was added, and growth after 24 h was determined by counting colony forming units on agar plates. …”
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15512“…MATERIALS AND METHODS: Hiṅgvādi Ghṛta (HG) was prepared using standard operating procedure, physicochemically analyzed and assessed. Tail Suspension Test (TST) model with Swiss albino mice and Forced Swim Test (FST) model with Wistar albino rats were used to assess anti-depressant activity. …”
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15513por Morón-López, Sara, Puertas, Maria C., Gálvez, Cristina, Navarro, Jordi, Carrasco, Anna, Esteve, Maria, Manyé, Josep, Crespo, Manel, Salgado, Maria, Martinez-Picado, Javier“…We performed a DTT/EDTA-based treatment for epithelial layer removal followed by non-enzymatic disruption of the tissue to obtain lamina propria cell suspension (LP). CD45(+) cells were subsequently purified by flow sorting and vDNA was determined by ddPCR. …”
Publicado 2017
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15514por Venâncio, Paulo César, Raimundo Figueroba, Sidney, Dias Nani, Bruno, Eduardo Nunes Ferreira, Luiz, Vilela Muniz, Bruno, de Sá Del Fiol, Fernando, Sartoratto, Adilson, Antonio Ribeiro Rosa, Edvaldo, Carlos Groppo, Francisco“…After 24h, animals were divided: G1 (Control) – 0.5 mL of NaCl 0.9%; G2 – Asa 100 mg/kg or 400mg/kg; G3 – Atu 100 mg/kg or 400 mg/kg; G4 – amoxicillin suspension 50 mg/kg, considering PSSA infection; and G5 (Control) - 0.5 mL of NaCl 0.9%; G6 – Asa 400mg/kg; G7 – amoxicillin 50 mg/kg; and G8 - Asa 400 mg/kg + amoxicillin 50 mg/kg for MRSA. …”
Publicado 2017
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15515por Gimeno, María L., Fuertes, Florencia, Barcala Tabarrozzi, Andres E., Attorressi, Alejandra I., Cucchiani, Rodolfo, Corrales, Luis, Oliveira, Talita C., Sogayar, Mari C., Labriola, Leticia, Dewey, Ricardo A., Perone, Marcelo J.“…Muse‐AT cells evidenced high stage‐specific embryonic antigen‐3 (SSEA‐3) expression (∼60% of cells) after 7–10 days growing in suspension and did not form teratomas when injected into immunodeficient mice. …”
Publicado 2016
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15516por da Costa, Samya Thalita Picanço, Fumian, Tulio Machado, de Lima, Ian Carlos Gomes, Siqueira, Jones Anderson Monteiro, da Silva, Luciana Damascena, Hernández, Juliana das Mercês, de Lucena, Maria Silvia Souza, Reymão, Tammy Kathlyn Amaral, Soares, Luana da Silva, Mascarenhas, Joana D’Arc Pereira, Gabbay, Yvone Benchimol“…RNA was extracted from all faecal suspensions and reverse transcription-polymerase chain reaction (RT-PCR) for the NoV-polymerase partial region was performed as a trial test. …”
Publicado 2017
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15517por Cotrim, Carina Costa, Toscano, Luiza, Messias, André, Jorge, Rodrigo, Siqueira, Rubens Camargo“…The bone marrow from all patients was aspirated and processed for mononuclear cell separation. A 0.1 mL suspension of BMMF CD34(+) cells was injected into the vitreous cavity of the worse-seeing eye. …”
Publicado 2017
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15518por Wang, Cai-Xia, Chen, Yuan-Yuan, Yang, Feng, Yang, Fan, Guo, Jin-Tao, Sun, Siyu, Fan, Ling“…MATERIALS AND METHODS: The surfaces of three nCLE miniprobes were contaminated with suspensions of Bacillus atrophaeus (ATCC9372). One probe was randomly selected to count the bacterial load on the surface. …”
Publicado 2017
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15519por Deyama, Satoshi, Ishikawa, Yuka, Yoshikawa, Kotomi, Shimoda, Kento, Ide, Soichiro, Satoh, Masamichi, Minami, Masabumi“…-induced depression model mice using a tail suspension test. RESULTS: I.c.v. infusion of resolvin D1 (10 ng) and resolvin D2 (10 ng) produced antidepressant effects; these effects were significantly blocked by a resolvin D1 receptor antagonist WRW4 (10 µg, i.c.v.) and a resolvin D2 receptor antagonist O-1918 (10 µg, i.c.v.), respectively. …”
Publicado 2017
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15520por Xie, Ping, Xin, Qian, Yang, Sheng-Tao, He, Tiantian, Huang, Yuanfang, Zeng, Guangfu, Ran, Maosheng, Tang, Xiaohai“…Herein, we prepared skeleton-labeled (13)C-CNPs that had identical properties to commercial CNPs suspension injection (CNSI) for the imaging and quantification in TDLN. (13)C-CNPs were prepared by arc discharge method, followed by homogenization with polyvinylpyrrolidone. …”
Publicado 2017
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