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16221por Du, Changqing, Weng, Yingzheng, Lou, Jiangjie, Zeng, Guangzhong, Liu, Xiaowei, Jin, Hongfeng, Lin, Senna, Tang, Lijiang“…A total of 30 beagle dogs were used for establishing the MI models successfully by injecting thrombin powder and a polyethylene microsphere suspension. Serum samples were collected prior to (0 h) and following MI (1, 2 and 3 h). …”
Publicado 2019
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16222por Pang, Ye-Bin, He, Jian, Cui, Bi-Yu, Xu, Sheng, Li, Xi-Lei, Wu, Man-Ya, Liang, Rong, Feng, Yan, Guo, Xing, Zhang, Xue-Hui, Luo, Xiao-Ling“…The results of FCM analysis showed that the proportion of CD90(+)HepG2 cells in the spheral CD90(+)HepG2 enriched by suspension sphere culture was ranging from 98.7% to 99.5%, and 57.1% CD90(+)HepG2/DC fused cells were successfully constructed. …”
Publicado 2019
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16223por Guo, Chen, Xu, Ling-feng, Li, Hui-min, Wang, Wei, Guo, Ji-hua, Jia, Meng-qi, Jia, Rong, Jia, Jun“…METHODS: Anoikis-resistant CAL27 (CAL27(AR)) cells were induced from CAL27 cells using the suspension culture approach. Transcriptome analysis was performed using RNA-Seq to study the differentially expressed genes (DEGs) between the CAL27(AR)cells and the parental CAL27 cells. …”
Publicado 2019
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16224por Camilleri, Christina, Beiter, Rebecca M., Puentes, Lisett, Aracena-Sherck, Paula, Sammut, Stephen“…/misoprostol (0.3 mg/kg/ml, i.g.) or vehicle (1% Carboxymethylcellulose Sodium/0.2% Tween(®) 80 suspension, i.g.)] and pregnancy. Drug administration took place on days 12–14 of gestation (days 28–40 human gestational equivalent). …”
Publicado 2019
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16225por Vuono, David C., Lipp, Bruce, Staub, Carl, Loney, Evan, Harrold, Zoë R., Grzymski, Joseph J.“…Photo-attenuation occurs when biomass in suspension within a medium blocks and/or diffuses light directed at the detector, such that an increase in biomass results in a decrease in the measured signal. …”
Publicado 2019
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16226por Arioz, Burak I., Tastan, Bora, Tarakcioglu, Emre, Tufekci, Kemal Ugur, Olcum, Melis, Ersoy, Nevin, Bagriyanik, Alper, Genc, Kursad, Genc, Sermin“…Behavioral assessment was carried out at 24 h post-LPS injection by tail suspension and forced swimming tests. Additionally, hippocampal cytokine and NLRP3 protein levels were estimated. …”
Publicado 2019
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16227por Stellato, Mara, Czepiel, Marcin, Distler, Oliver, Błyszczuk, Przemysław, Kania, Gabriela“…Hearts were harvested and dissociated into single cell suspensions using enzymatic digestion. Cardiac cells were stained with the erythrocyte marker Ter119, the pan-leukocyte marker CD45, the endothelial cell marker CD31 and gp38 (known also as podoplanin). …”
Publicado 2019
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16228“…Four repeated intraperitoneal injections of MPTP at 17.5 mg/kg to C57BL/6 mice led to a partial reduction in the number of nigrostriatal tyrosine hydroxylase-positive neurons and to the extension of immobility time during the tail suspension test (TST), without any obvious induction of motor deficits. …”
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16229por De Francesco, Ernestina Marianna, Ózsvári, Béla, Sotgia, Federica, Lisanti, Michael P.“…By employing the 3D mammosphere assay in MCF-7 cells, we demonstrate that treatment with d-TPP dose-dependently inhibits the propagation of breast CSCs in suspension. Also, d-TPP targets adherent “bulk” cancer cells, by decreasing MCF-7 cell viability. …”
Publicado 2019
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16230por Yang, Wen-Bin, Wang, Jin-Lei, Gui, Qian, Zou, Yang, Chen, Kai, Liu, Qing, Liang, Qin-Li, Zhu, Xing-Quan, Zhou, Dong-Hui“…There was a significant increase in the levels of cytokines in the splenocyte suspensions of RH:ΔNPT1-infected mice, and a mixed Th1/Th2 response was induced in the mice. …”
Publicado 2019
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16231por Zeng, Jing, Meng, Xinyu, Zhou, Ping, Yin, Zhihua, Xie, Qinglian, Zou, Hong, Shen, Nan, Ye, Zhizhong, Tang, Yuanjia“…The elevated plus-maze test, novelty-suppressed feeding assay, open-field test, tail suspension test, social dominance tube test, three-chamber social interaction test, step-down passive avoidance test and novelty Y-maze task were used to assess behaviour. …”
Publicado 2019
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16232por Giliberti, Valeria, Polito, Raffaella, Ritter, Eglof, Broser, Matthias, Hegemann, Peter, Puskar, Ljiljana, Schade, Ulrich, Zanetti-Polzi, Laura, Daidone, Isabella, Corni, Stefano, Rusconi, Francesco, Biagioni, Paolo, Baldassarre, Leonetta, Ortolani, Michele“…For the latter, Fourier-transform infrared spectroscopy (FTIR) using difference-spectroscopy mode is typically employed, but it is performed on macroscopic liquid suspensions or thick films containing large amounts of purified photosensitive proteins. …”
Publicado 2019
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16233por Pinheiro, Larindja A. M., Pereira, Carla, Frazão, Carolina, Balcão, Victor M., Almeida, Adelaide“…Although the stability of phage φ6 was affected by UV-B and solar radiation, this can be overcome by the application of phage suspensions at the end of the day or at night.…”
Publicado 2019
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16234“…E. coli was isolated from a leftover positive urine culture specimen, cultured overnight and resuspended in phosphate-buffered saline (PBS). The suspension was run through the experimental system. …”
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16235“…Membrane proteins were extracted by successive centrifugation of bacterial suspensions (McFarland 0.5) and addition of ethanol, formic acid and acetonitrile. …”
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16236por Sauvonnet, Veronique, Fyfe, Corey, Halimi, Diane, Martelin, Roland, Zambardi, Gilles“…The ETEST ERV strip was applied on a Mueller–Hinton agar plate previously seeded with a 0.5 McF bacterial suspension. After incubation for 16–20H at 35°C, the reading was performed using the bacteriostatic mode i.e.,80% of growth. …”
Publicado 2019
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16237por Xu, Jiao-Tian, Qian, Yuan, Wang, Wei, Chen, Xiao-Xiang, Li, Yang, Li, Yu, Yang, Zhi-Yong, Song, Xiao-Bin, Lu, Di, Deng, Xing-Li“…A Parkinson’s disease rat model was injected with 6-hydroxydopamine via the right ascending nigrostriatal dopaminergic pathway, and then treated with 5 μL of neural stem cell suspension (1.5 × 10(4)/L) in the right substantia nigra. …”
Publicado 2019
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16238por Abellanas, Miguel Angel, Zamarbide, Marta, Basurco, Leyre, Luquin, Esther, Garcia-Granero, Marta, Clavero, Pedro, San Martin-Uriz, Patxi, Vilas, Amaia, Mengual, Elisa, Hervas-Stubbs, Sandra, Aymerich, Maria S.“…Microglial activation and interaction with the immune system were analyzed in single cell suspensions obtained from the different brain regions by fluorescence-activated cell sorting, next generation RNA sequencing, real-time PCR, and immunohistochemical techniques. …”
Publicado 2019
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16239“…The F(2) population obtained via the cross had been screened for blast resistance using inoculation with Pyricularia oryzae spore's suspension. The objectives of this study were to assess the presence of Pita and Pi9 genes for blast resistance and to assess the presence of the badh2 gene for aroma in the screened F(2) plants using molecular markers. …”
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16240por Ansbro, Éimhín Mary, Biringanine, Michel, Caleo, Grazia, Prieto-Merino, David, Sadique, Zia, Perel, Pablo, Jobanputra, Kiran, Roberts, Bayard“…During insecurity, control rates were initially maintained by a nurse-provided, scaled-back service, while patient volume and visit interval decreased. Following service suspension due to drug stock-outs, patients were less likely to achieve control, improving on service resumption. …”
Publicado 2019
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