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16801“…Lungs of I− mice were inoculated intranasally with bacterial suspensions of 10(7) CFU/mL; a higher inoculum of 10(9) CFU/mL was required to induce infection in I+ mice. …”
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16802por de Leeuw, Thomas G., Mangiarini, Laura, Lundin, Rebecca, Kaguelidou, Florentia, van der Zanden, Tjitske, Pasqua, Oscar Della, Tibboel, Dick, Ceci, Adriana, de Wildt, Saskia N.“…This trial is part of the European Union Seventh Framework Programme project Gabapentin in Paediatric Pain (GAPP) to develop a pediatric use marketing authorization for a new gabapentin suspension. METHODS/DESIGN: The GAPP-2 study is a randomized, double-blind, placebo-controlled, multicenter superiority phase II study in children with severe chronic neuropathic or mixed pain. …”
Publicado 2019
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16803por Heeren, A. M., Rotman, J., Stam, A. G. M., Pocorni, N., Gassama, A. A., Samuels, S., Bleeker, M. C. G., Mom, C. H., Zijlmans, H. J. M. A. A., Kenter, G. G., Jordanova, E. S., de Gruijl, T. D.“…The in-vitro effect of PD-1 blockade on T-cell reactivity to HPV16 E6 oncoproteins was determined in cultures of TDLN and PT single cell suspensions (n = 19). Also, intracellular cytokine staining (ICS) upon anti-CD3 stimulation was performed in metastatic TDLN (LN+) and PT (n = 7), as well as multiplexed immunofluorescence histochemistry staining (n = 8). …”
Publicado 2019
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16804por Locachevic, Gisele A., Prado, Morgana K. B., Zoccal, Karina F., Pereira, Priscilla A. T., Sorgi, Carlos A., Bortolanza, Mariza, Peti, Ana Paula F., Fogaça, Manoela V., Guimarães, Francisco S., Del Bel, Elaine, Faccioli, Lúcia H.“…At basal conditions, 5-LO deficiency (Alox5(−/−)) reduces the preference for sucrose, while inducing a higher immobilization time on the tail suspension test when compared 129sv. Moreover, Alox5(−/−) mice present increased caspase-1 expression and elevated levels of IL-1β, IL-17 and PGE(2) in the spleen, with increasing corticosterone levels in the frontal cortex but reducing systemic levels. …”
Publicado 2019
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16805por Li, Qiao, Ma, Lei, Shen, Shunli, Guo, Yu, Cao, Qinghua, Cai, Xiuqin, Feng, Juan, Yan, Yuan, Hu, Tianyu, Luo, Shiya, Zhou, Lin, Peng, Baogang, Yang, Zhonghan, Hua, Yunpeng“…Dysbiosis of gut microflora was induced by antibiotics (vancomycin, cefoperazone or combination of ampicillin, neomycin, metronidazole, and vancomycin). We used feces suspension to treat colonic epithelial NCM460 cells, and detected IL-25 and tuft cell marker DCLK1 using WB and immunofluorescence staining. …”
Publicado 2019
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16806por Becker, Britta, Henningsen, Lars, Paulmann, Dajana, Bischoff, Birte, Todt, Daniel, Steinmann, Eike, Steinmann, Joerg, Brill, Florian H. H., Steinmann, Jochen“…The virus-inactivating properties of surface disinfectants could be evaluated therefore with a test simulating practical conditions with mechanical action resulting in more reliable data than the existing quantitative suspension tests and/or a carrier test without any mechanical action.…”
Publicado 2019
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16807por Wu, Jian, Gao, Feng-xia, Wang, Chao, Qin, Mei, Han, Fei, Xu, Tao, Hu, Zhi, Long, Yang, He, Xue-mei, Deng, Xin, Ren, De-lian, Dai, Tian-yang“…METHODS: Single cell suspensions from blood and tissue samples were isolated by physical dissociation and filtering through a 70 μm cell strainer. …”
Publicado 2019
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16808por De Lucia, Orazio, Pierannunzii, Luca Massimo, Pregnolato, Francesca, Verduci, Elisa, Crotti, Chiara, Valcamonica, Elisabetta, Pisoni, Laura, Comi, Daniela, Lonati, Paola Adele, Meroni, Pier Luigi, Murgo, Antonella“…Pain VAS score, WOMAC, NSAID/analgesic consumption, and causes of suspension were available at 1, 6, 12, and 24 months after first administration. …”
Publicado 2019
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16809por Wurster, Sebastian, Ruiz, Oscar E, Tatara, Alexander M, Samms, Krystin M, Kim Nguyen, Anh, D. Albert, Nathaniel, H. Kahan, Philip, Mikos, Antonios G, Eisenhoffer, George T, Kontoyiannis, Dimitrios P“…After washing, larvae were incubated in Candida albicans or Rhizopus arrhizus spore suspensions (5 × 10(5)–5 × 10(7)/mL) for 16 h. Thereafter, larvae were washed again and survival was monitored until 72 h post-infection. …”
Publicado 2019
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16810“…RESULTS: Serial dilutions of both cell culture and pure oocyst suspension DNA template yielded better linearity than cell culture derived standards, with dilutions of 10(6) oocysts exhibiting similar quantification cycle (Cq) values to those obtained from DNA template dilutions of 10(6) oocysts. …”
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16811por Martinez, Fernando J, Rabe, Klaus F, Lipworth, Brian J, Arora, Samir, Jenkins, Martin, Martin, Ubaldo J, Reisner, Colin“…We pooled data from the PINNACLE-1, -2, and -4 studies to evaluate the efficacy and safety of the dual bronchodilator fixed-dose combination glycopyrrolate/formoterol fumarate metered dose inhaler (GFF MDI), formulated using co-suspension delivery technology, in GOLD category A patients with moderate-to-very severe COPD. …”
Publicado 2020
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16812por Nix, Ilka D., Idelevich, Evgeny A., Storck, Luise M., Sparbier, Katrin, Drews, Oliver, Kostrzewa, Markus, Becker, Karsten“…Comparing the different processing methods of positive BC broth, lysis/centrifugation method with a final dilution step 10(–1) of the 0.5 McFarland suspension resulted in best test performance after 4 h incubation time. …”
Publicado 2020
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16813“…Propagation in commercially formulated serum free media resulted in colony formation and growth in suspension. The addition of dimethyl sulfoxide (DMSO) or phorbol 12-myristate 13-acetate (PMA) to serum free media restored cell attachment. …”
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16814por Feng Yeh, Chia, Chih Wang, Kuo, Chai Chiang, Lien, Shieh, Den En, Hong Yen, Ming, San Chang, Jung“…The anti-HRSV activity of Radix Glycyrrhizae was further confirmed by RT-PCR and qRT-PCR. 300 μg/ml Radix Glycyrrhizae markedly decreased the viral amounts within the cells and in the suspension. Radix Glycyrrhizae might further stimulate mucosal cells to secrete IFN-β to counteract viral infection. …”
Publicado 2013
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16815por Briand, Valérie, Cottrell, Gilles, Tuike Ndam, Nicaise, Martiáñez-Vendrell, Xavier, Vianou, Bertin, Mama, Atika, Kouwaye, Bienvenue, Houzé, Sandrine, Bailly, Justine, Gbaguidi, Erasme, Sossou, Darius, Massougbodji, Achille, Accrombessi, Manfred, Mayor, Alfredo, Ding, Xavier C., Fievet, Nadine“…A total of 942 whole blood samples collected in 327 women in the 1st and 3rd trimesters and at delivery were tested by uRDT, conventional RDT (cRDT, SD BIOLINE Malaria Ag Pf), microscopy, quantitative polymerase chain-reaction (qPCR) and Luminex-based suspension array technology targeting P. falciparum HRP2. …”
Publicado 2020
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16816por Lechermann, Laura M., Manavaki, Roido, Attili, Bala, Lau, Doreen, Jarvis, Lorna B., Fryer, Tim D., Bird, Nick, Aloj, Luigi, Patel, Neel, Basu, Bristi, Cleveland, Matthew, Aigbirhio, Franklin I., Jones, Joanne L., Gallagher, Ferdia A.“…Different concentrations of labeled cell suspensions (10(4), 10(5), and 10(6) cells) were seeded on 6-well plates and into a 3 × 3 cubic-well plate with 1 cm(3) cubic wells as a gel matrix. …”
Publicado 2020
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16817por Kayuni, Sekeleghe A., O’Ferrall, Angus M., Baxter, Hamish, Hesketh, Josie, Mainga, Bright, Lally, David, Al-Harbi, Mohammad H., LaCourse, E. James, Juziwelo, Lazarus, Musaya, Janelisa, Makaula, Peter, Stothard, J. Russell“…Once control interventions can resume after coronavirus disease 2019 (COVID-19) suspensions, we recommend stepped-up preventive chemotherapy, with increased community-access to treatments, alongside renewed efforts in appropriate environmental control.…”
Publicado 2020
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16818por van Pul, Kim M, Vuylsteke, Ronald J C L M, de Beijer, Monique T A, van de Ven, Rieneke, van den Tol, M Petrousjka, Stockmann, Hein B A C, de Gruijl, Tanja D“…METHODS: Immune modulatory effects of CpG-B (CPG7909) with or without the JAK2/STAT3 inhibitor (STAT3i) AG490 were assessed in ex vivo cultured BrC SLN-derived single-cell suspensions (N=29). Multiparameter flow cytometric analyses were conducted for DC and T cell subset characterization and assessment of (intracellular) cytokine profiles. …”
Publicado 2020
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16819por Tu, Yue, Fang, Qi-Jun, Sun, Wei, Liu, Bu-Hui, Liu, Ying-Lu, Wu, Wei, Yee, Hong-Yun, Yuan, Can-Can, Wang, Mei-Zi, Wan, Zi-Yue, Tang, Ren-Mao, Wan, Yi-Gang, Tang, Hai-Tao“…METHODS: In vivo, the CRF rat models were induced by uninephrectomy, potassium oxonate, and proinflammatory diet, and received either TFA suspension, FEB, or vehicle after modeling for 28 days. …”
Publicado 2020
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16820por de Camargo Reis Mello, Daphne, Rodrigues, Lais Morandini, D’Antola Mello, Fabia Zampieri, Gonçalves, Thais Fernanda, Ferreira, Bento, Schneider, Sandra Giacomin, de Oliveira, Luciane Dias, de Vasconcellos, Luana Marotta Reis“…For monotypic biofilm formation, standardized suspensions (10(6) cells/ml) with the microorganisms S. aureus (S.a) and P. aeruginosa (P.a) were cultured for 24 h on the samples and submitted to an MTT test. …”
Publicado 2020
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