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17061por Rivera-Irizarry, Jean K., Zallar, Lia J., Levine, Olivia B., Skelly, Mary Jane, Boyce, Jared E., Barney, Thaddeus, Kopyto, Ruth, Pleil, Kristen E.“…There was no effect of alcohol history on stress coping and negative affective behaviors (sucrose preference, forced swim test, tail suspension) in either sex. CONCLUSION: Female mice engaged in higher volume binge drinking than their male counterparts. …”
Publicado 2023
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17062por Donlin, Laura T., Rao, Deepak A., Wei, Kevin, Slowikowski, Kamil, McGeachy, Mandy J., Turner, Jason D., Meednu, Nida, Mizoguchi, Fumitaka, Gutierrez-Arcelus, Maria, Lieb, David J., Keegan, Joshua, Muskat, Kaylin, Hillman, Joshua, Rozo, Cristina, Ricker, Edd, Eisenhaure, Thomas M., Li, Shuqiang, Browne, Edward P., Chicoine, Adam, Sutherby, Danielle, Noma, Akiko, Nusbaum, Chad, Kelly, Stephen, Pernis, Alessandra B., Ivashkiv, Lionel B., Goodman, Susan M., Robinson, William H., Utz, Paul J., Lederer, James A., Gravallese, Ellen M., Boyce, Brendan F., Hacohen, Nir, Pitzalis, Costantino, Gregersen, Peter K., Firestein, Gary S., Raychaudhuri, Soumya, Moreland, Larry W., Holers, V. Michael, Bykerk, Vivian P., Filer, Andrew, Boyle, David L., Brenner, Michael B., Anolik, Jennifer H.“…In parallel, each sample was flow sorted into fibroblast, T-cell, B-cell, and macrophage suspensions for bulk population RNA-seq and plate-based single-cell CEL-Seq2 RNA-seq. …”
Publicado 2018
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17063por Ubillos, Itziar, Ayestaran, Aintzane, Nhabomba, Augusto J, Dosoo, David, Vidal, Marta, Jiménez, Alfons, Jairoce, Chenjerai, Sanz, Hèctor, Aguilar, Ruth, Williams, Nana Aba, Díez-Padrisa, Núria, Mpina, Maximilian, Sorgho, Hermann, Agnandji, Selidji Todagbe, Kariuki, Simon, Mordmüller, Benjamin, Daubenberger, Claudia, Asante, Kwaku Poku, Owusu-Agyei, Seth, Sacarlal, Jahit, Aide, Pedro, Aponte, John J, Dutta, Sheetij, Gyan, Ben, Campo, Joseph J, Valim, Clarissa, Moncunill, Gemma, Dobaño, Carlota“…METHODS: We measured total IgM, IgG, and IgG(1–4) subclass antibodies to three constructs of the Plasmodium falciparum circumsporozoite protein (CSP) and hepatitis B surface antigen (HBsAg) that are part of the RTS,S vaccine, by quantitative suspension array technology. Plasma and serum samples were analyzed in 195 infants and children from two sites in Ghana (Kintampo) and Mozambique (Manhiça) with different transmission intensities using a case-control study design. …”
Publicado 2018
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17064“…The spleen of mice was taken to prepare single-cell suspension, and the percentage of T lymphocytes in spleen to CD8(+)T cells was detected. …”
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17065por Goyal, Vikas, Grimwood, Keith, Byrnes, Catherine A, Morris, Peter S, Masters, I Brent, Ware, Robert S, McCallum, Gabrielle B, Binks, Michael J, Marchant, Julie M, van Asperen, Peter, O'Grady, Kerry-Ann F, Champion, Anita, Buntain, Helen M, Petsky, Helen, Torzillo, Paul J, Chang, Anne B“…At the start of an exacerbation, children were randomly assigned to oral suspensions of either amoxicillin–clavulanate (22·5 mg/kg, twice daily) and placebo or azithromycin (5 mg/kg per day) and placebo for 21 days. …”
Publicado 2018
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17066por Seifer, David B., Petok, William D., Agrawal, Alisha, Glenn, Tanya L., Bayer, Arielle H., Witt, Barry R., Burgin, Blair D., Lieman, Harry J.“…BACKGROUND: On March 17, 2020 an expert ASRM task force recommended the temporary suspension of new, non-urgent fertility treatments during an ongoing world-wide pandemic of Covid-19. …”
Publicado 2021
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17067por Vecchi, Luigi, Bonomini, Mario, Palumbo, Roberto, Arduini, Arduino, Borrelli, Silvio“…During low-sodium CAPD, antihypertensive therapy (amlodipine 10 mg and Olmesartan 20 mg) has been reduced until complete suspension. After 6 months, we repeated ABPM showing a substantial reduction in mean 24 h-ABP (117/69 mmHg), mean diurnal ABP (119/75 mmHg) and mean nocturnal ABP (111/70 mmHg). …”
Publicado 2021
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17068por Ploner, Christian, Rauchenwald, Tina, Connolly, Catherine E., Joehrer, Karin, Rainer, Johannes, Seifarth, Christof, Hermann, Martin, Nagl, Markus, Lobenwein, Susanne, Wilflingseder, Doris, Cappellano, Giuseppe, Morandi, Evi M., Pierer, Gerhard“…RESULTS: Single and clustered adipocyte populations were detected in 11 out of 13 granulation tissue specimens and single-cell suspensions from granulation tissue showed adipogenic differentiation potential. …”
Publicado 2021
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17069por Gaspe, María Sol, Cardinal, Marta Victoria, Fernández, María del Pilar, Vassena, Claudia Viviana, Santo-Orihuela, Pablo Luis, Enriquez, Gustavo Fabián, Alvedro, Alejandra, Laiño, Mariano Alberto, Nattero, Julieta, Alvarado-Otegui, Julián Antonio, Macchiaverna, Natalia Paula, Cecere, María Carla, Freilij, Héctor, Gürtler, Ricardo Esteban“…Following community mobilization and school-based interventions, the 2015–2019 program assessed baseline house infestation using a stratified sampling strategy; sprayed all rural houses with suspension concentrate beta-cypermethrin, and selectively sprayed infested and adjacent houses in urban and peri-urban settings; and monitored house infestation and performed selective treatments over the follow-up. …”
Publicado 2021
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17070por Egorov, Andrey I., Converse, Reagan, Griffin, Shannon M., Bonasso, Russell, Wickersham, Lindsay, Klein, Elizabeth, Kobylanski, Jason, Ritter, Rebecca, Styles, Jennifer N., Ward, Honorine, Sams, Elizabeth, Hudgens, Edward, Dufour, Alfred, Wade, Timothy J.“…Samples were analyzed for immunoglobulin (Ig) G responses to recombinant antigens of three noroviruses and Cryptosporidium, as well as protein purification tags as internal controls, using an in-house multiplex suspension immunoassay on the Luminex platform. Responses were defined as ratios of antibody reactivities with a target protein and its purification tag. …”
Publicado 2021
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17071“…After being incubated with a bacterial suspension for 4 weeks, the specimen were irrigated with 2% NaOCl activated by conventional needle irrigation (CNI) (n = 10) or PIPS (n = 10). …”
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17072por Byrne, Eoin, Björkmalm, Johanna, Bostick, James P., Sreenivas, Krishnan, Willquist, Karin, van Niel, Ed W. J.“…When co-cultured with the adapted C. saccharolyticus G5 mixed culture at a hydraulic retention time (HRT) of 20 h, C. owensensis constituted only 0.09–1.58% of the population in suspension. CONCLUSIONS: The adaptation of members of the Caldicellulosiruptor genus to higher sugar concentrations established that the ability to develop improved strains via ALE is species dependent, with C. owensensis adapted to grow on 80 g/L, whereas C. kristjanssonii could only be adapted to 30 g/L glucose. …”
Publicado 2021
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17073por Li, Yanzhen, Zhang, Hong, Yang, Jingwen, Zhan, Muouyang, Hu, Xuefei, Liu, Yongmin, Yu, Lingling, Yan, Xiaochen, Liang, Shangdong, Zhang, Ruyue, Lu, Ying, Li, Beining, Liu, Cunzhi, Li, Man“…The sucrose preference test, tail suspension test and forced swimming test were used to evaluate depression in mice. …”
Publicado 2021
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17074por Bisanzio, Donal, Reithinger, Richard, Alqunaibet, Ada, Almudarra, Sami, Alsukait, Reem F., Dong, Di, Zhang, Yi, El-Saharty, Sameh, Herbst, Christopher H.“…BACKGROUND: The Kingdom of Saudi Arabia (KSA) quickly controlled the spread of SARS-CoV-2 by implementing several non-pharmaceutical interventions (NPIs), including suspension of international and national travel, local curfews, closing public spaces (i.e., schools and universities, malls and shops), and limiting religious gatherings. …”
Publicado 2022
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17075por Kenney, H. Mark, Wu, Chia-Lung, Loiselle, Alayna E., Xing, Lianping, Ritchlin, Christopher T., Schwarz, Edward M.“…METHODS: Single-cell suspensions of PLVs were sorted for smooth muscle cells (SMCs), which was validated by Cspg4-Cre;tdTomato reporter gene expression. …”
Publicado 2022
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17076por Lin, Qimei, Cao, Jiasong, Du, Xiaoling, Yang, Kuo, Yang, Xu, Liang, Zhixian, Shi, Jiandang, Zhang, Ju“…The high-performance liquid chromatography-mass spectrometry assay was carried out to examine intracellular 4-OHE2 levels. Serum-free suspension culture and flow cytometry assays were performed to evaluate PCSCs. …”
Publicado 2022
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17077por Grüntkemeier, Lisa, Khurana, Aditi, Bischoff, Farideh Zamaniyan, Hoffmann, Oliver, Kimmig, Rainer, Moore, Mathew, Cotter, Philip, Kasimir-Bauer, Sabine“…METHODS: 50 µm FFPE tumor curls from the validation cohort (n = 49) and the development cohort (n = 25) underwent cutting, deparaffinization and antigen retrieval followed by dissociation into a single-cell suspension. After staining for cytokeratin, vimentin, DAPI and separation via DEPArray™, single cells were processed for HER2-FISH analysis to assess the number of chromosome 17 and HER2 loci signals for comparison, either with available IHC or conventional tissue section FISH. …”
Publicado 2022
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17078por Moore, Craig, Wong, Arnold Y. L., de Luca, Katie, De Carvalho, Diana, Johansson, Melker S., Pohlman, Katherine A., Miller, Amy, Funabashi, Martha, Dougherty, Paul, French, Simon, Adams, Jon, Kawchuk, Greg“…While findings varied by region, most chiropractors (85%) indicated that they had implemented regulator advice on the use of personal protective equipment (PPE). Suspension of face-to-face patient care during the peak of the pandemic was reported by 49% of the participants with 26% implementing telehealth since the pandemic began. …”
Publicado 2022
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17079por Jiang, Xue, Yi, Saini, Liu, Qin, Su, Dapeng, Li, Liangyuan, Xiao, Chenghong, Zhang, Jinqiang“…Depression-like behaviors were assessed in the forced swimming test (FST), sucrose preference test (SPT), tail suspension test (TST). Microglial phenotypes were evaluated using immunofluorescence staining, real-time quantitative PCR and enzyme-linked immunosorbent assays in hippocampus of mice. …”
Publicado 2022
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17080por Tsymbalyuk, Orest, Gerzanich, Volodymyr, Simard, J. Marc, Rathinam, Chozha Vendan“…Skin biopsies were digested in the presence of liberase + DNase I. Single cell suspensions were made, red blood cells were lysed with Ammonium chloride (Stem Cell Technology) and subsequently filtered using a 70 μM nylon mesh. …”
Publicado 2022
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