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6321“…Apoptosis was detected via quantitative terminal deoxynucleotidyl transferase (TdT) deoxyuridine triphosphate nick-end labelling (TUNEL) staining, caspase-3 activity assay, and detection of Bcl-2 and Bax protein expression by immunohistochemistry and Western blotting. …”
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6322por Ham, Onju, Lee, Se-Yeon, Lee, Chang Youn, Park, Jun-Hee, Lee, Jiyun, Seo, Hyang-Hee, Cha, Min-Ji, Choi, Eunhyun, Kim, Soonhag, Hwang, Ki-Chul“…Let-7b-transfected MSCs (let-7b-MSCs) showed high expression of survival-related proteins, including p-MEK, p-ERK and Bcl-2, leading to a decrease in Annexin V/PI- and TUNEL-positive cells under ROS-rich conditions. Moreover, autophagy-related genes, including Atg5, Atg7, Atg12 and beclin-1, were significantly downregulated in let-7b-MSCs. …”
Publicado 2015
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6323por Li, Jichen, Yang, Dezhao, Wang, Wei, Piao, Songlin, Zhou, Jianyu, Saiyin, Wuliji, Zheng, Changyu, Sun, Hongchen, Li, Yu“…Apoptosis in vivo was analyzed by TUNEL assay. HE staining was used to observe the morphology of the samples. …”
Publicado 2015
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6324por Fujii, Tomomi, Shimada, Keiji, Tatsumi, Yoshihiro, Hatakeyama, Kinta, Obayashi, Chiho, Fujimoto, Kiyohide, Konishi, Noboru“…Cellular senescence and apoptosis were measured by senescence-associated β-galactosidase (SA-β-gal) and TUNEL assay, respectively. Quantitative RT-PCR was used to measure mRNA expression of various genes, including syndecan-1, stem cell factors, and markers of differentiation into squamous, glandular, or neuroendocrine cells. …”
Publicado 2015
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6325por Mátyás, Csaba, Németh, Balázs Tamás, Oláh, Attila, Hidi, László, Birtalan, Ede, Kellermayer, Dalma, Ruppert, Mihály, Korkmaz-Icöz, Sevil, Kökény, Gábor, Horváth, Eszter Mária, Szabó, Gábor, Merkely, Béla, Radovits, Tamás“…Cardiac structure, markers of fibrotic remodelling and DNA damage were examined by histology, immunohistochemistry and TUNEL assay, respectively. RESULTS: DM was associated with deteriorated cGMP signalling in the myocardium (elevated phosphodiesterase-5 expression, lower cGMP-level and impaired PKG activity). …”
Publicado 2015
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6326por Kim, Chang Seong, Kim, In Jin, Bae, Eun Hui, Ma, Seong Kwon, Lee, JongUn, Kim, Soo Wan“…Ang-(1–7) also significantly ameliorated TUNEL positive cells in UUO kidneys. Additionally, Ang-(1–7) reduced profibrotic protein expression and decreased the increased tumor growth factor (TGF)-β1/Smad signaling present in UUO kidneys. …”
Publicado 2015
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6327por Ma, Sihui, Huang, Di, Zhai, Mengxin, Yang, Lubing, Peng, Sen, Chen, Changxu, Feng, Xiaoru, Weng, Qiang, Zhang, Bolin, Xu, Meiyu“…Cell cycle distribution, Annexin V-FITC/PI double staining, TUNEL assay, western blot and immunofluorescence for LC3-II assay were used to detect apoptosis and autophagy on cells. …”
Publicado 2015
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6328por Chakrabarti, Gaurab, Silvers, Molly A., Ilcheva, Mariya, Liu, Yuliang, Moore, Zachary R., Luo, Xiuquan, Gao, Jinming, Anderson, Glenda, Liu, Lili, Sarode, Venetia, Gerber, David E., Burma, Sandeep, DeBerardinis, Ralph J., Gerson, Stanton L., Boothman, David A.“…MeOX + ß-lapachone exposure resulted in elevated DNA double-strand breaks, PARP1 hyperactivation and TUNEL+ programmed necrosis. Combination treatment caused dramatic antitumor activity, enhanced PARP1-hyperactivation in tumor tissue, and improved survival of mice bearing MiaPaca2-derived xenografts, with 33% apparent cures. …”
Publicado 2015
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6329por Yeong, Looi Ting, Abdul Hamid, Roslida, Saiful Yazan, Latifah, Khaza’ai, Huzwah, Mohtarrudin, Norhafizah“…Upon termination, histopathological and biochemical analysis, as well as Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and transcription factor enzyme-linked immunosorbent assay (ELISA) assays were performed to elucidate the potential mechanism of TQF. …”
Publicado 2015
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6330por Zhao, Yu Tina, Du, Jianfeng, Chen, Youfang, Tang, Yaoliang, Qin, Gangjian, Lv, Guorong, Zhuang, Shougang, Zhao, Ting C.“…Terminal deoxynucleotidyltransferase (TdT) nick-end labeling (TUNEL) was carried out to assess apoptotic cardiomyocytes. …”
Publicado 2015
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6331“…We also ascertained the ability of these drugs to modulate cell death as induced by live Bb in HSC using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) viability assay and the potential of dexamethasone to modulate Bb-induced apoptosis in HSC by the TUNEL assay. RESULTS: Earlier, we reported that dexamethasone significantly reduced Bb-induced immune mediators and apoptosis in rhesus DRG cell cultures. …”
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6332por Binder, April K., Kosak, Justin P., Janhardhan, Kyathanahalli S., Moser, Glenda, Eling, Thomas E., Korach, Kenneth S.“…Examination of the mammary glands in these mice suggests that hNAG-1 expressing mice have altered mammary epithelial development during pregnancy, including reduced occupancy of the fat pad and increased apoptosis via TUNEL positive cells on lactation day 2. Pups nursing from hNAG-1 expressing dams have reduced milk spots compared to pups nursing from WT dams. …”
Publicado 2016
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6333por Luan, Yang, Wang, Tao, Ruan, Yajun, Jiang, Hongyang, Chen, Ruibao, Ling, Qing, Li, Mingchao, Wang, Shaogang, Liu, Jihong, Ye, Zhangqun“…In addition, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was conducted to calculate the apoptosis index (AI) of cells in CC. …”
Publicado 2015
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6334por Ganbat, Dariimaa, Seehase, Sophie, Richter, Elvira, Vollmer, Ekkehard, Reiling, Norbert, Fellenberg, Kurt, Gaede, Karoline I., Kugler, Christian, Goldmann, Torsten“…Viability of ex vivo stimulated tissues demonstrated by TUNEL-assay was acceptable. Serial sections verified sufficient diffusion of the infectious agents deep into the tissues. …”
Publicado 2016
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6335por Wang, Tao, Zhang, Shui-Jun, Cao, Sheng-Li, Guo, Wen-Zhi, Yan, Bing, Fang, Hong-Bo“…Hepatic cell apoptosis was detected using TUNEL. The results were analyzed using Quantity-one v4.62 software (Bio-Rad, USA). …”
Publicado 2015
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6336por Chang, Lihong, Huang, Jiancong, Wang, Kai, Li, Jingjia, Yan, Ruicheng, Zhu, Ling, Ye, Jin, Wu, Xifu, Zhuang, Shimin, Li, Daqing, Zhang, Gehua“…Nude mice subcutaneous injection, tumor growth curve and TUNEL assay were used to evaluate tumor regression and apoptosis in vivo. …”
Publicado 2016
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6337por Santos, Marília Harumi Higuchi, de Lourdes Higuchi, Maria, Tucci, Paulo J F, Garavelo, Shérrira M, Reis, Márcia M, Antonio, Ednei L, Serra, Andrey J, Maranhão, Raul Cavalcante“…Echocardiography was employed to detect left ventricular function and the infarct size. Additionally, the TUNEL technique was used to assess apoptosis and immunohistochemistry was used to quantitatively analyze the PPAR-α, TNF-α and NF-κB antigens in the infarcted and non-infarcted myocardium. …”
Publicado 2016
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6338por LEE, HAE NIM, JANG, HYE YEON, KIM, HYEONG JIN, SHIN, SEONG AH, CHOO, GANG SIK, PARK, YOUNG SEOK, KIM, SANG KI, JUNG, JI YOUN“…The α-mangostin-treated nude mice bearing YD-15 tumor xenografts exhibited a significantly reduced tumor volume and tumor weight due to the potent promoting effects of α-mangostin on cancer cell apoptosis, as determined by TUNEL assay. Immunohistochemical analysis revealed that the level of cleaved caspase-3 increased, whereas the Ki-67, p-ERK1/2 and p-p38 levels decreased in the α-mangostin-treated mice. …”
Publicado 2016
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6339por Wilkinson, Richard D. A., Young, Andrew, Burden, Roberta E., Williams, Rich, Scott, Christopher J.“…Subsequent immunohistochemical staining of proliferation (Ki67) and apoptosis (TUNEL) was carried out on MCF7 tumors. RESULTS: We confirmed that this inhibitor was able to selectively target cathepsin S over family members K, V, L and B. …”
Publicado 2016
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6340por Liu, Xiangchun, Liu, Haiying, Sun, Lina, Chen, Zhixin, Nie, Huibin, Sun, Aili, Liu, Gang, Guan, Guangju“…Only 24.3 ± 1.5 % of BrdU(+) LRCs co-expressed with Ki67 and 9.1 ± 1.4 % of BrdU(+) LRCs were positive for TUNEL following renal I/R injury. Interestingly, we found that newly regenerated cells formed a niche-like structure and LRCs in pairs tended to locate in this structure, but the number of those LRCs was very low. …”
Publicado 2016
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