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6441por Subirada, Paula V., Paz, María C., Ridano, Magali E., Lorenc, Valeria E., Fader, Claudio M., Chiabrando, Gustavo A., Sánchez, María C.“…In line with these results, TUNEL staining showed a slight increase in the number of positive cells in the outer nuclear layer at P17 OIR. …”
Publicado 2019
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6442por Jia, Jia, Gong, Xiaoying, Zhao, Yang, Yang, Zhenyu, Ji, Kaiqiang, Luan, Ting, Zang, Bin, Li, Guofu“…Cell apoptosis was examined using TUNEL staining. ALA treatment improved the survival rate, restored the loss of body weight and pro-inflammatory cytokines production in the serum of CLP-induced septic rats. …”
Publicado 2019
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6443“…The average bloody ascitic volumes collected from untreated, Res-in-ethanol, and Res-in-DMSO groups were 5.65±0.27, 2.75±0.14, and 2.09±0.11 ml, respectively. Abundant TUNEL-positive cells, ARHI and PIAS3 upregulation, CA125 reduction, and decreased STAT3 nuclear translocation were found in the Res-in-ethanol and, especially, the Res-in-DMSO group. …”
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6444“…In Atg5(flox/flox); Nestin-Cre mice, RPE and glial cells were the main targets of viral infection, and number of EA positive retinal cells and TUNEL positive retinal cells was significantly increased compared to Atg5(flox/+); Nestin-Cre mice though there was no difference of virus propagation between Atg5(flox/flox); Nestin-Cre mice and Atg5(flox/+); Nestin-Cre mice. …”
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6445por Wei, Di, Zhang, Geng, Zhu, Zheng, Zheng, Yu, Yan, Fei, Pan, Chongxian, Wang, Zhiyong, Li, Xian, Wang, Fuli, Meng, Ping, Zheng, Wanxiang, Yan, Zhao, Zhai, Dongsheng, Lu, Zifan, Yuan, Jianlin“…The cell cycle was analyzed by flow cytometry, whereas apoptosis was analyzed using flow cytometry and a terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay. Protein expression was examined by Western blot and immunofluorescence. …”
Publicado 2019
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6446por Jo, Min Jee, Jeong, Soyeon, Yun, Hye Kyeong, Kim, Dae Yeong, Kim, Bo Ram, Kim, Jung Lim, Na, Yoo Jin, Park, Seong Hye, Jeong, Yoon A., Kim, Bu Gyeom, Ashktorab, Hassan, Smoot, Duane T., Heo, Jun Young, Han, Jeongsu, Lee, Dae-Hee, Oh, Sang Cheul“…METHODS: MTT assays were performed to determine the cell viability of gastric cancer and gastric epithelial cell lines (AGS, MKN45, SNU638, MKN74, HFE-145). A TUNEL assay and Western blotting were carried out to investigate apoptosis. …”
Publicado 2019
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6447por Chen, Jiunn-Liang, Hung, Chun-Tzu, Keller, Joseph Jordan, Lin, Hsien-Chung, Wu, Yu-Jen“…Visual inspection was first achieved with inverted light microscopy and then the DeadEnd™ Fluorometric TUNEL System was used to observe nuclear DNA fragmentation. …”
Publicado 2019
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6448por Liu, Yunen, Tong, Changci, Xu, Ying, Cong, Peifang, Liu, Ying, Shi, Lin, Shi, Xiuyun, Zhao, Yan, Bi, Guangyuan, Jin, Hongxu, Hou, Mingxiao“…Apoptosis and oxidative stress were evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and reactive oxygen species (ROS). …”
Publicado 2019
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6449“…Neurological function and cerebral water content were determined, and cerebral infarct volume was evaluated by TTC staining. TUNEL staining was used to detect the apoptosis of nerve cells. …”
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6450“…TdT-mediated dUTP nick end labeling (TUNEL) staining and flow cytometry analysis were applied to detect the apoptotic cell number after miR-506 was downregulated in RB cells. …”
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6451por Oria, Marc, Duru, Soner, Figueira, Rebeca L., Scorletti, Federico, Turner, Lucas E., Fernandez-Alonso, Irati, Fernandez-Martin, Alejandra, Marotta, Mario, Sbragia, Lourenco, Shaaban, Aimen F., Peiro, Jose L.“…Apoptosis was measured by TUNEL assay and PI/AV flow cytometry. Valproic acid at E8 induced exencephaly in 22% of fetuses. …”
Publicado 2019
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6452por Jiao, Ying-Qian, Huang, Ping, Yan, Li, Sun, Kai, Pan, Chun-Shui, Li, Quan, Fan, Jing-Yu, Ma, Zhi-Zhong, Han, Jing-Yan“…Neuronal damage and apoptosis were assessed by Nissl staining and TUNEL staining. To assess the mechanisms of cerebrovascular hyperpermeability, we performed immunofluorescence and Western blot to assess the expression and integrity of cerebral microvascular tight junction (TJ) and caveolin-1 (Cav-1) in cortex. …”
Publicado 2019
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6453por Jia, Chang, Zhang, Jian, Chen, Huanwen, Zhuge, Yingzhi, Chen, Huiqiao, Qian, Fanyu, Zhou, Kailiang, Niu, Chao, Wang, Fangyan, Qiu, Huixian, Wang, Zhenquan, Xiao, Jian, Rong, Xing, Chu, Maoping“…In vitro, exposure of human umbilical vein endothelial cells (HUVECs) to KD sera-treated THP1 cells resulted in the activation of NLRP3 inflammasome and subsequent pyroptosis induction, as evidenced by elevated expression of caspase-1, GSDMD, cleaved p30 form of GSDMD, IL-1β and IL-18, and increased LDH release and TUNEL and propidium iodide (PI)-positive cells. Furthermore, our results showed that NLRP3-dependent endothelial cell pyroptosis was activated by HMGB1/RAGE/cathepsin B signaling. …”
Publicado 2019
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6454“…Apoptosis was measured by flow cytometry analysis and TUNEL staining, and protein expression was detected by Western blotting and immunohistochemistry. …”
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6455“…The number of apoptotic cells in the different groups was also identified using TUNEL. RESULTS: Results suggested that VAS causes reduction in myocardial necrosis by reduction of elevated LDH, CK-MB, and TnT levels. …”
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6456por Zhou, Xichang, Wang, Xiang, Zhou, Yizhou, Cheng, Long, Zhang, Youwei, Zhang, Yangmei“…To determine cell activities, CCK-8, flow cytometry, invasion assays, and TUNEL assays were performed. Real-time PCR, Western blot, and luciferase reporter assays were performed to investigate the related mechanism of HCC. …”
Publicado 2019
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6457por Bassiri, Farzaneh, Nasr-Esfahani, Mohammad Hossein, Forozanfar, Mohsen, Tavalaee, Marziyeh“…Sperm parameters, DNA damage, protamine de- ficiency, persistent histones, and lipid peroxidation were assessed using computer-assisted sperm analysis (CASA) system, sperm chromatin structure assay (SCSA) and Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays, chromomycin A3, aniline blue, and BODIPY C11 staining, respectively. …”
Publicado 2020
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6458por Fei, Xiaowei, He, Yeting, Chen, Jia, Man, Weitao, Chen, Chen, Sun, Kai, Ding, Boyun, Wang, Chongwu, Xu, Ruxiang“…The effects of TLR4 on apoptosis were observed by TUNEL, Hoechst, and HE staining techniques. The association with TLR4 in inflammation and apoptosis was explored using IL-1β and TNF-α antagonists. …”
Publicado 2019
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6459por Li, Guanghui, Wang, Xi, Li, Chunmei, Hu, Shuang, Niu, Zhixing, Sun, Qiang, Sun, Minglei“…Apoptosis in OSCC cells and xenografts was determined using a TUNEL assay kit. The activity of caspase-3, caspase-8 and caspase-1 in OSCC cells was measured with colorimetric caspase assay kits. …”
Publicado 2019
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6460“…MATERIALS AND METHODS: ANG II infusion was used to establish the hypertensive model in HT22 cells and 129S6/SvEvTac mice, respectively. TUNEL assay was used to evaluate the apoptosis level. …”
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