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Análisis vectorial
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Insectos vectores en las plantas
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81181“…Meanwhile, the expression of miR-450b-3p in GC cell lines was verified using qRT-PCR. miR-450b-3p overexpression vectors was constructed in GC cell lines including AGS and BGC-823, and then CCK-8 cell proliferation assay, Plate colony formation assay and EdU assay were applied to analyze the biological function of miR-450b-3p in GC cell lines. …”
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81182por Zhang, Yun, Zhang, Yixin, Tian, Ke, Wang, Yunfeng, Fan, Xiaoping, Pan, Qi, Qin, Guangcheng, Zhang, Dunke, Chen, Lixue, Zhou, Jiying“…FluoroGold (FG) and CTB-555 were selected as retrograde tracers and injected into the VN and TNC, respectively. Lentiviral vectors comprising CGRP short hairpin RNA (LV-CGRP) was injected into the trigeminal ganglion. …”
Publicado 2020
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81183por Faiman, Roy, Yaro, Alpha S., Diallo, Moussa, Dao, Adama, Djibril, Samake, Sanogo, Zana L., Sullivan, Margery, Krishna, Asha, Krajacich, Benjamin J., Lehmann, Tovi“…METHODS: A novel tethered-flight assay was used to characterize flight in the three primary malaria vectors- An. arabiensis, An. coluzzii and An. gambiae s.s. …”
Publicado 2020
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81184por Sakowicz, Allie, Ayala, Alexander E., Ukeje, Chideraa C., Witting, Celeste S., Grobman, William A., Miller, Emily S.“…The observed association between severe acute respiratory syndrome coronavirus 2 infection and having children raises the possibility of children themselves being vectors of viral spread or behavior patterns of parents being mediators of acquisition.…”
Publicado 2020
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81185por Stifter, Katja, Krieger, Jana, Ruths, Leonie, Gout, Johann, Mulaw, Medhanie, Lechel, Andre, Kleger, Alexander, Seufferlein, Thomas, Wagner, Martin, Schirmbeck, Reinhold“…METHODS: We generated tumor antigen-expressing vectors, immunized TAP-competent/-deficient mice and determined de novo primed CD8 T-cell frequencies by flow cytometry. …”
Publicado 2020
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81186“…In FVTLDA, its major novelty lies in the integration of direct and indirect features related to lncRNA-disease associations such as the feature vectors of lncRNA-disease pairs and their corresponding association probability fractions, which guarantees that FVTLDA can be utilized to predict diseases without known related-lncRNAs and lncRNAs without known related-diseases. …”
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81187por Mbuba, Emmanuel, Odufuwa, Olukayode G., Tenywa, Frank C., Philipo, Rose, Tambwe, Mgeni M., Swai, Johnson K., Moore, Jason D., Moore, Sarah J.“…This study evaluated the protective efficacy and protection duration of a new topical repellent ointment containing 15% DEET, MAÏA(®) compared to 20% DEET in ethanol using malaria and dengue mosquito vectors in Bagamoyo Tanzania. METHODS: Fully balanced 3 × 3 Latin square design studies were conducted in large semi-field chambers using laboratory strains of Anopheles gambiae sensu stricto, Anopheles arabiensis and Aedes aegypti. …”
Publicado 2021
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81188por Fashae, Kayode, Engelmann, Ines, Monecke, Stefan, Braun, Sascha D., Ehricht, Ralf“…The role of animals as carriers and vectors of multi-resistant bacteria in different geographical regions is poorly understood. …”
Publicado 2021
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81189por Xiao, Pengcheng, Zhu, Zhenglin, Du, Chengcheng, Zeng, Yongsheng, Liao, Junyi, Cheng, Qiang, Chen, Hong, Zhao, Chen, Huang, Wei“…Transgenic expression of BMP2 and silencing of Smad7 and Smad7 was achieved by adenoviral vectors. The osteogenic differentiation was detected by alkaline phosphatase staining, alizarin red staining, and RT-PCR analysis of the osteogenic genes RUNX2, Osterix, and Osteocalcin. …”
Publicado 2021
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81190por Bilusic, Marijo, McMahon, Sheri, Madan, Ravi A, Karzai, Fatima, Tsai, Yo-Ting, Donahue, Renee N, Palena, Claudia, Jochems, Caroline, Marté, Jennifer L, Floudas, Charalampos, Strauss, Julius, Redman, Jason, Abdul Sater, Houssein, Rabizadeh, Shahrooz, Soon-Shiong, Patrick, Schlom, Jeffrey, Gulley, James L“…A novel vaccine platform using adenovirus 5 (Ad5) vectors [E1–, E2b–] targeting three TAAs—prostate-specific antigen (PSA), brachyury, and MUC-1—has been developed. …”
Publicado 2021
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81191por Bouma, Gerrit J, Ali, Asghar, Hord, Taylor K, Parsons, Agata M, Anthony, Russell Vernon, Bruemmer, Jason E, Winger, Quinton A“…Current experiments are ongoing to determine the effects of KDM1A and AR knockdown using shRNA lentiviral target vectors on conceptus elongation and pregnancy. Collectively these results indicate that KDM1A plays a central role in regulating genes necessary for trophoblast cell proliferation. …”
Publicado 2021
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81192por Keup, Corinna, Suryaprakash, Vinay, Hauch, Siegfried, Storbeck, Markus, Hahn, Peter, Sprenger-Haussels, Markus, Kolberg, Hans-Christian, Tewes, Mitra, Hoffmann, Oliver, Kimmig, Rainer, Kasimir-Bauer, Sabine“…SVD revealed that the strongest singular vectors originate from all four LBAs, but a majority originated from CTC gDNA. …”
Publicado 2021
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81193por Grisales, Nelson, Lees, Rosemary S., Maas, James, Morgan, John C., Wangrawa, Dimitri W., Guelbeogo, Wamdaogo M., N’Fale, Sagnon, Lindsay, Steven W., McCall, Philip J., Ranson, Hilary“…CONCLUSION: Exposure to a mixture of PPF and pyrethroids on netting shortens the lifespan of mosquitoes and reduces reproductive output. Sterilization of vectors lasted at least one year under operational conditions. …”
Publicado 2021
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81194por Wang, Xuening, Chen, Chih-Hsiung, Badeti, Saiaditya, Cho, Jong Hyun, Naghizadeh, Alireza, Wang, Ziren, Liu, Dongfang“…These cell lines were transduced with either wild-type (WT-S) S protein or a mutated variant where the ER-retention motif was removed (Δ19-S), as well as human ACE2 expression vectors. Different co-culture combinations of spike-expressing 293T, A549, K562, and SK-Hep1 cells with hACE2-expressing cells revealed cell hybrid fusion. …”
Publicado 2021
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81195“…This study aims to explore the function and clinical relevance of UBE2T in lung cancer. METHODS: Lentiviral vectors were used to mediate UBE2T depletion or overexpress UBE2T in lung cancer cells. …”
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81196por Zhan, Fangbiao, He, Tao, Chen, Zhiyu, Zuo, Qiang, Wang, Yang, Li, Qiaochu, Zhong, Shenxi, Ou, Yunsheng“…OS cell lines (HOS and MG63) in which RhoA and YAP were knocked down or overexpressed were generated using lentiviral vectors. CCK-8 assays were used to examine OS cell viability, while the apoptotic death of these cells was monitored via Hoechst staining, Western blotting, and flow cytometry. …”
Publicado 2021
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81197por Wang, Hongda, Zhao, Yiming, Ren, Bingbing, Qin, Yafei, Li, Guangming, Kong, Dejun, Qin, Hong, Hao, Jingpeng, Sun, Daqing, Wang, Hao“…In vitro, ERCs were transfected with lentivirus vectors carrying LGALS9 gene and encoding green fluoresce protein (GFP-Gal-9-LVs) at a MOI 50, Gal-9 expression in ERCs was detected by ELISA and Q-PCR. …”
Publicado 2021
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81198por Williams, Mark S., Basma, Naseer J., Amaral, Fabio M. R., Wiseman, Daniel H., Somervaille, Tim C. P.“…Sorted blasts were subjected to RNA sequencing. Lentiviral vectors expressing short hairpin RNAs were used to assess the effect of FOXM1 knockdown on colony forming capacity, proliferative capacity and apoptosis in cell lines, primary AML cells and CD34+ cells from healthy donors. …”
Publicado 2021
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81199por Preciado, Silvia, Sirerol-Piquer, Mª Salomé, Muntión, Sandra, Osugui, Lika, Martí-Chillón, Gerardo J., Navarro-Bailón, Almudena, Sepúlveda, Pilar, Sánchez-Guijo, Fermín“…Lentiviral overexpression of HIF-1α was performed transducing cells with pWPI-green fluorescent protein (GFP) (MSC WT) or pWPI-HIF-1α-GFP (HIF-MSC) expression vectors. Human cord blood CD34(+) cells were co-cultured with MSC WT or HIF-MSC (4:1) for 72 h. …”
Publicado 2021
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81200“…Western blotting and immunofluorescence analysis were used to detect progesterone receptor membrane component 1 (PGRMC1), light chain 3 beta 2 (LC3B2) and sequestosome 1 (SQSTM1/p62) levels. Lentiviral vectors or siRNAs transfection, and fluorescent probes staining were used to explore the underlying mechanism. …”
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