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  1. 128961
    “…Cell death was observed by TUNEL staining, and iron accumulation in kidneys of mice was detected by Prussian blue staining. Western blotting, immunohistochemistry, and immunofluorescence were used to investigate the presence of inflammation, oxidative stress, mitochondrial dysfunction, iron deposition, and lipid peroxidation in mouse kidneys. …”
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    Shenshuaifu Granule Attenuates Acute Kidney Injury by Inhibiting Ferroptosis Mediated by p53/SLC7A11/GPX4 Pathway
  2. 128962
    “…Primary outcomes are severity of hip pain while walking using an 11-point numeric rating scale and physical function using the Western Ontario and McMaster Universities Osteoarthritis Index subscale. …”
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    A self-directed digital exercise program for hip osteoarthritis (“My Hip Exercise”): protocol for a randomised controlled trial
  3. 128963
    “…The binding mechanism between the compounds and the p53 protein was investigated with molecular docking and validated with cellular thermal shift assay (CETSA). Western blotting (WB) was employed to detect the expression of p53 protein and apoptosis-related proteins in cells. …”
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    Prenylated chromones and flavonoids isolated from the roots of Flemingia macrophylla and their anti-lung cancer activity
  4. 128964
    “…Expressions of miR-499-5p, TGF-β1, smad2, α-SMA, collagen-I and TGFβ-R1 in mRNA and protein level were subsequently detected via quantitative real-time polymerase chain reaction and western blot. Furthermore, the prediction of the binding sites of miR-499-5p and TGFβ-R1 was performed via the bioinformatics online software TargetScan and verified by dual luciferase reporter. …”
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    MicroRNA-499-5p inhibits transforming growth factor-β1-induced Smad2 signaling pathway and suppresses fibroblast proliferation and collagen synthesis in rat by targeting TGFβ-R1
  5. 128965
    “…Immunohistochemistry staining was performed to evaluate CD31‐positive vessel density and stromal cell‐derived factor‐l alpha (SDF‐1α) expression in the MI heart in vivo and the expression and distribution of SDF‐1α, C‐X‐C motif chemokine receptor 4 and CXCR7 in HCAECs in vitro. Western blotting was used to detect the levels of SDF‐1α, V‐akt murine thymoma viral oncogene homolog (AKT), phosphorylated AKT (pAKT), specificity protein 1 (Sp1) and phosphorylation of Sp1 in HCAECs. …”
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    Vagus nerve stimulation‐induced stromal cell‐derived factor‐l alpha participates in angiogenesis and repair of infarcted hearts
  6. 128966
    “…PD-L1 levels were detected by PCR, western blotting and flow cytometry. RNA-seqencing, immunoprecipitation, chromatin immunoprecipitation and transmission electron microscopy were used to elucidate the regulatory mechanism in HCC with different TP53 status. …”
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    TP53/mTORC1-mediated bidirectional regulation of PD-L1 modulates immune evasion in hepatocellular carcinoma
  7. 128967
    “…METHODS: Experimental methods including qRT‒PCR, immunofluorescence, flow cytometry, western blot, co-immunoprecipitation, CCK-8, colony formation assay, in vitro cell transwell migration and invasion assays, in vivo xenograft tumor experiments, liver and lung CRC metastasis models, CAC mice models, transmission electron microscopy, immunohistochemistry, histopathology, 4D label-free proteomics sequencing, bioinformatic and database analysis were used in this study. …”
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    Musashi-2 Deficiency Triggers Colorectal Cancer Ferroptosis by Downregulating the MAPK Signaling Cascade to Inhibit HSPB1 Phosphorylation
  8. 128968
    “…METHODS: Growth inhibitory assays were performed in AML cell lines and Ba/F3 cells expressing various FLT3 mutants to evaluate the antitumor activity of GNF-7 in vitro. Western blotting was used to examine the inhibitory  effect of GNF-7 on FLT3 and its downstream pathways. …”
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    GNF-7, a novel FLT3 inhibitor, overcomes drug resistance for the treatment of FLT3‑ITD acute myeloid leukemia
  9. 128969
    “…The expression of RAC1 was detected using western blot, RT-PCR and RNA sequencing. ELISA and flow cytometry were utilized to measure reactive oxygen species (ROS) generation upon tigecycline treatment and flow cytometry to detect alterations in cell cycle. …”
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    Tigecycline causes loss of cell viability mediated by mitochondrial OXPHOS and RAC1 in hepatocellular carcinoma cells
  10. 128970
    “…Africa region had a significantly lower fully vaccination coverage (aRR 0.71, 95% CI 0.36—0.54) and received at least one dose of the COVID-19 vaccine (aRR 0.78, 95% CI 0.62—0.99) than Europe region. Conversely, the Western Pacific region had significantly higher fully vaccination coverage (aRR 1.40 95% CI 1.12—1.74) and received at least one dose of COVID-19 vaccines (aRR 1.40 95% CI 1.14—1.73) relative to European region. …”
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    Association between Gross National Income per capita and COVID-19 vaccination coverage: a global ecological study
  11. 128971
    “…Because of the growing proportion of elderly people in Western industrialized societies, the number of these fractures can be expected to increase further in the coming years, and with it the burden on healthcare resources. …”
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    Patients with femoral or distal forearm fracture in Germany: a prospective observational study on health care situation and outcome
  12. 128972
    “…The ENAR(® )(Electro Neuro Adaptive Regulator) therapy device combines Western biofeedback with Eastern energy medicine. …”
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    The effectiveness of ENAR(® )for the treatment of chronic neck pain in Australian adults: a preliminary single-blind, randomised controlled trial
  13. 128973
    “…A polyclonal antibody raised against denatured DNase I was shown by Western blotting to stain a 32-kD band after enrichment of the endonuclease from day 0 and 3 homogenates by preparative gel electrophoresis. …”
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    Androgen Ablation Leads to an Upregulation and Intranuclear Accumulation of Deoxyribonuclease I in Rat Prostate Epithelial Cells Paralleling Their Apoptotic Elimination
  14. 128974
    “…Reverse transcription polymerase chain reaction (RT–PCR) and real time PCR were used to detect the mRNA expression. Western blot and immunohistochemistry analysis were applied to examine the protein expression and distribution. …”
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    Differential expression of the catalytic subunits for PP-1 and PP-2A and the regulatory subunits for PP–2A in mouse eye
  15. 128975
    “…No MMP-1 protein could be found using Western blotting. MMP-2 mRNA and protein were present in all cell lines, with the highest expression of both latent and active MMP-2 in the highest metastatic cell lines MV3 and BLM. …”
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    Matrix metalloproteinases in human melanoma cell lines and xenografts: increased expression of activated matrix metalloproteinase-2 (MMP-2) correlates with melanoma progression
  16. 128976
    “…Epidermal growth factor receptor expression, p53 status and p42/p44 (for testing a constitutively active mitogen-activated protein kinase pathway status) were determined by competition analysis (Scatchard plots), denaturing gradient cell electrophoresis and Western blot, respectively. Epidermal growth factor receptor levels ranged from 388 to 33794 fmol mg(−1) protein, a range that is similar to that found in head and neck tumours. …”
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    Influence of epidermal growth factor receptor (EGFR), p53 and intrinsic MAP kinase pathway status of tumour cells on the antiproliferative effect of ZD1839 (‘Iressa’)
  17. 128977
    por Nosè, Michela, Accordini, Simone, Artioli, Paola, Barale, Francesco, Barbui, Corrado, Beneduce, Rossella, Berardi, Domenico, Bertolazzi, Gerardo, Biancosino, Bruno, Bisogno, Alfredo, Bivi, Raffaella, Bogetto, Filippo, Boso, Marianna, Bozzani, Alberto, Bucolo, Piera, Casale, Marcello, Cascone, Liliana, Ciammella, Luisa, Cicolini, Alessia, Cipresso, Gabriele, Cipriani, Andrea, Colombo, Paola, Dal Santo, Barbara, De Francesco, Michele, Di Lorenzo, Giorgio, Di Munzio, Walter, Ducci, Giuseppe, Erlicher, Arcadio, Esposito, Eleonora, Ferrannini, Luigi, Ferrato, Farida, Ferro, Antonio, Fragomeno, Nicoletta, Parise, Vincenzo Fricchione, Frova, Maria, Gardellin, Francesco, Garzotto, Nicola, Giambartolomei, Andrea, Giupponi, Giancarlo, Grassi, Luigi, Grazian, Natalia, Grecu, Lorella, Guerrini, Gualtiero, Laddomada, Francesco, Lazzarin, Ermanna, Lintas, Camilla, Malchiodi, Francesca, Malvini, Lara, Marchiaro, Livio, Marsilio, Alessandra, Mauri, Massimo Carlo, Mautone, Antonio, Menchetti, Marco, Migliorini, Giuseppe, Mollica, Marco, Moretti, Daniele, Mulè, Serena, Nicholau, Stylianos, Nosè, Flavio, Occhionero, Guglielmo, Pacilli, Anna Maria, Pecchioli, Stefania, Percudani, Mauro, Piantato, Ennio, Piazza, Carlo, Pontarollo, Francesco, Pycha, Roger, Quartesan, Roberto, Rillosi, Luciana, Risso, Francesco, Rizzo, Raffella, Rocca, Paola, Roma, Stefania, Rossattini, Matteo, Rossi, Giuseppe, Rossi, Giovanni, Sala, Alessandra, Santilli, Claudio, Saraò, Giuseppe, Sarnicola, Antonio, Sartore, Francesca, Scarone, Silvio, Sciarma, Tiziana, Siracusano, Alberto, Strizzolo, Stefania, Tansella, Michele, Targa, Gino, Tasser, Annamarie, Tomasi, Rodolfo, Travaglini, Rossana, Veronese, Antonio, Ziero, Simona
    Publicado 2009
    “…The employed pragmatic attitude allowed to randomly allocate more than 100 individuals, which means that this study is the largest antipsychotic combination trial conducted so far in Western countries. We expect that the current project, by generating evidence on whether it is clinically useful to combine clozapine with aripiprazole rather than with haloperidol, provides physicians with a solid evidence base to be directly applied in the routine care of patients with schizophrenia. …”
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    Rationale and design of an independent randomised controlled trial evaluating the effectiveness of aripiprazole or haloperidol in combination with clozapine for treatment-resistant schizophrenia
  18. 128978
    “…BACKGROUND: Lung cancer is one of the leading causes of death in Europe and the western world. At present, diagnosis of lung cancer very often happens late in the course of the disease since inexpensive, non-invasive and sufficiently sensitive and specific screening methods are not available. …”
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    Noninvasive detection of lung cancer by analysis of exhaled breath
  19. 128979
    “…Low back pain (LBP), a major health problem in Western countries, can be caused by a variety of pathologies, one of which is degenerative disc disease (DDD). …”
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    Total disc replacement surgery for symptomatic degenerative lumbar disc disease: a systematic review of the literature
  20. 128980
    “…METHODS: STAT6 protein expression was analyzed by Western blotting in GBM cell lines and by immunohistochemistry in a tissue microarray (TMA) of glioma patient tissues. …”
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    STAT6 expression in glioblastoma promotes invasive growth
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