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129941por Ranjit, Sanjay“…We observed that ERAP1+/- mice had 45% lower ERAP1 levels by western blot analyses in heart and kidney as compared to wild-type (WT) littermate control mice maintained on regular rodent chow (n= 10 of each type, p=0.029). …”
Publicado 2019
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129942por Song, Yichen, Shao, Lianqi, Xue, Yixue, Ruan, Xuelei, Liu, Xiaobai, Yang, Chunqing, Zheng, Jian, Shen, Shuyuan, Chen, Jiajia, Li, Zhen, Liu, Yunhui“…METHODS: Quantitative RT-PCR and western blot analysis were employed to detect the expression levels of A1CF, lncRNA family with sequence similarity 224 member A (FAM224A), miR-590-3p, zinc finger protein 143 (ZNF143) and ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3) in glioma tissues and cell lines. …”
Publicado 2019
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129943por Loh, Jing Loong Moses, Chan, Stephrene, Wong, Keng Lin, de Mel, Sanjay, Yap, Eng Soo“…This is in contrast to recent studies that showed higher post-operative VTE rates similar to Western populations. In addition, patients who were administered chemoprophylaxis did not have a statistically significant difference in incidence of VTE although it did show a correlation with higher post-operative outcome scores which may indicate better function. …”
Publicado 2019
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129944por Pickering, Amy J., Njenga, Sammy M., Steinbaum, Lauren, Swarthout, Jenna, Lin, Audrie, Arnold, Benjamin F., Stewart, Christine P., Dentz, Holly N., Mureithi, MaryAnne, Chieng, Benard, Wolfe, Marlene, Mahoney, Ryan, Kihara, Jimmy, Byrd, Kendra, Rao, Gouthami, Meerkerk, Theodora, Cheruiyot, Priscah, Papaiakovou, Marina, Pilotte, Nils, Williams, Steven A., Colford, John M., Null, Clair“…METHODS AND FINDINGS: We enrolled geographic clusters of pregnant women in rural western Kenya into a cluster-randomized controlled trial (ClinicalTrials.gov NCT01704105) that tested 6 interventions: water treatment, improved sanitation, handwashing with soap, combined water treatment, sanitation, and handwashing (WSH), improved nutrition, and combined WSH and nutrition (WSHN). …”
Publicado 2019
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129945por Li, Si-Si, Jiang, Wei-Liang, Xiao, Wen-Qin, Li, Kai, Zhang, Ye-Fei, Guo, Xing-Ya, Dai, Yi-Qi, Zhao, Qiu-Yan, Jiang, Ming-Jie, Lu, Zhan-Jun, Wan, Rong“…The protein and mRNA levels of relevant factors were analyzed using Western blotting, immunofluorescence and real time-quantitative PCR. …”
Publicado 2019
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129946por Wang, Chenlong, Chen, Yaxin, Wang, Yang, Liu, Xiaoxiao, Liu, Yanzhuo, Li, Ying, Chen, Honglei, Fan, Chengpeng, Wu, Dongfang, Yang, Jing“…The expression of CD31 and α-SMA was measured by immunofluorescence. Western blot was used to measure the expression of HIF-1, Akt and p-Akt. …”
Publicado 2019
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129947por Zhao, Yuechao, Li, Yiran, Sheng, Jie, Wu, Fan, Li, Kai, Huang, Rong, Wang, Xiaojuan, Jiao, Tao, Guan, Xin, Lu, Yan, Chen, Xiao, Luo, Zhiwen, Zhou, Yanchi, Hu, Hanjie, Liu, Wenjie, Du, Boyu, Miao, Shiying, Cai, Jianqiang, Wang, Linfang, Zhao, Hong, Ying, Jianming, Bi, Xinyu, Song, Wei“…Transwell, spheroid formation, fluorescence activated cell sorter (FACS), xenograft nude mouse model, tumor frequency assessed by extreme limiting dilution analysis (ELDA), Western blot assays and chemoresistance analysis were performed to elucidate the functions and possible mechanism of lnc273–31 and lnc273–34 in cancer stem cells. …”
Publicado 2019
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129948por van der Pluijm, Rob W, Imwong, Mallika, Chau, Nguyen Hoang, Hoa, Nhu Thi, Thuy-Nhien, Nguyen Thanh, Thanh, Ngo Viet, Jittamala, Podjanee, Hanboonkunupakarn, Borimas, Chutasmit, Kitipumi, Saelow, Chalermpon, Runjarern, Ratchadaporn, Kaewmok, Weerayuth, Tripura, Rupam, Peto, Thomas J, Yok, Sovann, Suon, Seila, Sreng, Sokunthea, Mao, Sivanna, Oun, Savuth, Yen, Sovannary, Amaratunga, Chanaki, Lek, Dysoley, Huy, Rekol, Dhorda, Mehul, Chotivanich, Kesinee, Ashley, Elizabeth A, Mukaka, Mavuto, Waithira, Naomi, Cheah, Phaik Yeong, Maude, Richard J, Amato, Roberto, Pearson, Richard D, Gonçalves, Sónia, Jacob, Christopher G, Hamilton, William L, Fairhurst, Rick M, Tarning, Joel, Winterberg, Markus, Kwiatkowski, Dominic P, Pukrittayakamee, Sasithon, Hien, Tran Tinh, Day, Nicholas PJ, Miotto, Olivo, White, Nicholas J, Dondorp, Arjen M“…PCR-corrected efficacies for individual sites were 12·7% (2·2–33·0) in northeastern Thailand, 38·2% (15·9–60·5) in western Cambodia, 73·4% (57·0–84·3) in Ratanakiri (northeastern Cambodia), and 47·1% (33·5–59·6) in Binh Phuoc (southwestern Vietnam). …”
Publicado 2019
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129949por Wang, Hai-Yang, Li, Yi, Li, Jia-Jia, Jiao, Chun-Hua, Zhao, Xiao-Jing, Li, Xue-Ting, Lu, Mei-Jiao, Mao, Xia-Qiong, Zhang, Hong-Jie“…Serum food-specific IgG antibodies were detected by semi-quantitative enzyme-linked immunosorbent assay, and serum food-specific IgE antibodies were measured by Western blot. The value of food-specific IgG antibodies was compared among different groups, and potent factors related to these antibodies were explored by binary logistic regression. …”
Publicado 2019
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129950por Du, Yiri, Cui, Hongwei, Xiao, Yunfeng, Li, Jianbo, Su, Engboer, Xu, Zhipeng, Mi, Weidong“…Data will be captured through gene expression in POCD rats via Quantitative Real-Time PCR (QRT-PCR). On the other side, Western Blot was used to measure the expression levels of IRS-2, p-IRS-2, p-PI3K, PI3K, p-AKT, AKT, GLUT4, and p-GLUT4. …”
Publicado 2019
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129951por Chandramouli, Chanchal, Tay, Wan Ting, Bamadhaj, Nurul Sahiddah, Tromp, Jasper, Teng, Tiew-Hwa Katherine, Yap, Jonathan J. L., MacDonald, Michael R., Hung, Chung-Lieh, Streng, Koen, Naik, Ajay, Wander, Gurpreet Singh, Sawhney, Jitendra, Ling, Lieng Hsi, Richards, A. Mark, Anand, Inder, Voors, Adriaan A., Lam, Carolyn S. P.“…Data on the ‘obesity paradox’, reported in Western populations, are scarce in Asia and have only utilised the traditional classification of body mass index (BMI). …”
Publicado 2019
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129952por Donald, Kirsten Ann, Wedderburn, Catherine J., Barnett, Whitney, Nhapi, Raymond T., Rehman, Andrea M., Stadler, Jacob A. M., Hoffman, Nadia, Koen, Nastassja, Zar, Heather J., Stein, Dan J.“…METHODS AND FINDINGS: The Drakenstein Child Health Study is a population-based birth cohort in the Western Cape, South Africa. Pregnant women were enrolled between 20 and 28 weeks’ gestation from two community clinics from 2012 to 2015; sociodemographic and psychosocial data were collected antenatally. …”
Publicado 2019
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129953“…The correlation of miR-145, TNF-α, IL-6, and IL-10 levels with visual analogue scale (VAS), Lysholm, and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scores was assessed by Pearson correlation analysis. …”
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129954por Owens, Jake L, Beketova, Elena, Tinsley, Samantha, Asberry, Andrew, Deng, Xuehong, Hu, Chang-Deng“…We then used RNA-seq, qPCR, western blot, and ChIP to evaluate a potential epigenetic role of PRMT5 in activating the expression of genes critical to DSB repair. …”
Publicado 2019
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129955por Huaman, Moises, Qualls, Joseph E., Kuhel, David, Jose, Shinsmon, Konaniah, Eddy, Komaravolu, Ravi, Fichtenbaum, Carl J., Deepe, George S., Hui, David Y.“…Mice were subsequently fed a western-type diet (WD) containing 21% fat and 0.2% cholesterol for 16 weeks. …”
Publicado 2019
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129956por Fang, Jiankang, Zhao, Xia, Li, Shuai, Xing, Xingan, Wang, Haitao, Lazarovici, Philip, Zheng, Wenhua“…The effects of artemisinin on protein expression of BMSCs including p-Erk1/2, t-Erk1/2, p-c-Raf, p-p90(rsk), p-CREB, BCL-2, Bax, p-Akt, t-Akt, β-actin, and GAPDH were measured by western blotting. RESULTS: We characterized for the first time the protective effect of artemisinin, an anti-malaria drug, using oxidative stress-induced apoptosis in vitro, in rat BMSC cultures. …”
Publicado 2019
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129957por Wang, Ya, Cheng, Tingting, Lu, Miaolong, Mu, Yun, Li, Biao, Li, Xuejun, Zhan, Xianquan“…Cytoscape was used to obtain most significant modules. Western blot was used to validate the expressions of upregulated proteins (ITGA1, ITGA6, and ITGB4), the expression and phosphorylated status of Akt in PI3K-Akt pathway, and the expression of FSH receptors in FSH-positive relative to negative NFPAs. …”
Publicado 2019
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129958por Sayers, Sophie R., Beavil, Rebecca L., Fine, Nicholas H. F., Huang, Guo C., Choudhary, Pratik, Pacholarz, Kamila J., Barran, Perdita E., Butterworth, Sam, Mills, Charlotte E., Cruickshank, J. Kennedy, Silvestre, Marta P., Poppitt, Sally D., McGill, Anne-Thea, Lavery, Gareth G., Hodson, David J., Caton, Paul W.“…Total, monomeric and dimeric eNAMPT and nicotinamide mononucleotide (NMN) were evaluated by ELISA, western blot and fluorometric assay using serum from non-diabetic, glucose intolerant and type 2 diabetic individuals. …”
Publicado 2019
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129959por Yan, Dong, Zhao, Li-li, Yue, Bo-wen, Qian, Hui, Zhang, Zi-han, Wang, Ning, Yan, Shi-hai, Qian, Yu-liang“…The effects of Granule of BU-XIN RUAN-MAI on isoprenaline-induced myocardial cell injury were determined by conducting H&E staining and by performing ELISA to examine the serum content of MDA, SOD, Na(+)/K(+)-ATPase, cAMP, and the content of inflammatory factors in isoprenaline-induced rats. Meanwhile, western blot and real time PCR were used to determine the expression of genes involved in oxidation and energy metabolism, and real time PCR was also used for determination of miR-542-3p expression. …”
Publicado 2019
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129960por Chang, Jie, Yang, Bobo, Zhou, Yun, Yin, Changsheng, Liu, Tingting, Qian, Hai, Xing, Guangwei, Wang, Suhua, Li, Fang, Zhang, Yubin, Chen, Da, Aschner, Michael, Lu, Rongzhu“…[Formula: see text] , and its downstream proteins, glucose transporter 1 (GLUT-1), erythropoietin (EPO), and vascular endothelial growth factor A (VEGF-A) were analyzed by means of Western blotting. Real-time PCR was used to detect the expression of [Formula: see text] mRNA. …”
Publicado 2019
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