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129961por Wang, Lixin, Cao, Ying, Guan, Zhizhong, Luo, Guangheng, Luo, Lei, Yang, Xiushu, Chu, Mingliang“…Overexpressed CKIP-1 human prostate epithelial cells (BPH-1 cells) were established and the expression of TGF-β(1) was detected by Western blotting. Furthermore, a non-contact co-culture system of BPH-1 cells and human fibroblast (HFF-1) cells was used to observe the effects of BPH-1 cell and their high CKIP-1 levels on the expression of TGF-β(1) in HFF-1 in vitro. …”
Publicado 2019
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129962por Zeng, Xin, Zhu, Min, Liu, Xiaohong, Chen, Xuanmin, Yuan, Yujia, Li, Lan, Liu, Jingping, Lu, Yanrong, Cheng, Jingqiu, Chen, Younan“…The expressions of pyroptosis and ER stress related genes or proteins were determined by real-time PCR, Western blot or immunofluorescence. The morphology of pyroptosis was detected by acridine orange and ethidium bromide (AO/EB) staining. …”
Publicado 2020
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129963“…Then quantitative polymerase chain reaction was performed to quantify the expression of miR-19a-3p and FOXF2 in HT29 and HCT116 cells, and western blot (WB) analysis was conducted to evaluate the levels of FOXF2, glycogen synthase kinase 3 beta (GSK-3β), phosphorylated GSK-3β (p-GSK-3β), β-catenin, p-β-catenin, α-catenin, N-cadherin, E-cadherin, and vimentin. …”
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129964“…Expression of vascular endothelial growth factor A (VEGFA) and tumor necrosis factor-stimulated gene-6 (TSG-6) in hADSCs and MTs were identified by Western blotting. Then the ID-MTs were employed for 3D bioprinting. …”
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129965por Gańczak, Maria, Miazgowski, Tomasz, Kożybska, Marta, Kotwas, Artur, Korzeń, Marcin, Rudnicki, Bartosz, Nogal, Tomasz, Andrei, Catalina Liliana, Ausloos, Marcel, Banach, Maciej, Brazinova, Alexandra, Constantin, Maria-Magdalena, Dubljanin, Eleonora, Herteliu, Claudiu, Hostiuc, Mihaela, Hostiuc, Sorin, Jakovljevic, Mihajlo, Jozwiak, Jacek Jerzy, Kissimova-Skarbek, Katarzyna, Król, Zbigniew J., Mestrovic, Tomislav, Miazgowski, Bartosz, Milevska Kostova, Neda, Naghavi, Mohsen, Negoi, Ionut, Negoi, Ruxandra Irina, Pana, Adrian, Rubino, Salvatore, Sekerija, Mario, Sierpinski, Radoslaw, Szponar, Lucjan, Topor-Madry, Roman, Vujcic, Isidora S., Widecka, Justyna, Widecka, Katarzyna, Wojtyniak, Bogdan, Zadnik, Vesna, Kopec, Jacek A.“…While the health gap between Poland and Western Europe has diminished, it remains substantial. …”
Publicado 2020
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129966por Du, Huaidong, Guo, Yu, Bennett, Derrick A., Bragg, Fiona, Bian, Zheng, Chadni, Mahmuda, Yu, Canqing, Chen, Yiping, Tan, Yunlong, Millwood, Iona Y., Gan, Wei, Yang, Ling, Yao, Pang, Luo, Guojin, Li, Jianguo, Qin, Yulu, Lv, Jun, Lin, Xu, Key, Tim, Chen, Junshi, Clarke, Robert, Li, Liming, Chen, Zhengming“…AIMS/HYPOTHESIS: Previous evidence linking red meat consumption with diabetes risk mainly came from western countries, with little evidence from China, where patterns of meat consumption are different. …”
Publicado 2020
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129967“…The mRNA expression levels of miR-124, Hedgehog, Patched (Ptch), Smoothened (Smo), and glioma-associated oncogene homolog (Gli) in liver tissues were determined through RT-PCR, and the protein levels of Hedgehog, Ptch, Smo, Gli, P13k, Akt, HMGB1, TLR4, IkB-α, p-IkB-α, and NF-kB65 were evaluated via Western blot analysis. The serum levels of IL-6, TNF-α, CRP, IL-12, and ICAM-1 were determined via ELISA. …”
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129968por Ferrer, Isidro, Andrés-Benito, Pol, Zelaya, Maria Victoria, Aguirre, Maria Elena Erro, Carmona, Margarita, Ausín, Karina, Lachén-Montes, Mercedes, Fernández-Irigoyen, Joaquín, Santamaría, Enrique, del Rio, José Antonio“…Immunohistochemistry, western blotting, transcriptomic, proteomics and phosphoproteomics, and intra-cerebral inoculation of brain homogenates to wild-type (WT) mice were the methods employed. …”
Publicado 2020
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129969“…A cross-sectional study was carried out from November 2016 to May 2017 to identify the major ectoparasites species and potential risk factors in Welkait district western part of Tigray region. A total of 102 sheep and 324 goats were physically examined and samples were taken for laboratory analysis. …”
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129970por Uwase, Juliette, Chu, Ruilin, Kassegne, Kokouvi, Lei, Yao, Shen, Feihu, Fu, Haitian, Sun, Yifan, Xuan, Yinghua, Cao, Jun, Cheng, Yang“…The specificity of anti-MSP4-immunoglobulin (Ig) G antibodies was evaluated by Western blot and enzyme-linked immunosorbent assays, and cellular immune responses were analysed via lymphocyte proliferation assays. …”
Publicado 2020
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129971por Chen, Jia-Liang, Zhou, Xin, Liu, Bo-Long, Wei, Xu-Hong, Ding, Hong-Lu, Lin, Zhi-Jun, Zhan, Hai-Lun, Yang, Fei, Li, Wen-Biao, Xie, Jun-Cong, Su, Min-Zhi, Liu, Xian-Guo, Zhou, Xiang-Fu“…Concentrations of Mg(2+) in serum and cerebrospinal fluid (CSF) were measured by calmagite chronometry. Western blot and immunofluorescence staining measured the expression of tumor necrosis factor-α/nuclear factor-κB (TNF-α/NF-κB), interleukin-1β (IL-1β), and N-methyl-d-aspartate receptor type 2B subunit (NR2B) of the N-methyl-d-aspartate receptor in the L6–S1 spinal dorsal horn (SDH) and hippocampus. …”
Publicado 2020
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129972por Zhang, Yufeng, Lyu, Chunming, Fong, Sophia Yui Kau, Wang, Qian, Li, Chenrui, Ho, Nicolas James, Chan, Kay Sheung, Yan, Xiaoyu, Zuo, Zhong“…To continue our study on the pharmacokinetic and pharmacodynamic interactions between Wen Bing formulas and oseltamivir (OS), the first-line western drug for the treatment of influenza, further interactions between OS and the eight single herbs and their relevant marker components from Wen Bing formulas were investigated in the current study. …”
Publicado 2019
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129973por Tucker, Bradford S., Campbell, Richard E., Tjoumakaris, Fotios P., Freedman, Kevin B., Miller, Lawrence S., Maria, Daniel Santa, Leider, Morgan, Garza, Jamie“…Knee function was assessed using the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score. …”
Publicado 2020
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129974por Yan, Mei-Ling, Zhang, Shuai, Zhao, Hong-Mei, Xia, Sheng-Nan, Jin, Zhuo, Xu, Yi, Yang, Lin, Qu, Yang, Huang, Si-Yu, Duan, Ming-Jing, Mao, Meng, An, Xiao-Bin, Mishra, Chandan, Zhang, Xin-Yu, Sun, Li-Hua, Ai, Jing“…METHODS: In the present study, we performed a battery of techniques, including primary neuronal culture, patch clamp, stereotaxic injection of the lentiviral vectors, morris water maze (MWM), dual luciferase reporter assay, FM1–43 fluorescence dye evaluation, qRT-PCR and western blot, to investigate the regulatory effect of miR-153 on hippocampal synaptic vesicle release both in vivo and in vitro. …”
Publicado 2020
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129975por Weisser, Burkhard, Predel, Hans-Georg, Gillessen, Anton, Hacke, Claudia, vor dem Esche, Johannes, Rippin, Gerd, Noetel, Andrea, Randerath, Olaf“…INTRODUCTION: Cardiovascular diseases (CVD) represent the first cause of mortality in western countries. Hypertension and dyslipidemia are strong risk factors for CVD, and are prevalent either alone or in combination. …”
Publicado 2020
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129976por Phillips, Richard O, Robert, Jérôme, Abass, Kabiru Mohamed, Thompson, William, Sarfo, Fred Stephen, Wilson, Tuah, Sarpong, Godfred, Gateau, Thierry, Chauty, Annick, Omollo, Raymond, Ochieng Otieno, Michael, Egondi, Thaddaeus W, Ampadu, Edwin O, Agossadou, Didier, Marion, Estelle, Ganlonon, Line, Wansbrough-Jones, Mark, Grosset, Jacques, Macdonald, John M, Treadwell, Terry, Saunderson, Paul, Paintsil, Albert, Lehman, Linda, Frimpong, Michael, Sarpong, Nanaa Francisca, Saizonou, Raoul, Tiendrebeogo, Alexandre, Ohene, Sally-Ann, Stienstra, Ymkje, Asiedu, Kingsley B, van der Werf, Tjip S“…It is most prevalent in western and central Africa and Australia. Standard antimicrobial treatment with oral rifampicin 10 mg/kg plus intramuscular streptomycin 15 mg/kg once daily for 8 weeks (RS8) is highly effective, but streptomycin injections are painful and potentially harmful. …”
Publicado 2020
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129977por Ma, Bingqiang, Ma, Jianxun, Yang, Yili, He, Xueyuan, Pan, Xinmin, Wang, Zhan, Qian, Yaowen“…QRT-PCR was employed to assess the miR-330-3p and PRRX1 expressions in the samples, and the cell expressions of PRRX1, GSK-3β, p-GSK-3β, β-catenin, p-β-catenin, cyclin D1, N-cadherin, E-cadherin and vimentin were evaluated by Western blot (WB). MTT, Transwell and wound-healing experiments were adopted to detect cell proliferation, invasion and migration. …”
Publicado 2020
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129978“…Among seizures with known sources (N = 355), the most frequent geographic region given as the source of substances was Eastern Europe (47.6%), followed by East Asia (24.8%), West Asia (19.4%), and Western Europe (5.9%). Bulgaria was the country with the highest frequency of seizures (N = 71) followed by Jordan (N = 45), Thailand (N = 37) and Moldova (N = 36). …”
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129979por Zhou, Meng, Wang, Xiaoyu, Xia, Jie, Cheng, Yating, Xiao, Lichun, Bei, Yu, Tang, Jianzhong, Huang, Yadong, Xiang, Qi, Huang, Shiliang“…Androgen receptor (AR) and prostate-specific antigen (PSA) expression was measured by Western blotting. CS-4D5/6e-based inhibition of AKR1C3 was evaluated in tumor-xenografted mice. …”
Publicado 2020
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129980por Ormazabal, Paulina, Sanhueza, Sofía, Herrera, Karin, Cifuentes, Mariana, Varì, Rosaria, Scazzocchio, Beatrice, Masella, Roberta, Paredes, Adrián, Morales, Glauco“…FABP4 content was assessed by Western blot. The levels of TNF-α and IL-6 in the culture media were analysed by ELISA. …”
Publicado 2020
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