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  1. 130321
    “…Moreover, postsynaptic densities were isolated from embryonic Vti1a(−/−) Vti1b(−/−) and Vti1a(+/−) Vti1b(+/−) control forebrains and analyzed by western blotting. RESULTS: Golgi outposts were present in Vti1a(−/−) Vti1b(+/−) and Vti1a(+/−) Vti1b(−/−) dendrites of hippocampal neurons but not detected in the absence of vti1a and vti1b. …”
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    Primary neurons lacking the SNAREs vti1a and vti1b show altered neuronal development
  2. 130322
    “…HR activity was evaluated using a plasmid-based assay, DNA breaks assessed by evaluating expression of γ-H2AX (a marker of DNA breaks) and p-RPA32 (a marker of DNA end resection) using Western blotting. Genomic instability was monitored by investigating micronuclei (a marker of genomic instability). …”
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    RAD51 Is Implicated in DNA Damage, Chemoresistance and Immune Dysregulation in Solid Tumors
  3. 130323
    “…As a result, iMatrix-511 increased the expression of P190 RhoGAP (GTPase-activating proteins) mRNA, such as ARHGAP1 mRNA (mean [SD]: iMatrix-511, 1.00 [0.57]; collagen+MFGE8, 0.032 [0.03]; **P < 0.01; n = 6), ARHGAP4 mRNA (mean [SD]: iMatrix-511, 1.00 [0.56]; collagen+MFGE8, 0.039 [0.049]; **P < 0.01; n = 6), and ARHGAP5 mRNA (mean [SD]: iMatrix-511, 1.00 [0.39]; collagen+MFGE8, 0.063 [0.043]; **P < 0.01; n = 6). Western blotting showed that phospho-Rac1 remained in the cytoplasm and phospho-Fyn showed nuclear transition in iPSCs cultured on iMatrix-511. …”
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    Laminin-511 Activates the Human Induced Pluripotent Stem Cell Survival via α6β1 Integrin-Fyn-RhoA-ROCK Signaling
  4. 130324
    “…Then Histology, enzyme-linked immunosorbent assays, and western blots were performed to determine these compounds’ anti-hypoxic effects and mechanisms. …”
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    Preliminary verification of the anti-hypoxia mechanism of Gentiana straminea maxim based on UPLC-triple TOF MS/MS and network pharmacology
  5. 130325
    “…These findings expand our knowledge about the effects of SIs on loneliness and social isolation in non-Western populations. Interventions targeting HI only and DSI might be particularly effective for mitigating loneliness of older adults in urban and rural areas, respectively. …”
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    Dual Sensory Impairment as a Predictor of Loneliness and Isolation in Older Adults: National Cohort Study
  6. 130326
    “…Gain- and loss-of-function experiments were performed to explore the role of miR-181a in BC progression with the involvement of the AKT signaling pathway. Western blotting, enzyme-linked immunosorbent assay, RT-qPCR, flow cytometry staining, migration assay, immunohistochemical staining, and bioinformatics analysis were performed to reveal the underlying mechanisms. …”
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    Monocytes educated by cancer-associated fibroblasts secrete exosomal miR-181a to activate AKT signaling in breast cancer cells
  7. 130327
    “…The primary efficacy endpoint for this study was change from baseline in WOMAC (Western Ontario and McMaster Universities Osteoarthritis Index) scores at month 11 whereas the secondary efficacy endpoints were change from baseline of VAS pain scale at months 1, 2, 5, 8, and 11 as well as change from baseline of WOMAC, KOOS (Knee and Osteoarthritis Outcome System), and IKDC (International Knee Documentation Committee) scale at month 5, 8, and 11. …”
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    An Open-Labeled Randomized Prospective Multi-center Study to Evaluate the Efficacy and Safety of Intra-articular Injection of OSSINEXT™, an Autologous Growth Factor Concentrate (AGFC) Compared to Hyaluronic Acid (HA) in Knee Osteoarthritis
  8. 130328
    “…BACKGROUND: Previous studies, predominantly in Western individuals, have reported weight gain or weight loss are related to the increased depressive symptoms at all ages, but no study of depressive symptoms has examined its relation to actual (not just self-reported) weight changes in the middle-aged and older adults. …”
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    Weight changes and the incidence of depressive symptom in the middle-aged and older adults: findings from the Chinese nationwide cohort study
  9. 130329
    “…We used cell viability, invasion assays, immunofluorescence, Western blotting, qRT-PCRs, flow cytometry and cell sorting, high-throughput bulk RNA sequencing and in vivo tumorigenesis to investigate YAP dependency and response of BRAF-mutant cells to vemurafenib. …”
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    Yap governs a lineage-specific neuregulin1 pathway-driven adaptive resistance to RAF kinase inhibitors
  10. 130330
    “…Co-expression of MIR29B and MYB in both cell types further demonstrated the inhibitory effect of MIR29B on MYB expression, resulting in HBG reactivation by real-time PCR, Western blot, and flow cytometry analysis. Finally, we confirmed the ability of MIR29B to reduce sickling and induce HbF by decreasing expression of MYB and DNMT3 gene expression in the humanized Townes sickle cell mouse model. …”
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    MicroRNA29B induces fetal hemoglobin via inhibition of the HBG repressor protein MYB in vitro and in humanized sickle cell mice
  11. 130331
    “…However, the role of COL6A2 in glioma and the relationship between COL6A2 and tumor infiltrating immune cells remain unclear. METHODS: Western blot, real-time PCR, a tissue microarray and immunohistochemistry were applied to detect COL6A2 mRNA and protein amounts in glioma, and all experiments were repeated three times. …”
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    The pro-invasive factor COL6A2 serves as a novel prognostic marker of glioma
  12. 130332
    “…Spleen from Fas(lpr/lpr) and OPN-/-Fas(lpr/lpr) mice, as well as purified B cells, were analysed by histopathology, flow cytometry, Western Blot, immunohistochemistry, immunofluorescence and gene expression profile to define lymphoma characteristics and investigate the molecular mechanisms behind the observed phenotype. …”
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    Intracellular osteopontin protects from autoimmunity-driven lymphoma development inhibiting TLR9-MYD88-STAT3 signaling
  13. 130333
    “…In the adjusted model, the odds of responding were lower at research sites in the Western (aOR 0.35 95% CI 0.21-0.57) and Southern US (aOR 0.29 95% CI 0.19-0.44, ref=Midwest), in women of Hispanic ethnicity (aOR 0.47 95% CI 0.33-0.66, ref=Black race), and in those who reported substance use (aOR 0.62 95% CI 0.40-0.95). …”
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    1903. Factors associated with response to a phone-administered alcohol and substance use survey during the COVID-19 pandemic among women in the MACS/WIHS Combined Cohort Study: Who are we missing?
  14. 130334
    “…CONCLUSIONS: Rates of hospitalization, ICU-admission, in-hospital mortality and overall case fatality were nearly comparable to the rates in western countries. Early interventions are necessary for high-risk Covid-19 patients, especially elderly patients and those with cardiac diseases.…”
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    Clinical characteristics and outcome of Covid-19 illness and predictors of in-hospital mortality in Saudi Arabia
  15. 130335
    “…The expression of ADAR1, EV-D68 VP1, and HPIV3 hemagglutinin–neuraminidase (HN) proteins was identified using western blotting. The median tissue culture infectious dose (TCID(50)) was applied to detect viral titers. …”
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    ADAR1p110 promotes Enterovirus D68 replication through its deaminase domain and inhibition of PKR pathway
  16. 130336
    “…In which we validated by qRT-PCR and western blotting that the cholesterol program was markedly activated. …”
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    Sodium butyrate alleviates deoxynivalenol-induced hepatic cholesterol metabolic dysfunction via RORγ-mediated histone acetylation modification in weaning piglets
  17. 130337
    “…Anchorage independent assay, Western blot, RNA interference, confocal analysis and neutralizing antibody treatment were performed to analyze the effects of POSTN on tumor promotion and to explore the underlying mechanism. …”
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    Periostin promotes ovarian cancer metastasis by enhancing M2 macrophages and cancer-associated fibroblasts via integrin-mediated NF-κB and TGF-β2 signaling
  18. 130338
    “…The study population was representative of a Western European HIV population, including 288 (15.2%) cis-women, 463 (24.4%) non-whites, and 1360 (71.8%) MSM (Men who have Sex with Men). …”
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    The 2000HIV study: Design, multi-omics methods and participant characteristics
  19. 130339
    “…The roles of p85S6K in synaptic targeting of GluA1 and learning and memory were evaluated by specific knockdown or overexpression of p85S6K followed by a broad range of methodologies including immunofluorescence, Western blot, in situ proximity ligation assay, morphological staining and behavioral examination. …”
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    p85S6K sustains synaptic GluA1 to ameliorate cognitive deficits in Alzheimer’s disease
  20. 130340
    “…Based on the difference in genetic makeup and demographic patterns in Africans compared to the Western world and with the rising burden of DM and other non‐communicable diseases in Ghana there is a need to define the impact DM has on persons with COVID‐19. …”
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    Clinical and laboratory profile and outcomes of hospitalized COVID‐19 patients with type 2 diabetes mellitus in Ghana – A single‐center study
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