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  1. 130421
    “…The expression of ANP, NPR-A, and NPR-C in kidney was evaluated with western blot analysis. Regarding renal redox state, the concentration changes in malondialdehyde (MDA), lipofuscin, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (Nox), and nitric oxide synthase (NOS) in kidney were detected by a spectrophotometric method. …”
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    High salt-induced weakness of anti-oxidative function of natriuretic peptide receptor-C and podocyte damage in the kidneys of Dahl rats
  2. 130422
    “…Patient reported outcomes included the 12-item Short Form Health Survery (SF-12) Physical Composite Scale (PCS) and Mental Health Composite Scale (MCS), the Western Ontario and McMaster University Osteoarthritis Index (WOMAC), International Knee Documentation Committee Questionnaire (IKDC), Lysholm Knee Questionnaire, Tegner Activity Scale and patient satisfaction. …”
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    Post-operative outcomes associated with posterolateral tibial plateau impaction fractures in the setting of primary ACL tear
  3. 130423
    por Ogunleye, Olayinka O., Basu, Debashis, Mueller, Debjani, Sneddon, Jacqueline, Seaton, R. Andrew, Yinka-Ogunleye, Adesola F., Wamboga, Joshua, Miljković, Nenad, Mwita, Julius C., Rwegerera, Godfrey Mutashambara, Massele, Amos, Patrick, Okwen, Niba, Loveline Lum, Nsaikila, Melaine, Rashed, Wafaa M., Hussein, Mohamed Ali, Hegazy, Rehab, Amu, Adefolarin A., Boahen-Boaten, Baffour Boaten, Matsebula, Zinhle, Gwebu, Prudence, Chirigo, Bongani, Mkhabela, Nongabisa, Dlamini, Tenelisiwe, Sithole, Siphiwe, Malaza, Sandile, Dlamini, Sikhumbuzo, Afriyie, Daniel, Asare, George Awuku, Amponsah, Seth Kwabena, Sefah, Israel, Oluka, Margaret, Guantai, Anastasia N., Opanga, Sylvia A., Sarele, Tebello Violet, Mafisa, Refeletse Keabetsoe, Chikowe, Ibrahim, Khuluza, Felix, Kibuule, Dan, Kalemeera, Francis, Mubita, Mwangana, Fadare, Joseph, Sibomana, Laurien, Ramokgopa, Gwendoline Malegwale, Whyte, Carmen, Maimela, Tshegofatso, Hugo, Johannes, Meyer, Johanna C., Schellack, Natalie, Rampamba, Enos M., Visser, Adel, Alfadl, Abubakr, Malik, Elfatih M., Malande, Oliver Ombeva, Kalungia, Aubrey C., Mwila, Chiluba, Zaranyika, Trust, Chaibva, Blessmore Vimbai, Olaru, Ioana D., Masuka, Nyasha, Wale, Janney, Hwenda, Lenias, Kamoga, Regina, Hill, Ruaraidh, Barbui, Corrado, Bochenek, Tomasz, Kurdi, Amanj, Campbell, Stephen, Martin, Antony P., Phuong, Thuy Nguyen Thi, Thanh, Binh Nguyen, Godman, Brian
    Publicado 2020
    “…ONGOING ACTIVITIES: Prevalence and mortality rates are currently lower in Africa than among several Western countries and the USA. This could be due to a number of factors including early instigation of lockdown and border closures, the younger age of the population, lack of robust reporting systems and as yet unidentified genetic and other factors. …”
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    Response to the Novel Corona Virus (COVID-19) Pandemic Across Africa: Successes, Challenges, and Implications for the Future
  4. 130424
    “…Methods: Primary Pten-deficient (and corresponding wild-type) mouse embryonic fibroblasts (MEFs) and astrocytes and PTEN-null human tumor cell lines and primary cells were assessed for RAD51 expression (via the Western blot analysis) and DNA damage repair analyses (via alkali comet and γH2AX foci assays). …”
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    RAD51-Mediated DNA Homologous Recombination Is Independent of PTEN Mutational Status
  5. 130425
    “…MMP-9 secretion was analyzed by gel electrophoresis/western blot and quantitated by ImageJ software. TNFα secretion was analyzed by enzyme-linked immuno sorbent assay. …”
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    Inhibition of matrix metalloproteinase-9 secretion by dimethyl sulfoxide and cyclic adenosine monophosphate in human monocytes
  6. 130426
    “…FUNDING: The Toronto COVID-19 Action Initiative, University of Toronto, and the Ontario First COVID-19 Rapid Research Fund, Toronto General & Western Hospital Foundation.…”
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    Peginterferon lambda for the treatment of outpatients with COVID-19: a phase 2, placebo-controlled randomised trial
  7. 130427
    “…ADSCs were characterized by using flow cytometric analysis and adipogenic and osteogenic differentiation assays. sEV was identified by electron microscopy, nanoparticle tracking, and western blotting. ADSCs were treated with sEV-LT and sEV-AT, respectively. …”
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    Therapeutic potential of small extracellular vesicles derived from lipoma tissue in adipose tissue regeneration—an in vitro and in vivo study
  8. 130428
    “…. = 2.287, 95%CI: 0.729, 3.845; OR = 1.217, 95%CI: 1.008, 1.469) rural residents living in western area (Coef. = 2.098, 95%CI: 0.464, 3.732; OR = 1.276, 95%CI: 1.044, 1.560) with higher social status (Coef. = 1.158, 95%CI: 0.756, 1.561; OR = 1.145, 95%CI: 1.090, 1.204), evaluation on adequacy (Coef. = 7.018, 95%CI: 5.045, 8.992; OR = 2.163, 95%CI: 1.719, 2.721), distribution (Coef. = 4.464, 95%CI: 2.471, 6.456; OR = 1.658, 95%CI: 1.312, 2.096) and accessibility (Coef. = 2.995, 95%CI: 0.963, 5.026; OR = 1.525, 95%CI: 1.217, 1.911) of healthcare resources had better patient experience. …”
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    Rural and urban differences in patient experience in China: a coarsened exact matching study from the perspective of residents
  9. 130429
    “…Expression alterations were examined via microarray, RNA sequencing and reverse transcription quantitative real-time PCR (qRT-PCR), proteins were analysed by western blotting and immunostaining, and insulin secretion was quantified in static incubations. …”
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    The hepatokine fetuin-A disrupts functional maturation of pancreatic beta cells
  10. 130430
    “…And the protein expression of UCP1, CD36, fatty acid transporter 4 (FATP4), peroxisome proliferator-activated receptor alpha (PPARα) and prohibitin of scWAT and BAT were analyzed using western blotting. In addition, ultra-high-performance liquid chromatography with electrospray ionization tandem mass spectrometry was utilized to detect the small lipid metabolites of scWAT and BAT. …”
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    Emodin Improves Glucose and Lipid Metabolism Disorders in Obese Mice via Activating Brown Adipose Tissue and Inducing Browning of White Adipose Tissue
  11. 130431
    “…METHODS: CHD1L mRNA-targeted siRNA was designed and induced a decreased level of CHD1L function in SK-OV-3 and OVCAR-3 cells observed via transwell and wound healing assays and assessment of epithelial–mesenchymal transition (EMT)-related protein expression by immunofluorescence (IF) and western blotting (WB). After decreasing the level of CHD1L, RNA-seq was conducted, and the circRNA expression profiles were obtained. cirRNAs were then selected and validated by PCR together with Sanger sequencing, fluorescent in situ hybridization (FISH), and reverse transcriptase-quantitative PCR (RT-qPCR). …”
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    circ-PTK2 (hsa_circ_0008305) regulates the pathogenic processes of ovarian cancer via miR-639 and FOXC1 regulatory cascade
  12. 130432
    “…Immunohistochemistry (CD11b, GFAP, Aβ, NeuN, NeuroChrom) and Tau (S)-[(18)F]THK5117 autoradiography, immunohistochemistry and Western blot were also performed. Results: [(18)F]DPA-714 positron emission tomography (PET) showed an increase in neuroinflammation in TG vs wildtype animals from 12 m in the hippocampus (+11%), and at the advanced-stage AD in the hippocampus (+12%), the thalamus (+11%) and frontal cortex (+14%). …”
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    Prodromal neuroinflammatory, cholinergic and metabolite dysfunction detected by PET and MRS in the TgF344-AD transgenic rat model of AD: a collaborative multi-modal study
  13. 130433
    “…FUNDING: National Cancer Institute of the US National Institutes of Health, National Institute for Biomedical Imaging and Bioengineering, National Center for Research Resources, US Department of Veterans Affairs Biomedical Laboratory Research and Development Service, US Department of Defense, US National Institute of Diabetes and Digestive and Kidney Diseases, The Ohio Third Frontier Technology Validation Fund, Case Western Reserve University, Dana Foundation, and Clinical and Translational Science Collaborative.…”
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    An integrated nomogram combining deep learning, Prostate Imaging–Reporting and Data System (PI-RADS) scoring, and clinical variables for identification of clinically significant prostate cancer on biparametric MRI: a retrospective multicentre study
  14. 130434
    “…As the most populous country in the Western Pacific region, it is warranted to conduct a study with a large scale of patients in a real-life setting. …”
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    Effectiveness and safety of iguratimod treatment in patients with active rheumatoid arthritis in Chinese: A nationwide, prospective real-world study
  15. 130435
    “…Patients were also asked to indicate if they experienced pain or cramping on a VAS, and complete the American Shoulder and Elbow Score (ASES) and the Western Ontario Rotator Cuff score (WORC). Isometric elbow flexion and supination strength (ratio of affected and unaffected) were also measured. …”
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    Effect of Age, Gender, And BMI on the Incidence and Satisfaction of A Popeye Deformity Following Biceps Tenotomy or Tenodesis: Outcomes of A Multicenter Randomized Controlled Trial
  16. 130436
    “…The primary composite effectiveness endpoint was defined as achievement of the minimal clinically important difference (MCID) for the Western Ontario Rotator Cuff (WORC) score (275 points) and American Shoulder and Elbow Society (ASES) score (6.4 points) by week 6 and maintenance at month 12 without subsequent secondary surgical intervention (SSSI) or serious adverse device effects (SADEs). …”
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    Subacromial Balloon Spacer versus Partial Repair for Massive Rotator Cuff Tears: A Prospective, Randomized, Multi-center Trial
  17. 130437
    “…American Shoulder and Elbow Surgeon (ASES), Disability of the Arm Shoulder and Hand (DASH), and Western Ontario Rotator Cuff Index (WORC) surveys were completed before (PRE) and 1-year (1YR-POST) after SCR. …”
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    Reduced Movement Variability and Kinematics Changes During a Hand to Head Movement are Associated with Improved Outcome Following Superior Capsular Reconstruction (232)
  18. 130438
    “…Relevant expression levels were determined by the qRT-PCR and Western blotting. CCK-8 assay, flow cytometry, and transwell migration assays were performed to assess cell proliferation, apoptosis, and migration. …”
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    Mir24-2-5p suppresses the osteogenic differentiation with Gnai3 inhibition presenting a direct target via inactivating JNK-p38 MAPK signaling axis
  19. 130439
    “…METHODS: At first, dose–response (0, 10, 20, and 30 μg/mL) and time-response (0, 3, 6, 12, and 24 h) studies were performed in immortalized normal human bronchial epithelial cells BEAS-2B to observe the effects of Nano-Ni on DNA damage response (DDR)-associated proteins and the HIF-1α/miR-210/Rad52 pathway by real-time PCR or Western blot. Then, a Hsp90 inhibitor (1 µM of 17-AAG, an indirect HIF-1α inhibitor), HIF-1α knock-out (KO) cells, and a miR-210 inhibitor (20 nM) were used to determine whether Nano-Ni-induced Rad52 down-regulation was through HIF-1α nuclear accumulation and miR-210 up-regulation. …”
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    Nickel nanoparticle-induced cell transformation: involvement of DNA damage and DNA repair defect through HIF-1α/miR-210/Rad52 pathway
  20. 130440
    “…Recombinant PoMSP1(19) (rPoMSP1(19))-glutathione S-transferase (GST) proteins were expressed in an Escherichia coli expression system and analyzed by western blot. Immune responses in BALB/c mice immunized with rPoMSP1(19)-GST were determined using enzyme-linked immunosorbent assay. …”
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    Genetic diversity and immunogenicity of the merozoite surface protein 1 C-terminal 19-kDa fragment of Plasmodium ovale imported from Africa into China
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